|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 105, Issue 4 913-921, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
TR Cheek, A Morgan, AJ O'Sullivan, RB Moreton, MJ Berridge and RD Burgoyne
Department of Zoology, University of Cambridge, UK.
The spatial organization of agonist-induced Ca2+ entry in single bovine adrenal chromaffin cells has been investigated using video-imaging techniques to visualize fura-2 quenching by the Ca2+ surrogate, Mn2+. The potent secretagogue histamine, in addition to releasing Ca2+ from intracellular stores, resulted in a large influx of external Mn2+ that occurred over the entire surface of the cell. The influx of Ca2+ that this mirrors was found to be an obligatory requirement for the triggering of catecholamine release by histamine, which suggests that such a global influx of Ca2+ into the cell probably underlies the ability of this agonist to stimulate a large secretory response. By contrast, the weaker secretagogue angiotensin II, which also acts through the second messenger inositol trisphosphate, produced a localized entry of external Mn2+ in 64% of cells. In these cells, localized Mn2+ entry always occurred at the pole of the cell in which the angiotensin II-induced rise in [Ca2+]i was largest. Since exocytosis in response to angiotensin II has previously been shown to be restricted to this same pole of the cell (Cheek et al. (1989). J. Cell Biol. 109, 1219-1227), these results suggest that localized influx of Ca2+ in response to angiotensin II could underlie the polarized exocytotic response observed with this stimulus. These results directly demonstrate that different agonists can induce different patterns of divalent cation influx in the same cells and, furthermore, suggest how these different patterns can have a direct influence on cellular function.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Matsuda, Y. Toyohira, S. Ueno, M. Tsutsui, and N. Yanagihara Simvastatin Inhibits Catecholamine Secretion and Synthesis Induced by Acetylcholine via Blocking Na+ and Ca2+ Influx in Bovine Adrenal Medullary Cells J. Pharmacol. Exp. Ther., October 1, 2008; 327(1): 130 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Garcia, A. M. Garcia-De-Diego, L. Gandia, R. Borges, and J. Garcia-Sancho Calcium signaling and exocytosis in adrenal chromaffin cells. Physiol Rev, October 1, 2006; 86(4): 1093 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Ennion, A. D. Powell, and E. P. Seward Identification of the P2Y12 Receptor in Nucleotide Inhibition of Exocytosis from Bovine Chromaffin Cells Mol. Pharmacol., September 1, 2004; 66(3): 601 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Burgoyne and A. Morgan Secretory Granule Exocytosis Physiol Rev, April 1, 2003; 83(2): 581 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. G. Obukhov and M. C. Nowycky TRPC4 Can Be Activated by G-protein-coupled Receptors and Provides Sufficient Ca2+ to Trigger Exocytosis in Neuroendocrine Cells J. Biol. Chem., May 3, 2002; 277(18): 16172 - 16178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J Wallace, C. Chen, and P. D Marley Histamine promotes excitability in bovine adrenal chromaffin cells by inhibiting an M-current J. Physiol., May 1, 2002; 540(3): 921 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Pan, A. Jeromin, K. Lundstrom, S. H. Yoo, J. Roder, and A. P. Fox Alterations in Exocytosis Induced by Neuronal Ca2+ Sensor-1 in Bovine Chromaffin Cells J. Neurosci., April 1, 2002; 22(7): 2427 - 2433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Teschemacher and E. P. Seward Bidirectional Modulation of Exocytosis by Angiotensin II Involves Multiple G-Protein-Regulated Transduction Pathways in Chromaffin Cells J. Neurosci., July 1, 2000; 20(13): 4776 - 4785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. P. M. Currie and A. P. Fox Voltage-Dependent, Pertussis Toxin Insensitive Inhibition of Calcium Currents by Histamine in Bovine Adrenal Chromaffin Cells J Neurophysiol, March 1, 2000; 83(3): 1435 - 1442. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Barry and T. Cheek Extracellular ATP triggers two functionally distinct calcium signalling pathways in PC12 cells J. Cell Sci., January 2, 1994; 107(2): 451 - 462. [Abstract] [PDF]
|
|
|
|
|
|
D. J Wallace, C. Chen, and P. D Marley Histamine promotes excitability in bovine adrenal chromaffin cells by inhibiting an M-current J. Physiol., May 1, 2002; 540(3): 921 - 939. [Abstract] [Full Text] [PDF]
|
|
