|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 104, Issue 4 1145-1153, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
DE Coan, AR Wechezak, RF Viggers and LR Sauvage
Hope Heart Research Institute, Seattle, Washington 98122.
Despite substantial evidence to suggest that directed cell migration is dependent upon positioning of the Golgi apparatus (GA) and the microtubule organizing center (MTOC), some controversy exists about whether such a relationship is relevant to endothelial cells under flow. The present study was undertaken to provide an indepth investigation of the relationship between shear stress, GA/MTOC localization, cell migration and nuclear position. Bovine carotid endothelial cells were exposed to 22 or 88 dynes/cm2 for 0.5, 2, 8 or 24 h, and localization of their GA/MTOCs was determined relative to the direction of flow. In no-flow control specimens, (0, 0.5, 2, 8 and 24 h) there was no change in the equally distributed GA/MTOCs. In contrast, during the first 8 h at 88 dynes/cm2 and by 2 h at 22 dynes/cm2 there was a significant increase in the number of cells with GA/MTOCs localized upstream to flow direction. The effect was temporary, however, and by 24 h there was no significant difference between the no-flow, 22 and 88 dynes/cm2 specimens. Analysis of GA/MTOC localization with respect to the direction of cell migration determined that 72.5% of no-flow cells possessed GA/MTOCs localized to the sides of nuclei nearest the direction of migration. In contrast, 64% of the specimens shear stressed over the same time period had GA/MTOCs localized to the sides of nuclei opposite the direction of migration. These results suggest that positioning of the GA/MTOC in endothelial cells is not dependent completely upon the direction of migration.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  F. Pouthas, P. Girard, V. Lecaudey, T. B. N. Ly, D. Gilmour, C. Boulin, R. Pepperkok, and E. G. Reynaud In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum J. Cell Sci., July 15, 2008; 121(14): 2406 - 2414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Uttayarat, M. Chen, M. Li, F. D. Allen, R. J. Composto, and P. I. Lelkes Microtopography and flow modulate the direction of endothelial cell migration Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H1027 - H1035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Hotchkiss, A. W. Ashton, R. Mahmood, R. G. Russell, J. A. Sparano, and E. L. Schwartz Inhibition of Endothelial Cell Function in Vitro and Angiogenesis in Vivo by Docetaxel (Taxotere): Association with Impaired Repositioning of the Microtubule Organizing Center Mol. Cancer Ther., November 1, 2002; 1(13): 1191 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Hu, S. Li, J. Y.-J. Shyy, and S. Chien Sustained JNK activation induces endothelial apoptosis: studies with colchicine and shear stress Am J Physiol Heart Circ Physiol, October 1, 1999; 277(4): H1593 - H1599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. Irigoyen, D. Besser, and Y. Nagamine Cytoskeleton Reorganization Induces the Urokinase-type Plasminogen Activator Gene via the Ras/Extracellular Signal-regulated Kinase (ERK) Signaling Pathway J. Biol. Chem., January 17, 1997; 272(3): 1904 - 1909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Malek and S Izumo Mechanism of endothelial cell shape change and cytoskeletal remodeling in response to fluid shear stress J. Cell Sci., January 4, 1996; 109(4): 713 - 726. [Abstract] [PDF]
|
|
|
|
 |
|
 D. S. Ettenson and A. I. Gotlieb Basic Fibroblast Growth Factor Is a Signal for the Initiation of Centrosome Redistribution to the Front of Migrating Endothelial Cells at the Edge of an In Vitro Wound Arterioscler. Thromb. Vasc. Biol., April 1, 1995; 15(4): 515 - 521. [Abstract] [Full Text]
|
|
