|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 105, Issue 1 203-212, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
P Clark, S Britland and P Connolly
Department of Anatomy and Cell Biology, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London, UK.
Neurite growth cones detect and respond to guidance cues in their local environment that determine stereotyped pathways during development and regeneration. Micropatterns of laminin (which was found to adsorb preferentially to photolithographically defined hydrophobic areas of micropatterns) were here used to model adhesive pathways that might influence neurite extension. The responses of growth cones were determined by the degree of guidance of neurite extension and also by examining growth cone morphology. These parameters were found to be strongly dependent on the geometry of the patterned laminin, and on neuron type. Decreasing the spacing of multiple parallel tracks of laminin alternating with non-adhesive tracks, resulted in decreased guidance of chick embryo brain neurons. Single isolated 2 microns tracks strongly guided neurite extension whereas 2 microns tracks forming a 4 microns period multiple parallel pattern did not. Growth cones appear to be capable of bridging the narrow non-adhesive tracks, rendering them insensitive to the smaller period multiple parallel adhesive patterns. These observations suggest that growth cones would be unresponsive to the multiple adhesive cues such as would be presented by oriented extracellular matrix or certain axon fascicle structures, but could be guided by isolated adhesive tracks. Growth cone morphology became progressively simpler on progressively narrower single tracks. On narrow period multiple parallel tracks (which did not guide neurite extension) growth cones spanned a number of adhesive/non-adhesive tracks, and their morphology suggests that lamellipodial advance may be independent of the substratum by using filopodia as a scaffold. In addition to acting as guidance cues, laminin micropatterns also appeared to influence the production of primary neurites and their subsequent branching. On planar substrata, dorsal root ganglion neurons were multipolar, with highly branched neurite outgrowth whereas, on 25 microns tracks, neurite branching was reduced or absent, and neuron morphology was typically bipolar. These observations indicate the precision with which growth cone advance may be controlled by substrata and suggest a role for patterned adhesiveness in neuronal morphological differentiation, but also highlight some of the limitations of growth cone sensitivity to substratum cues.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Gehler, A. E. Shaw, P. D. Sarmiere, J. R. Bamburg, and P. C. Letourneau Brain-Derived Neurotrophic Factor Regulation of Retinal Growth Cone Filopodial Dynamics Is Mediated through Actin Depolymerizing Factor/Cofilin J. Neurosci., November 24, 2004; 24(47): 10741 - 10749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Wells and L. Lillien Attraction or Repulsion: A Matter of Individual Taste? Sci. Signal., October 5, 2004; 2004(253): pe47 - pe47. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. BHATIA, U. J. BALIS, M. L. YARMUSH, and M. TONER Effect of cell-cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells FASEB J, November 1, 1999; 13(14): 1883 - 1900. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. P. Farwell and S. A. Dubord-Tomasetti Thyroid Hormone Regulates the Expression of Laminin in the Developing Rat Cerebellum Endocrinology, September 1, 1999; 140(9): 4221 - 4227. [Abstract] [Full Text]
|
|
|
|
|
|
W. Halfter The Behavior of Optic Axons on Substrate Gradients of Retinal Basal Lamina Proteins and Merosin J. Neurosci., July 15, 1996; 16(14): 4389 - 4401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Webb, P Clark, J Skepper, A Compston, and A Wood Guidance of oligodendrocytes and their progenitors by substratum topography J. Cell Sci., January 8, 1995; 108(8): 2747 - 2760. [Abstract] [PDF]
|
|
|
|
|
|
T. Svitkina, Y. Rovensky, A. Bershadsky, and J. Vasiliev Transverse pattern of microfilament bundles induced in epitheliocytes by cylindrical substrata J. Cell Sci., January 2, 1995; 108(2): 735 - 745. [Abstract] [PDF]
|
|
