|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 104, Issue 2 415-425, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
J Baker and D Garrod
Cancer Research Campaign, Medical Oncology Unit, University of Southampton, Southampton General Hospital, UK.
It has long been known that cells show reduced cell-substratum adhesion during mitosis in tissue culture, but it is not generally known whether cell-cell adhesion is also reduced. Epithelial cells, both in culture and in tissues, are linked together by several different types of intercellular junctions. Are these junctions disassembled when epithelial cells divide? Cultured epithelial cells were fluorescently stained for desmosomes, tight junctions and zonulae adherentes, and large numbers of dividing cells examined by light microscopy. The results suggested that all three types of intercellular junctions were retained throughout cell division and no evidence for internalization of junctions was obtained. The persistence of intercellular junctions by cultured cells during division was confirmed by electron microscopy. In order to determine whether intercellular junctions were similarly retained by dividing cells in tissues, human colonic mucosal crypt cells and basal keratinocytes were studied by electron microscopy. Both cell types retained intercellular junctions during division. Dividing basal keratinocytes also possessed hemidesmosomal contact with the basement membrane. It is suggested that retention of cellular junctions during division is important for maintenance of tissue integrity and organization.
This article has been cited by other articles:
|
|
 |
|
  S. J. Baines, E. F. McInnes, and I. McConnell E-cadherin expression in canine cutaneous histiocytoma Vet Rec., July 12, 2008; 163(2): 60 - 60. [Full Text] [PDF]
|
|
|
|
 |
|
 X. Guo, J. N. Rao, L. Liu, T. Zou, K. M. Keledjian, D. Boneva, B. S. Marasa, and J.-Y. Wang Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1159 - G1169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Matsuda, A. Kubo, M. Furuse, and S. Tsukita A peculiar internalization of claudins, tight junction-specific adhesion molecules, during the intercellular movement of epithelial cells J. Cell Sci., March 1, 2004; 117(7): 1247 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. W. Geuijen and A. Sonnenberg Dynamics of the alpha 6beta 4 Integrin in Keratinocytes Mol. Biol. Cell, November 1, 2002; 13(11): 3845 - 3858. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Windoffer, M. Borchert-Stuhltrager, and R. E. Leube Desmosomes: interconnected calcium-dependent structures of remarkable stability with significant integral membrane protein turnover J. Cell Sci., April 15, 2002; 115(8): 1717 - 1732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kojima, Y. Kokai, H. Chiba, M. Osanai, K. Kuwahara, M. Mori, Y. Mochizuki, and N. Sawada Occludin and Claudin-1 Concentrate in the Midbody of Immortalized Mouse Hepatocytes During Cell Division J. Histochem. Cytochem., March 1, 2001; 49(3): 333 - 340. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Bauer, H. Lickert, R. Kemler, and J. Stappert Modification of the E-cadherin-Catenin Complex in Mitotic Madin-Darby Canine Kidney Epithelial Cells J. Biol. Chem., October 23, 1998; 273(43): 28314 - 28321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Wong and B. M. Gumbiner A Synthetic Peptide Corresponding to the Extracellular Domain of Occludin Perturbs the Tight Junction Permeability Barrier J. Cell Biol., January 27, 1997; 136(2): 399 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. DePasquale, W. Samsonoff, and J. Gierthy 17-beta-Estradiol induced alterations of cell-matrix and intercellular adhesions in a human mammary carcinoma cell line J. Cell Sci., January 5, 1994; 107(5): 1241 - 1254. [Abstract] [PDF]
|
|
