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Journal of Cell Science, Vol 105, Issue 2 275-286, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
T Hashimoto, M Amagai, DA Parry, TW Dixon, S Tsukita, S Tsukita, K Miki, K Sakai, Y Inokuchi, J Kudoh and al. et
Department of Dermatology, Keio University School of Medicine, Shinjuku, Tokyo.
We have obtained a monoclonal antibody (33A-3D) that specifically recognize desmoyokin, a 680 kDa desmosomal plaque protein that is well characterized in bovine muzzle epidermis. A cDNA clone (DY6, 3693 bp) was isolated by immunoscreening a mouse keratinocyte expression library with 33A-3D, and it was confirmed that DY6 has a partial coding sequence for desmoyokin. DY6 consists of highly homologous repeats about 128 residues long. Furthermore, the 128-residue repeats exhibit a quasi seven-residue substructure, which we believe will adopt an antiparallel beta-sheet structure. Surprisingly, the amino acid sequence showed a significant homology with AHNAK, a newly identified human gene encoding a 700 kDa protein, which was suggested to be down-regulated in neuroblastoma. From its extensive homology, the similarity in both size and structure, and the identical patterns on Southern blot analysis of genomic DNAs, desmoyokin and AHNAK protein are thought to be identical. Although the desmoyokin/AHNAK protein is detected in a variety of cell types at both protein and mRNA levels, its distribution in keratinocytes (associated closely with cell membrane) is quite different from that in cells other than keratinocytes (distributed diffusely in the cytoplasm). These findings suggest that the desmoyokin/AHNAK protein is a ubiquitous molecule with a unique structure and appears to have different distributions (and probably different functions) among different cells.
This article has been cited by other articles:
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  Y. Huang, S. H. Laval, A. van Remoortere, J. Baudier, C. Benaud, L. V. B. Anderson, V. Straub, A. Deelder, R. R. Frants, J. T. den Dunnen, et al. AHNAK, a novel component of the dysferlin protein complex, redistributes to the cytoplasm with dysferlin during skeletal muscle regeneration FASEB J, March 1, 2007; 21(3): 732 - 742. [Abstract] [Full Text] [PDF]
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 H. Haase Ahnak, a new player in {beta}-adrenergic regulation of the cardiac L-type Ca2+ channel Cardiovasc Res, January 1, 2007; 73(1): 19 - 25. [Abstract] [Full Text] [PDF]
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 A. Komuro, Y. Masuda, K. Kobayashi, R. Babbitt, M. Gunel, R. A. Flavell, and V. T. Marchesi The AHNAKs are a class of giant propeller-like proteins that associate with calcium channel proteins of cardiomyocytes and other cells PNAS, March 23, 2004; 101(12): 4053 - 4058. [Abstract] [Full Text] [PDF]
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 C. Benaud, B. J. Gentil, N. Assard, M. Court, J. Garin, C. Delphin, and J. Baudier AHNAK interaction with the annexin 2/S100A10 complex regulates cell membrane cytoarchitecture J. Cell Biol., January 5, 2004; 164(1): 133 - 144. [Abstract] [Full Text] [PDF]
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 B. K. Straub, J. Boda, C. Kuhn, M. Schnoelzer, U. Korf, T. Kempf, H. Spring, M. Hatzfeld, and W. W. Franke A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells J. Cell Sci., December 15, 2003; 116(24): 4985 - 4995. [Abstract] [Full Text] [PDF]
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 B. J. Gentil, C. Delphin, C. Benaud, and J. Baudier Expression of the Giant Protein AHNAK (Desmoyokin) in Muscle and Lining Epithelial Cells J. Histochem. Cytochem., March 1, 2003; 51(3): 339 - 348. [Abstract] [Full Text] [PDF]
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A. HOHAUS, V. PERSON, J. BEHLKE, J. SCHAPER, I. MORANO, and H. HAASE The carboxyl-terminal region of ahnak provides a link between cardiac L-type Ca2+ channels and the actin-based cytoskeleton FASEB J, August 1, 2002; 16(10): 1205 - 1216. [Abstract] [Full Text] [PDF]
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J. Sussman, D. Stokoe, N. Ossina, and E. Shtivelman Protein kinase B phosphorylates AHNAK and regulates its subcellular localization J. Cell Biol., September 3, 2001; 154(5): 1019 - 1030. [Abstract] [Full Text] [PDF]
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H. HAASE, T. PODZUWEIT, G. LUTSCH, A. HOHAUS, S. KOSTKA, C. LINDSCHAU, M. KOTT, R. KRAFT, and I. MORANO Signaling from {beta}-adrenoceptor to L-type calcium channel: identification of a novel cardiac protein kinase A target possessing similarities to AHNAK FASEB J, December 1, 1999; 13(15): 2161 - 2172. [Abstract] [Full Text]
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 F. Sekiya, Y. S. Bae, D. Y. Jhon, S. C. Hwang, and S. G. Rhee AHNAK, a Protein That Binds and Activates Phospholipase C-gamma 1 in the Presence of Arachidonic Acid J. Biol. Chem., May 14, 1999; 274(20): 13900 - 13907. [Abstract] [Full Text] [PDF]
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A. Ishiko, H. Shimizu, T. Masunaga, Y. Kurihara, and T. Nishikawa Detection of Antigens by Immunofluorescence on Ultrathin Cryosections of Skin J. Histochem. Cytochem., December 1, 1998; 46(12): 1455 - 1460. [Abstract] [Full Text]
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B. J. Gentil, C. Delphin, G. O. Mbele, J. C. Deloulme, M. Ferro, J. Garin, and J. Baudier The Giant Protein AHNAK Is a Specific Target for the Calcium- and Zinc-binding S100B Protein. POTENTIAL IMPLICATIONS FOR Ca2+ HOMEOSTASIS REGULATION BY S100B J. Biol. Chem., June 22, 2001; 276(26): 23253 - 23261. [Abstract] [Full Text] [PDF]
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