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Journal of Cell Science, Vol 105, Issue 2 417-422, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
JA Chambers and A Harris
Paediatric Molecular Genetics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.
The main pathology of cystic fibrosis results from obstruction of ducts in several organs by mucous secretions. The cause of this obstruction remains unclear. We have examined expression of the cystic fibrosis transmembrane conductance regulator (CFTR) and of the major pancreatic mucin, MUC1, in primary pancreatic duct and vas deferens epithelial cells, and in pancreatic duct cell lines. MUC1 is expressed at a high level in the primary ductal epithelial cells and at variable levels in different pancreatic adenocarcinoma cell lines. However, although the pancreatic duct is one of the sites in vivo where CFTR transcription is at its highest level, the majority of cell lines examined no longer express CFTR. Only one pancreatic duct cell line, Capan 1, expresses CFTR at a significant level; further, the level of expression is dependent on confluency. We have shown that salt stress alone is not sufficient to account for the build-up of mucous secretions in CF ducts.
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  S.-H. Leir, S. Parry, T. Palmai-Pallag, J. Evans, H. R. Morris, A. Dell, and A. Harris Mucin Glycosylation and Sulphation in Airway Epithelial Cells Is Not Influenced by Cystic Fibrosis Transmembrane Conductance Regulator Expression Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 453 - 461. [Abstract] [Full Text] [PDF]
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 P. Fong, B. E. Argent, W. B. Guggino, and M. A. Gray Characterization of vectorial chloride transport pathways in the human pancreatic duct adenocarcinoma cell line HPAF Am J Physiol Cell Physiol, August 1, 2003; 285(2): C433 - C445. [Abstract] [Full Text] [PDF]
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 B Burghardt, M-L Elkjaer, T-H Kwon, G Z Racz, G Varga, M C Steward, and S Nielsen Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas Gut, July 1, 2003; 52(7): 1008 - 1016. [Abstract] [Full Text]
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 N. Mouchel, F. Broackes-Carter, and A. Harris Alternative 5' exons of the CFTR gene show developmental regulation Hum. Mol. Genet., April 1, 2003; 12(7): 759 - 769. [Abstract] [Full Text] [PDF]
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 D.-H. Hong, G. Petrovics, W. B. Anderson, J. Forstner, and G. Forstner Induction of mucin gene expression in human colonic cell lines by PMA is dependent on PKC-epsilon Am J Physiol Gastrointest Liver Physiol, November 1, 1999; 277(5): G1041 - G1047. [Abstract] [Full Text] [PDF]
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H. Shumaker, H. Amlal, R. Frizzell, C. D. Ulrich II, and M. Soleimani CFTR drives Na+-nHCO-3 cotransport in pancreatic duct cells: a basis for defective HCO-3 secretion in CF Am J Physiol Cell Physiol, January 1, 1999; 276(1): C16 - C25. [Abstract] [Full Text] [PDF]
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 E. B. Mallow, A. Harris, N. Salzman, J. P. Russell, R. J. DeBerardinis, E. Ruchelli, and C. L. Bevins Human Enteric Defensins J. Biol. Chem., February 23, 1996; 271(8): 4038 - 4045. [Abstract] [Full Text] [PDF]
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