spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thiry, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thiry, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 105, Issue 1 33-39, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Ultrastructural distribution of DNA and RNA within the nucleolus of human Sertoli cells as seen by molecular immunocytochemistry

M Thiry
Laboratory of Cell and Tissue Biology, University of Liege, Belgium.

The precise distribution of DNA and RNA within the human Sertoli cell nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. In Sertoli cells, the nucleolar components show a typical spatial distribution. The fibrillar centres are not surrounded by a layer of dense fibrillar component, but come in contact only with strands of dense fibrillar component. These fibrillar parts of strands are the extensions of granular strands connected to a large granular mass. These strands delimit numerous nucleolar interstices in which chromatin fibres are clearly obvious. Using the in situ terminal deoxynucleotidyl transferase/immunogold procedure for detecting DNA, we find evident label exclusively over the chromatin fibres enclosed in the nucleolar interstices and over the fibrillar centres, and no significant label over the dense fibrillar component and granular component of the nucleolus. Furthermore, using the polyadenylate nucleotidyl transferase/immunogold procedure for detecting RNA, we show that label is deposited not only over the granular component and dense fibrillar component, as expected, but also quite obviously over the fibrillar centres. No label is seen over the interstices containing chromatin.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M.-S. Chang, H. Sasaki, M. S. Campbell, S.-K. Kraeft, R. Sutherland, C.-Y. Yang, Y. Liu, D. Auclair, L. Hao, H. Sonoda, et al.
HRad17 Colocalizes with NHP2L1 in the Nucleolus and Redistributes after UV Irradiation
J. Biol. Chem., December 17, 1999; 274(51): 36544 - 36549.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. A. Nickerson, G. Krockmalnic, K. M. Wan, and S. Penman
The nuclear matrix revealed by eluting chromatin from a cross-linked nucleus
PNAS, April 29, 1997; 94(9): 4446 - 4450.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A Majewska-Sawka and M. Rodriguez-Garcia
rRNA distribution during microspore development in anthers of Beta vulgaris L. quantitative in situ hybridization analysis
J. Cell Sci., January 4, 1996; 109(4): 859 - 866.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
S Besse and F Puvion-Dutilleul
Intranuclear retention of ribosomal RNAs in response to herpes simplex virus type 1 infection
J. Cell Sci., January 1, 1996; 109(1): 119 - 129.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
A Olmedilla, P. Testillano, O Vicente, M Delseny, and M. Risueno
Ultrastructural rRNA localization in plant cell nucleoli. RNA/RNA in situ hybridization, autoradiography and cytochemistry
J. Cell Sci., January 12, 1993; 106(4): 1333 - 1346.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993