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Journal of Cell Science, Vol 105, Issue 2 529-531, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

CO2 production in cell-free extracts of fission yeast detects cell cycle changes

B Novak, A Sveiczer and JM Mitchison
Institute of Cell, Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, Scotland.

CO2 production was followed by manometry in starved cell-free extracts of fission yeast stimulated by unstarved cell-free extracts from a synchronous culture. The degree of stimulus, measured by the lag time in CO2 production, varied markedly during the cell cycle, with a maximum for cells at about mitosis and a minimum for septated cells. Similar differences in lag time were found with unstarved extracts of cdc13.117 grown at 37 degrees C and 35 degrees C.




© The Company of Biologists Ltd 1993