Journal of Cell Science, Vol 106, Issue 1 135-143, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

The expression of basic fibroblast growth factor and its receptor in cell lines derived from normal human mammary gland and a benign mammary lesion

Y Ke, DG Fernig, MC Wilkinson, JH Winstanley, JA Smith, PS Rudland and R Barraclough
Department of Biochemistry, University of Liverpool, UK.

mRNA for basic Fibroblast Growth Factor (bFGF) was expressed in a series of SV40-transformed human mammary cell lines as molecules of 7.1, 3.6, 2.0 and 1.2 kb. This expression was much weaker in those lines of epithelial morphology than in myoepithelial-like cell lines derived from them. It was confirmed, using northern hybridization to single-stranded RNA probes, that the multiple mRNAs were transcribed from the coding strand for bFGF. bFGF activity was detected in extracts of the cells and the relative amounts of activity corresponded in general to the amounts of mRNA found. Similar results were obtained from spontaneously transformed cell lines derived from a human benign breast lesion. The presence of bFGF protein in the extracts was confirmed by western blotting, which showed a band of 18-19 kDa, migrating in the same position as authentic bFGF; in addition, the myoepithelial-like cells showed prominent bands of bFGF at 24 and 26 kDa. No FGF receptor was detectable by the binding of 125I-bFGF to the SV40-transformed cell lines or to the epithelial cell lines from the benign breast lesion, but both high- and low-affinity receptors were found on myoepithelial-like cells derived from the latter. The results indicate that differentiation to the human myoepithelial-like phenotype in culture is associated with the enhanced expression of bFGF, and it is suggested that bFGF, immunocytochemically detected in the basement membrane of the human breast, may arise, at least in part, from the myoepithelial cells of the mammary parenchyma.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				D. Liu, P. S. Rudland, D. R. Sibson, A. Platt-Higgins, and R. Barraclough Human Homologue of Cement Gland Protein, a Novel Metastasis Inducer Associated with Breast Carcinomas Cancer Res., May 1, 2005; 65(9): 3796 - 3805. [Abstract] [Full Text] [PDF]

				C. Jing, M. A. El-Ghany, C. Beesley, C. S. Foster, P. S. Rudland, P. Smith, and Y. Ke Tazarotene-Induced Gene 1 (TIG1) Expression in Prostate Carcinomas and Its Relationship to Tumorigenicity J Natl Cancer Inst, April 3, 2002; 94(7): 482 - 490. [Abstract] [Full Text] [PDF]

				C. Jing, C. Beesley, C. S. Foster, H. Chen, P. S. Rudland, D. C. West, H. Fujii, P. H. Smith, and Y. Ke Human Cutaneous Fatty Acid-binding Protein Induces Metastasis by Up-Regulating the Expression of Vascular Endothelial Growth Factor Gene in Rat Rama 37 Model Cells Cancer Res., June 1, 2001; 61(11): 4357 - 4364. [Abstract] [Full Text] [PDF]

				C. Jing, C. Beesley, C. S. Foster, P. S. Rudland, H. Fujii, T. Ono, H. Chen, P. H. Smith, and Y. Ke Identification of the Messenger RNA for Human Cutaneous Fatty Acid-binding Protein as a Metastasis Inducer Cancer Res., May 1, 2000; 60(9): 2390 - 2398. [Abstract] [Full Text]

				H. Rahmoune, H.-L. Chen, J. T. Gallagher, P. S. Rudland, and D. G. Fernig Interaction of Heparan Sulfate from Mammary Cells with Acidic Fibroblast Growth Factor (FGF) and Basic FGF. REGULATION OF THE ACTIVITY OF BASIC FGF BY HIGH AND LOW AFFINITY BINDING SITES IN HEPARAN SULFATE J. Biol. Chem., March 27, 1998; 273(13): 7303 - 7310. [Abstract] [Full Text] [PDF]

				V. Nurcombe, C. E. Smart, H. Chipperfield, S. M. Cool, B. Boilly, and H. Hondermarck The Proliferative and Migratory Activities of Breast Cancer Cells Can Be Differentially Regulated by Heparan Sulfates J. Biol. Chem., September 22, 2000; 275(39): 30009 - 30018. [Abstract] [Full Text] [PDF]