Journal of Cell Science, Vol 105, Issue 3 777-785, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Identification and characterization of a cDNA encoding mouse CAP: a homolog of the yeast adenylyl cyclase associated protein

AB Vojtek and JA Cooper
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

CAP, an adenylyl cyclase associated protein, is present in Saccharomyces cerevisiae and Schizosaccharomyces pombe. In both organisms, CAP is bifunctional: the N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to respond appropriately to upstream regulatory signals, such as RAS in S. cerevisiae; the C-terminal domain is required for cellular morphogenesis. Here, we describe the isolation of a cDNA encoding a CAP homolog from a higher eukaryote. The mouse CAP cDNA contains an open reading frame capable of encoding a 474 amino acid protein. The protein encoded by the mouse CAP cDNA shows extensive homology to the yeast CAP proteins, particularly in the central poly-proline rich region and in the C-terminal domain. By northern analysis, the CAP message appears to be ubiquitous, but not uniform. By indirect immunofluorescence, ectopically expressed mouse CAP protein is found in the cytoplasm of fibroblasts and, in migrating cells, at the leading edge. Expression of the mouse CAP cDNA in S. cerevisiae complements defects associated with loss of the yeast CAP carboxy-terminal domain. Hence, the function of the CAP carboxy-terminal domain has been conserved from yeast to mouse.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. Wang, G.-H. Zhao, Y.-H. Jia, and X.-M. Du Cloning and Characterization of a CAP Gene Expressed in Gossypium arboreum Fuzzless Mutant Crop Sci., November 24, 2008; 48(6): 2314 - 2320. [Abstract] [Full Text] [PDF]

				M. J. Deeks, C. Rodrigues, S. Dimmock, T. Ketelaar, S. K. Maciver, R. Malho, and P. J. Hussey Arabidopsis CAP1 a key regulator of actin organisation and development J. Cell Sci., August 1, 2007; 120(15): 2609 - 2618. [Abstract] [Full Text] [PDF]

				M. B. Ficklin, S. Zhao, and G. Feng Ubiquilin-1 Regulates Nicotine-induced Up-regulation of Neuronal Nicotinic Acetylcholine Receptors J. Biol. Chem., October 7, 2005; 280(40): 34088 - 34095. [Abstract] [Full Text] [PDF]

				E. Bertling, P. Hotulainen, P. K. Mattila, T. Matilainen, M. Salminen, and P. Lappalainen Cyclase-associated Protein 1 (CAP1) Promotes Cofilin-induced Actin Dynamics in Mammalian Nonmuscle Cells Mol. Biol. Cell, May 1, 2004; 15(5): 2324 - 2334. [Abstract] [Full Text] [PDF]

				A. V. HUBBERSTEY and E. P. MOTTILLO Cyclase-associated proteins: CAPacity for linking signal transduction and actin polymerization FASEB J, April 1, 2002; 16(6): 487 - 499. [Abstract] [Full Text] [PDF]

				R. A. Barrero, M. Umeda, S. Yamamura, and H. Uchimiya Arabidopsis CAP Regulates the Actin Cytoskeleton Necessary for Plant Cell Elongation and Division PLANT CELL, January 1, 2002; 14(1): 149 - 163. [Abstract] [Full Text] [PDF]

				Y.-S. Bahn and P. Sundstrom CAP1, an Adenylate Cyclase-Associated Protein Gene, Regulates Bud-Hypha Transitions, Filamentous Growth, and Cyclic AMP Levels and Is Required for Virulence of Candida albicans J. Bacteriol., May 15, 2001; 183(10): 3211 - 3223. [Abstract] [Full Text]

				N. V. Somia, M. J. Schmitt, D. E. Vetter, D. Van Antwerp, S. F. Heinemann, and I. M. Verma LFG: An anti-apoptotic gene that provides protection from Fas-mediated cell death PNAS, October 26, 1999; 96(22): 12667 - 12672. [Abstract] [Full Text] [PDF]

				A. Noegel, F Rivero, R Albrecht, K. Janssen, J Kohler, C. Parent, and M Schleicher Assessing the role of the ASP56/CAP homologue of Dictyostelium discoideum and the requirements for subcellular localization J. Cell Sci., January 10, 1999; 112(19): 3195 - 3203. [Abstract] [PDF]

				Y. Nishida, F. Shima, H. Sen, Y. Tanaka, C. Yanagihara, Y. Yamawaki-Kataoka, K.-i. Kariya, and T. Kataoka Coiled-coil Interaction of N-terminal 36 Residues of Cyclase-associated Protein with Adenylyl Cyclase Is Sufficient for Its Function in Saccharomyces cerevisiae Ras Pathway J. Biol. Chem., October 23, 1998; 273(43): 28019 - 28024. [Abstract] [Full Text] [PDF]

				D. Chantry, A. Vojtek, A. Kashishian, D. A. Holtzman, C. Wood, P. W. Gray, J. A. Cooper, and M. F. Hoekstra p110delta , a Novel Phosphatidylinositol 3-Kinase Catalytic Subunit That Associates with p85 and Is Expressed Predominantly in Leukocytes J. Biol. Chem., August 1, 1997; 272(31): 19236 - 19241. [Abstract] [Full Text] [PDF]

				A. Zelicof, V. Protopopov, D. David, X.-Y. Lin, V. Lustgarten, and J. E. Gerst Two Separate Functions Are Encoded by the Carboxyl-terminal Domains of the Yeast Cyclase-associated Protein and Its Mammalian Homologs. DIMERIZATION AND ACTIN BINDING J. Biol. Chem., July 26, 1996; 271(30): 18243 - 18252. [Abstract] [Full Text] [PDF]

				D L Turner and H Weintraub Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate. Genes & Dev., June 15, 1994; 8(12): 1434 - 1447. [Abstract] [PDF]

				G Yu, J Swiston, and D Young Comparison of human CAP and CAP2, homologs of the yeast adenylyl cyclase-associated proteins J. Cell Sci., January 6, 1994; 107(6): 1671 - 1678. [Abstract] [PDF]

				C. Figueroa, J. Taylor, and A. B. Vojtek Prenylated Rab Acceptor Protein Is a Receptor for Prenylated Small GTPases J. Biol. Chem., July 20, 2001; 276(30): 28219 - 28225. [Abstract] [Full Text] [PDF]

				B.-H. Zhang, E. D. Tang, T. Zhu, M. E. Greenberg, A. B. Vojtek, and K.-L. Guan Serum- and Glucocorticoid-inducible Kinase SGK Phosphorylates and Negatively Regulates B-Raf J. Biol. Chem., August 17, 2001; 276(34): 31620 - 31626. [Abstract] [Full Text] [PDF]