spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Giannasca, P. J.
Right arrow Articles by Woodcock, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Giannasca, P. J.
Right arrow Articles by Woodcock, C. L.

Journal of Cell Science, Vol 105, Issue 2 551-561, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Transitions between in situ and isolated chromatin

PJ Giannasca, RA Horowitz and CL Woodcock
Department of Biology, University of Massachusetts, Amherst 01003.

We show that the mechanism by which chromatin displaying higher-order structure is usually isolated from nuclei involves a transition to an extended nucleosomal arrangement. After being released from nuclei, chromatin must refold in order to produce the typical chromatin fibers observed in solution. For starfish sperm chromatin with a long nucleosome repeat (222 bp), isolated fibers are significantly wider than those in the nucleus, indicating that the refolding process does not regenerate the native higher-order structure. We also propose that for typical eukaryotic nuclei, the concept that the native state of the (inactive) bulk of the genome is a chromatin fiber with defined architecture be reconsidered.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. F. Kepert, J. Mazurkiewicz, G. L. Heuvelman, K. F. Toth, and K. Rippe
NAP1 Modulates Binding of Linker Histone H1 to Chromatin and Induces an Extended Chromatin Fiber Conformation
J. Biol. Chem., October 7, 2005; 280(40): 34063 - 34072.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. v. Holde and J. Zlatanova
Chromatin Higher Order Structure: Chasing a Mirage?
J. Biol. Chem., April 14, 1995; 270(15): 8373 - 8376.
[Full Text] [PDF]


© The Company of Biologists Ltd 1993