|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 105, Issue 2 461-472, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
MD Peterson, WM Bement and MS Mooseker
Department Cell Biology, Yale University, New Haven, CT 06511-8112.
In the companion paper (M. D. Peterson and M. S. Mooseker (1993). J. Cell Sci. 105, 445-460) we describe a method for modeling brush border assembly in the Caco-2BBe clones. In this study we have examined the molecular changes accompanying cell contact-induced brush border assembly. A subset of brush border proteins was tracked throughout brush border assembly by immunoblotting and by immunofluorescent localization using laser scanning confocal microscopy. Actin, fodrin, villin and presumptive unconventional myosin immunogens were distributed at the periphery of depolarized cells. All proteins partitioned primarily with the membrane fraction upon differential sedimentation of depolarized cell lysates; the fractionation patterns were comparable to those of confluent cells. After a monolayer had formed, each protein showed a redistribution to the apical domain in a discrete sequence. Actin and villin began to shift apically at 2 d, while fodrin and the unconventional myosin immunogens did not redistribute until 3 d. Enterocyte-like localization was observed by 5 d for all proteins. Sucrase-isomaltase was not reliably detectable until 9 d by immunofluorescence, after brush border assembly was complete. Quantitative immunoblot analysis of total cell extracts demonstrated an average 10-fold increase in villin levels, while fodrin levels appeared to remain unchanged. Three putative unconventional myosin immunogens of 140 kDa, 130 kDa, and 110 kDa have been detected previously in the C2BBe cells with a head-specific monoclonal antibody to avian brush border myosin I (M. D. Peterson and M. S. Mooseker (1992) J. Cell Sci. 102, 581-600). Each of these immunogens displayed distinct expression patterns during brush border assembly. The 140 kDa species decreased by half, while the 130 kDa immunogen(s) did not change in any consistent fashion. The 110 kDa protein, presumed to be human brush border myosin I, rose on average 8-fold. A ribonuclease protection assay was also performed using a probe for human brush border myosin I. Equal amounts of total RNA from depolarized and confluent cells were assayed; the level of protected product was approximately 9-fold greater in the confluent cells. The expression patterns of the brush border proteins, coupled with the correlation to the ultrastructural features during brush border assembly in C2BBe cells, show that differentiation of the C2BBe cells closely resembles the changes that occur during human fetal intestinal differentiation.
This article has been cited by other articles:
|
|
 |
|
  E.-Y. Kim, S.-K. Ham, M. K. Shigenaga, and O. Han Bioactive Dietary Polyphenolic Compounds Reduce Nonheme Iron Transport across Human Intestinal Cell Monolayers J. Nutr., September 1, 2008; 138(9): 1647 - 1651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. Walsh, D. R. Ampasala, J. Hatfield, R. Vander Heide, S. Suer, A. K. Rishi, and M. D. Basson Transforming Growth Factor-{beta} Stimulates Intestinal Epithelial Focal Adhesion Kinase Synthesis via Smad- and p38-Dependent Mechanisms Am. J. Pathol., August 1, 2008; 173(2): 385 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Chaturvedi, H. M. Marsh, X. Shang, Y. Zheng, and M. D. Basson Repetitive Deformation Activates Focal Adhesion Kinase and ERK Mitogenic Signals in Human Caco-2 Intestinal Epithelial Cells through Src and Rac1 J. Biol. Chem., January 5, 2007; 282(1): 14 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. V. Graham, F. Wang, D. R. Clayburgh, J. X. Cheng, B. Yoon, Y. Wang, A. Lin, and J. R. Turner Tumor Necrosis Factor-induced Long Myosin Light Chain Kinase Transcription Is Regulated by Differentiation-dependent Signaling Events: CHARACTERIZATION OF THE HUMAN LONG MYOSIN LIGHT CHAIN KINASE PROMOTER J. Biol. Chem., September 8, 2006; 281(36): 26205 - 26215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Basson, M. A. Sanders, R. Gomez, J. Hatfield, R. VanderHeide, V. Thamilselvan, J. Zhang, and M. F. Walsh Focal adhesion kinase protein levels in gut epithelial motility. Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G491 - G499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pozner-Moulis, D. J. Pappas, and D. L. Rimm Met, the Hepatocyte Growth Factor Receptor, Localizes to the Nucleus in Cells at Low Density Cancer Res., August 15, 2006; 66(16): 7976 - 7982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Sanders and M. D. Basson p130cas but Not Paxillin Is Essential for Caco-2 Intestinal Epithelial Cell Spreading and Migration on Collagen IV J. Biol. Chem., June 24, 2005; 280(25): 23516 - 23522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Sanders and M. D. Basson Collagen IV regulates Caco-2 migration and ERK activation via {alpha}1{beta}1- and {alpha}2{beta}1-integrin-dependent Src kinase activation Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G547 - G557. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Houle, W. Li, R. K. Montgomery, and R. J. Grand mRNA localization in polarized intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, April 1, 2003; 284(4): G722 - G727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V Lievin-Le Moal, R Amsellem, A L Servin, and M-H Coconnier Lactobacillus acidophilus (strain LB) from the resident adult human gastrointestinal microflora exerts activity against brush border damage promoted by a diarrhoeagenic Escherichia coli in human enterocyte-like cells Gut, June 1, 2002; 50(6): 803 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Han and M. Wessling-Resnick Copper repletion enhances apical iron uptake and transepithelial iron transport by Caco-2 cells Am J Physiol Gastrointest Liver Physiol, March 1, 2002; 282(3): G527 - G533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Laprise, P. Chailler, M. Houde, J.-F. Beaulieu, M.-J. Boucher, and N. Rivard Phosphatidylinositol 3-Kinase Controls Human Intestinal Epithelial Cell Differentiation by Promoting Adherens Junction Assembly and p38 MAPK Activation J. Biol. Chem., March 1, 2002; 277(10): 8226 - 8234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Peiffer, J. Guignot, A. Barbat, C. Carnoy, S. L. Moseley, B. J. Nowicki, A. L. Servin, and M.-F. Bernet-Camard Structural and Functional Lesions in Brush Border of Human Polarized Intestinal Caco-2/TC7 Cells Infected by Members of the Afa/Dr Diffusely Adhering Family of Escherichia coli Infect. Immun., October 1, 2000; 68(10): 5979 - 5990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Aliaga, C. Deschenes, J.-F. Beaulieu, E. L. Calvo, and N. Rivard Requirement of the MAP kinase cascade for cell cycle progression and differentiation of human intestinal cells Am J Physiol Gastrointest Liver Physiol, September 1, 1999; 277(3): G631 - G641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Turner, J. M. Angle, E. D. Black, J. L. Joyal, D. B. Sacks, and J. L. Madara PKC-dependent regulation of transepithelial resistance: roles of MLC and MLC kinase Am J Physiol Cell Physiol, September 1, 1999; 277(3): C554 - C562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Cohen, I Ophir, and Y. Shaul Induced differentiation in HT29, a human colon adenocarcinoma cell line J. Cell Sci., January 8, 1999; 112(16): 2657 - 2666. [Abstract] [PDF]
|
|
|
|
|
|
R. K. Rao, R. D. Baker, S. S. Baker, A. Gupta, and M. Holycross Oxidant-induced disruption of intestinal epithelial barrier function: role of protein tyrosine phosphorylation Am J Physiol Gastrointest Liver Physiol, October 1, 1997; 273(4): G812 - G823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J.I. Salas, M. L. Rodriguez, A. L. Viciana, D. E. Vega-Salas, and H.-P. Hauri The Apical Submembrane Cytoskeleton Participates in the Organization of the Apical Pole in Epithelial Cells J. Cell Biol., April 21, 1997; 137(2): 359 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Turner, W. I. Lencer, S. Carlson, and J. L. Madara Carboxyl-terminal Vesicular Stomatitis Virus G Protein-tagged Intestinal Na[IMAGE]-dependent Glucose Cotransporter (SGLT1) J. Biol. Chem., March 29, 1996; 271(13): 7738 - 7744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Peterson and M. Mooseker An in vitro model for the analysis of intestinal brush border assembly. I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells J. Cell Sci., January 6, 1993; 105(2): 445 - 460. [Abstract] [PDF]
|
|
|
|
|
|
M. A. Sanders and M. D. Basson Collagen IV-dependent ERK Activation in Human Caco-2 Intestinal Epithelial Cells Requires Focal Adhesion Kinase J. Biol. Chem., November 22, 2000; 275(48): 38040 - 38047. [Abstract] [Full Text] [PDF]
|
|
