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Journal of Cell Science, Vol 105, Issue 4 1095-1100, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
SM Byrne and CS Hoffman
Department of Biology, Boston College, Chestnut Hill, MA 02167.
An important eukaryotic signal transduction pathway involves the regulation of the effector enzyme adenylate cyclase, which produces the second messenger, cAMP. Previous genetic analyses demonstrated that glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene requires the function of adenylate cyclase, encoded by the git2 gene. As mutations in git2 and in six additional git genes are suppressed by exogenous cAMP, these 'upstream' git genes were proposed to act to produce a glucose-induced cAMP signal. We report here that assays of cAMP levels in wild-type and various mutant S. pombe cells, before and after exposure to glucose, show that this is the case. The data suggest that the cAMP signal results from the activation of adenylate cyclase. Therefore these 'upstream' git genes appear to encode a glucose-induced adenylate cyclase activation pathway. Assays of cAMP on a strain carrying a mutation in the git6 gene, which acts downstream of adenylate cyclase, indicate that git6 may function to feedback regulate adenylate cyclase activity. Thus git6 may encode a cAMP-dependent protein kinase.
This article has been cited by other articles:
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K. Hirota, C. S. Hoffman, T. Shibata, and K. Ohta Fission Yeast Tup1-Like Repressors Repress Chromatin Remodeling at the fbp1+ Promoter and the ade6-M26 Recombination Hotspot Genetics, October 1, 2003; 165(2): 505 - 515. [Abstract] [Full Text] [PDF]
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S. Landry and C. S. Hoffman The git5 G{beta} and git11 G{{gamma}} Form an Atypical G{beta}{{gamma}} Dimer Acting in the Fission Yeast Glucose/cAMP Pathway Genetics, March 1, 2001; 157(3): 1159 - 1168. [Abstract] [Full Text]
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R. T. K. Janoo, L. A. Neely, B. R. Braun, S. K. Whitehall, and C. S. Hoffman Transcriptional Regulators of the Schizosaccharomyces pombe fbp1 Gene Include Two Redundant Tup1p-like Corepressors and the CCAAT Binding Factor Activation Complex Genetics, March 1, 2001; 157(3): 1205 - 1215. [Abstract] [Full Text]
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R. M. Welton and C. S. Hoffman Glucose Monitoring in Fission Yeast via the gpa2 G{alpha}, the git5 G{beta} and the git3 Putative Glucose Receptor Genetics, October 1, 2000; 156(2): 513 - 521. [Abstract] [Full Text]
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S. Landry, M. T. Pettit, E. Apolinario, and C. S. Hoffman The Fission Yeast git5 Gene Encodes a G{beta} Subunit Required for Glucose-Triggered Adenylate Cyclase Activation Genetics, April 1, 2000; 154(4): 1463 - 1471. [Abstract] [Full Text]
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J. M. Gancedo Yeast Carbon Catabolite Repression Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 334 - 361. [Abstract] [Full Text] [PDF]
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