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Journal of Cell Science, Vol 105, Issue 2 551-561, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Transitions between in situ and isolated chromatin

PJ Giannasca, RA Horowitz and CL Woodcock
Department of Biology, University of Massachusetts, Amherst 01003.

We show that the mechanism by which chromatin displaying higher-order structure is usually isolated from nuclei involves a transition to an extended nucleosomal arrangement. After being released from nuclei, chromatin must refold in order to produce the typical chromatin fibers observed in solution. For starfish sperm chromatin with a long nucleosome repeat (222 bp), isolated fibers are significantly wider than those in the nucleus, indicating that the refolding process does not regenerate the native higher-order structure. We also propose that for typical eukaryotic nuclei, the concept that the native state of the (inactive) bulk of the genome is a chromatin fiber with defined architecture be reconsidered.
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© The Company of Biologists Ltd 1993