|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 105, Issue 1 167-177, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
TL Lee, YC Lin, K Mochitate and F Grinnell
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical School, Dallas 75235.
To learn about the effects of tension on fibroblast function, we have been studying initial cellular responses to stress-relaxation. Human foreskin fibroblasts were cultured in anchored collagen matrices for 2 days, during which time mechanical stress developed. Subsequently, the matrices were dislodged; thereby allowing stress to dissipate. Within 5 min after initiating stress-relaxation, fibroblasts retracted their pseudopodia. At this time, we observed the disappearance of cellular stress fibers and the formation of actin clusters along the cell margins. The actin was found to be located inside 200 nm diameter vesicles that were budding from the cell surface. Vesicles isolated from the matrix after stress-relaxation contained prominent 24 kDa, 36 kDa (doublet), 45 kDa, and 135 kDa polypeptides. The 45 kDa polypeptide was the major component in the Triton-insoluble vesicle fraction and appeared to be actin. The 36 kDa (doublet) polypeptide, which was found predominantly in the Triton-soluble vesicle fraction, was identified as annexin II. Vesicles also contained annexin VI and beta 1 integrin receptors but not tubulin, vimentin, vinculin or annexin I. The results suggest that stress-relaxation of fibroblasts induces a novel ectocytotic process involving transient budding of intact, plasma membrane vesicles from the cell cortex. On the basis of their morphological and biochemical features, these vesicles may be analogous to the 'matrix vesicles' released by chondrocytes and could play a role in extracellular matrix remodeling after wound contraction.
This article has been cited by other articles:
|
|
 |
|
  R. L. Mellgren and X. Huang Fetuin A Stabilizes m-Calpain and Facilitates Plasma Membrane Repair J. Biol. Chem., December 7, 2007; 282(49): 35868 - 35877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yuge, K. Nasu, H. Matsumoto, M. Nishida, and H. Narahara Collagen gel contractility is enhanced in human endometriotic stromal cells: a possible mechanism underlying the pathogenesis of endometriosis-associated fibrosis Hum. Reprod., April 1, 2007; 22(4): 938 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Mellgren, W. Zhang, K. Miyake, and P. L. McNeil Calpain Is Required for the Rapid, Calcium-dependent Repair of Wounded Plasma Membrane J. Biol. Chem., January 26, 2007; 282(4): 2567 - 2575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Gasser and J. A. Schifferli Activated polymorphonuclear neutrophils disseminate anti-inflammatory microparticles by ectocytosis Blood, October 15, 2004; 104(8): 2543 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gerke and S. E. Moss Annexins: From Structure to Function Physiol Rev, April 1, 2002; 82(2): 331 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Falcone, W. Borth, K. M. F. Khan, and K. A. Hajjar Plasminogen-mediated matrix invasion and degradation by macrophages is dependent on surface expression of annexin II Blood, February 1, 2001; 97(3): 777 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mai, R. L. Finley Jr., D. M. Waisman, and B. F. Sloane Human Procathepsin B Interacts with the Annexin II Tetramer on the Surface of Tumor Cells J. Biol. Chem., April 21, 2000; 275(17): 12806 - 12812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Grinnell, C.-H. Ho, Y.-C. Lin, and G. Skuta Differences in the Regulation of Fibroblast Contraction of Floating Versus Stressed Collagen Matrices J. Biol. Chem., January 8, 1999; 274(2): 918 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Garoff, R. Hewson, and D.-J. E. Opstelten Virus Maturation by Budding Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1171 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S.F. Lee,, T. T. Wheeler,, and A. J. Peterson Large-Format, Two-Dimensional Polyacrylamide Gel Electrophoresis of Ovine Periimplantation Uterine Luminal Fluid Proteins: Identification of Aldose Reductase, Cytoplasmic Actin, and Transferrin as Conceptus-Synthesized Proteins Biol Reprod, October 1, 1998; 59(4): 743 - 752. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Rosenfeldt, D. J. Lee, and F. Grinnell Increased c-fos mRNA Expression By Human Fibroblasts Contracting Stressed Collagen Matrices Mol. Cell. Biol., May 1, 1998; 18(5): 2659 - 2667. [Abstract] [Full Text]
|
|
|
|
 |
|
 B Mehul and R. Hughes Plasma membrane targetting, vesicular budding and release of galectin 3 from the cytoplasm of mammalian cells during secretion J. Cell Sci., January 5, 1997; 110(10): 1169 - 1178. [Abstract] [PDF]
|
|
