Journal of Cell Science, Vol 104, Issue 2 383-389, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Gamma-tubulin reorganization during mouse fertilization and early development

MJ Palacios, HC Joshi, C Simerly and G Schatten
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322.

gamma-Tubulin, a component of spindle pole bodies in fungal cells and pericentriolar material in vertebrate cells, is thought to play a role in the nucleation of microtubule growth and to define their polarity. In contrast to the adult somatic cells, microtubules are nucleated in the absence of centrioles in mammalian oocytes and early embryos. By studying acentriolar mouse oocytes and their early development following fertilization, we show that gamma-tubulin antibody crossreacts with a 50,000 M(r) protein in unfertilized mouse oocytes and demonstrate that gamma-tubulin distribution is rearranged dramatically during fertilization. In unfertilized mouse oocytes, gamma-tubulin is concentrated in the broad spindle poles of meiotic spindle (MII) and as the distinct foci which form the centers of the cytoplasmic microtubule asters (cytasters). The integrity of these gamma-tubulin foci and their cytoplasmic location is maintained during the drug- or cold-induced depolymerization of microtubules. gamma-Tubulin is also found in the basal body of the mouse sperm. During fertilization, the gamma-tubulin is found at the cytastral centers as well as in the incorporated sperm basal body complex, and the gamma-tubulin foci coalesce at the perinuclear microtubule organizing regions of the two pronuclei at the first mitotic prophase. During mitosis, gamma-tubulin is found associated with broad bands that form the poles of the first mitotic spindle. By the late preimplantation stage, when newly generated centrioles have been reported to arise, gamma-tubulin remains localized at the centrosome of mitotic cells.(ABSTRACT TRUNCATED AT 250 WORDS)
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. Moutinho-Pereira, A. Debec, and H. Maiato Microtubule Cytoskeleton Remodeling by Acentriolar Microtubule-organizing Centers at the Entry and Exit from Mitosis in Drosophila Somatic Cells Mol. Biol. Cell, June 1, 2009; 20(11): 2796 - 2808. [Abstract] [Full Text] [PDF]

				J.-W. Yang, Z.-L. Lei, Y.-L. Miao, J.-C. Huang, L.-H. Shi, Y.-C. OuYang, Q.-Y. Sun, and D.-Y. Chen Spindle assembly in the absence of chromosomes in mouse oocytes Reproduction, December 1, 2007; 134(6): 731 - 738. [Abstract] [Full Text] [PDF]

				S. L. Barrett and D. F. Albertini Allocation of Gamma-Tubulin Between Oocyte Cortex and Meiotic Spindle Influences Asymmetric Cytokinesis in the Mouse Oocyte Biol Reprod, June 1, 2007; 76(6): 949 - 957. [Abstract] [Full Text] [PDF]

				G Manandhar, D Feng, Y-J Yi, L Lai, J Letko, J Laurincik, M Sutovsky, J L Salisbury, R S Prather, H Schatten, et al. Centrosomal protein centrin is not detectable during early pre-implantation development but reappears during late blastocyst stage in porcine embryos. Reproduction, September 1, 2006; 132(3): 423 - 434. [Abstract] [Full Text] [PDF]

				N. Van Thuan, S. Wakayama, S. Kishigami, and T. Wakayama Donor Centrosome Regulation of Initial Spindle Formation in Mouse Somatic Cell Nuclear Transfer: Roles of Gamma-Tubulin and Nuclear Mitotic Apparatus Protein 1 Biol Reprod, May 1, 2006; 74(5): 777 - 787. [Abstract] [Full Text] [PDF]

				X. Li, Y Qin, S. Wilsher, and W R Allen Centrosome changes during meiosis in horse oocytes and first embryonic cell cycle organization following parthenogenesis, fertilization and nuclear transfer. Reproduction, April 1, 2006; 131(4): 661 - 667. [Abstract] [Full Text] [PDF]

				G. Manandhar, H. Schatten, and P. Sutovsky Centrosome Reduction During Gametogenesis and Its Significance Biol Reprod, January 1, 2005; 72(1): 2 - 13. [Abstract] [Full Text] [PDF]

				H. Miki, K. Inoue, N. Ogonuki, K. Mochida, H. Nagashima, T. Baba, and A. Ogura Cytoplasmic Asters Are Required for Progression Past the First Cell Cycle in Cloned Mouse Embryos Biol Reprod, December 1, 2004; 71(6): 2022 - 2028. [Abstract] [Full Text] [PDF]

				J. Lee, T. Miyano, and R. M. Moor Spindle Formation and Dynamics of {gamma}-Tubulin and Nuclear Mitotic Apparatus Protein Distribution During Meiosis in Pig and Mouse Oocytes Biol Reprod, May 1, 2000; 62(5): 1184 - 1192. [Abstract] [Full Text]

				J. L. Paluh, E. Nogales, B. R. Oakley, K. McDonald, A. L. Pidoux, and W. Z. Cande A Mutation in gamma -Tubulin Alters Microtubule Dynamics and Organization and Is Synthetically Lethal with the Kinesin-like Protein Pkl1p Mol. Biol. Cell, April 1, 2000; 11(4): 1225 - 1239. [Abstract] [Full Text]

				Y. Terada, C. R. Simerly, L. Hewitson, and G. Schatten Sperm Aster Formation and Pronuclear Decondensation During Rabbit Fertilization and Development of a Functional Assay for Human Sperm Biol Reprod, March 1, 2000; 62(3): 557 - 563. [Abstract] [Full Text]

				C. Simerly, S. S. Zoran, C. Payne, T. Dominko, P. Sutovsky, C. S. Navara, J. L. Salisbury, and G. Schatten Biparental Inheritance of gamma -Tubulin during Human Fertilization: Molecular Reconstitution of Functional Zygotic Centrosomes in Inseminated Human Oocytes and in Cell-free Extracts Nucleated by Human Sperm Mol. Biol. Cell, September 1, 1999; 10(9): 2955 - 2969. [Abstract] [Full Text]

				M. R. Dow and P. E. Mains Genetic and Molecular Characterization of the Caenorhabditis elegans Gene, mel-26, a Postmeiotic Negative Regulator of MEI-1, a Meiotic-Specific Spindle Component Genetics, September 1, 1998; 150(1): 119 - 128. [Abstract] [Full Text]

				Z.-h. Yang, G. I. Gallicano, Q.-C. Yu, and E. Fuchs An Unexpected Localization of Basonuclin in the Centrosome, Mitochondria, and Acrosome of Developing Spermatids J. Cell Biol., May 5, 1997; 137(3): 657 - 669. [Abstract] [Full Text] [PDF]

				V Scott, T Sherwin, and K Gull gamma-tubulin in trypanosomes: molecular characterisation and localisation to multiple and diverse microtubule organising centres J. Cell Sci., January 1, 1997; 110(2): 157 - 168. [Abstract] [PDF]

				I Lajoie-Mazenc, C Detraves, V Rotaru, M Gares, Y Tollon, C Jean, M Julian, M Wright, and B Raynaud-Messina A single gamma-tubulin gene and mRNA, but two gamma-tubulin polypeptides differing by their binding to the spindle pole organizing centres J. Cell Sci., January 10, 1996; 109(10): 2483 - 2492. [Abstract] [PDF]

				H Masuda and T Shibata Role of gamma-tubulin in mitosis-specific microtubule nucleation from the Schizosaccharomyces pombe spindle pole body J. Cell Sci., January 1, 1996; 109(1): 165 - 177. [Abstract] [PDF]

				K. Wolf and H. Joshi Microtubule organization and the distribution of gamma-tubulin in spermatogenesis of a beetle, Tenebrio molitor (Tenebrionidae, Coleoptera, Insecta) J. Cell Sci., January 12, 1995; 108(12): 3855 - 3865. [Abstract] [PDF]

				A Debec, C Detraves, C Montmory, G Geraud, and M Wright Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells J. Cell Sci., January 7, 1995; 108(7): 2645 - 2653. [Abstract] [PDF]

				R. Burns Analysis of the gamma-tubulin sequences: implications for the functional properties of gamma-tubulin J. Cell Sci., January 6, 1995; 108(6): 2123 - 2130. [PDF]

				A Vassilev, M Kimble, C. Silflow, M LaVoie, and R Kuriyama Identification of intrinsic dimer and overexpressed monomeric forms of gamma-tubulin in Sf9 cells infected with baculovirus containing the Chlamydomonas gamma-tubulin sequence J. Cell Sci., January 3, 1995; 108(3): 1083 - 1092. [Abstract] [PDF]

				I Lajoie-Mazenc, Y Tollon, C Detraves, M Julian, A Moisand, C Gueth-Hallonet, A Debec, I Salles-Passador, A Puget, H Mazarguil, et al. Recruitment of antigenic gamma-tubulin during mitosis in animal cells: presence of gamma-tubulin in the mitotic spindle J. Cell Sci., January 10, 1994; 107(10): 2825 - 2837. [Abstract] [PDF]