spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chambers, J. A.
Right arrow Articles by Harris, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chambers, J. A.
Right arrow Articles by Harris, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 105, Issue 2 417-422, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells

JA Chambers and A Harris
Paediatric Molecular Genetics, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, UK.

The main pathology of cystic fibrosis results from obstruction of ducts in several organs by mucous secretions. The cause of this obstruction remains unclear. We have examined expression of the cystic fibrosis transmembrane conductance regulator (CFTR) and of the major pancreatic mucin, MUC1, in primary pancreatic duct and vas deferens epithelial cells, and in pancreatic duct cell lines. MUC1 is expressed at a high level in the primary ductal epithelial cells and at variable levels in different pancreatic adenocarcinoma cell lines. However, although the pancreatic duct is one of the sites in vivo where CFTR transcription is at its highest level, the majority of cell lines examined no longer express CFTR. Only one pancreatic duct cell line, Capan 1, expresses CFTR at a significant level; further, the level of expression is dependent on confluency. We have shown that salt stress alone is not sufficient to account for the build-up of mucous secretions in CF ducts.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S.-H. Leir, S. Parry, T. Palmai-Pallag, J. Evans, H. R. Morris, A. Dell, and A. Harris
Mucin Glycosylation and Sulphation in Airway Epithelial Cells Is Not Influenced by Cystic Fibrosis Transmembrane Conductance Regulator Expression
Am. J. Respir. Cell Mol. Biol., May 1, 2005; 32(5): 453 - 461.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. Fong, B. E. Argent, W. B. Guggino, and M. A. Gray
Characterization of vectorial chloride transport pathways in the human pancreatic duct adenocarcinoma cell line HPAF
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C433 - C445.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
B Burghardt, M-L Elkjaer, T-H Kwon, G Z Racz, G Varga, M C Steward, and S Nielsen
Distribution of aquaporin water channels AQP1 and AQP5 in the ductal system of the human pancreas
Gut, July 1, 2003; 52(7): 1008 - 1016.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
N. Mouchel, F. Broackes-Carter, and A. Harris
Alternative 5' exons of the CFTR gene show developmental regulation
Hum. Mol. Genet., April 1, 2003; 12(7): 759 - 769.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
D.-H. Hong, G. Petrovics, W. B. Anderson, J. Forstner, and G. Forstner
Induction of mucin gene expression in human colonic cell lines by PMA is dependent on PKC-epsilon
Am J Physiol Gastrointest Liver Physiol, November 1, 1999; 277(5): G1041 - G1047.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Shumaker, H. Amlal, R. Frizzell, C. D. Ulrich II, and M. Soleimani
CFTR drives Na+-nHCO-3 cotransport in pancreatic duct cells: a basis for defective HCO-3 secretion in CF
Am J Physiol Cell Physiol, January 1, 1999; 276(1): C16 - C25.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. B. Mallow, A. Harris, N. Salzman, J. P. Russell, R. J. DeBerardinis, E. Ruchelli, and C. L. Bevins
Human Enteric Defensins
J. Biol. Chem., February 23, 1996; 271(8): 4038 - 4045.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1993