|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 106, Issue 1 389-400, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
P Joshi, CY Chung, I Aukhil and HP Erickson
Department of Cell Biology, Duke University Medical Center, Durham, NC 27710.
We have found that endothelial cells adhere much more strongly than fibroblasts to domains of tenascin and fibronectin. Endothelial cells adhered weakly, without spreading, to bacterial expression proteins corresponding to the tenth fibronectin type III (FN-III) domain of fibronectin, which contains the RGD. A larger fibronectin protein, containing this domain and the three amino-terminal 'synergy' domains gave strong adhesion and spreading. Two widely separated domains of tenascin gave adhesion. The third FN-III domain, TNfn3, which contains an RGD sequence in human and chicken tenascin, gave very strong adhesion and spreading of endothelial cells when tested as an isolated domain. Larger segments containing TNfn3 and the adjacent TNfn2 gave weaker adhesion, probably because the RGD sequence is partially blocked. Adhesion to this domain required divalent cations, was exquisitely sensitive to soluble GRGDSP peptide, and was blocked by antisera to the integrin alpha v beta 3. The second tenascin adhesion domain was the fibrinogen-like C-terminal knob, TNfbg. Cells adhered to but did not spread on this domain. This adhesion required divalent cations and was also sensitive to GRGDSP peptide, so it may be mediated by an integrin receptor. We have explored a range of conditions for preparing the adhesion substratum, and our results may resolve the controversy over whether tenascin can act as a substratum adhesion molecule. When coated for short times (1-2 hours) on plastic, tenascin had no adhesion activity, in contrast to fibronectin and the expression proteins, which gave strong adhesion under these conditions. When coated for longer times (12-24 hours) on plastic, the tenascin substratum supported good adhesion, but not spreading, of endothelial cells. Tenascin coated on nitrocellulose gave substantially stronger adhesion than on plastic, but still required long coating times for maximal activity. Adhesion of endothelial cells to native TN was inhibited by GRDGSP peptide. The cell adhesion activity demonstrates the presence on endothelial cells of tenascin receptors, which may play a supportive role in angiogenesis, in the structure of blood vessels, or in binding tenascin to the cell surface to elicit or enhance a signalling function.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Saito, H. Imazeki, S. Miura, T. Yoshimura, H. Okutsu, Y. Harada, T. Ohwaki, O. Nagao, S. Kamiya, R. Hayashi, et al. A Peptide Derived from Tenascin-C Induces 1 Integrin Activation through Syndecan-4 J. Biol. Chem., November 30, 2007; 282(48): 34929 - 34937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Ng, K. S. Billings, T. Ohashi, M. D. Allen, R. B. Best, L. G. Randles, H. P. Erickson, and J. Clarke Designing an extracellular matrix protein with enhanced mechanical stability PNAS, June 5, 2007; 104(23): 9633 - 9637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Jang, J.-H. Hwang, C.-P. Chung, and P.-H. Choung Identification and Kinetics Analysis of a Novel Heparin-binding Site (KEDK) in Human Tenascin-C J. Biol. Chem., June 11, 2004; 279(24): 25562 - 25566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. T. Mercado, A. Nur-e-Kamal, H.-Y. Liu, S. R. Gross, R. Movahed, and S. Meiners Neurite Outgrowth by the Alternatively Spliced Region of Human Tenascin-C Is Mediated by Neuronal {alpha}7{beta}1 Integrin J. Neurosci., January 7, 2004; 24(1): 238 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Leyh, M. Wilhelmi, T. Walles, K. Kallenbach, P. Rebe, A. Oberbeck, T. Herden, A. Haverich, and H. Mertsching Acellularized porcine heart valve scaffolds for heart valve tissue engineering and the risk of cross-species transmission of porcine endogenous retrovirus J. Thorac. Cardiovasc. Surg., October 1, 2003; 126(4): 1000 - 1004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Filenius, T. Tervo, and I. Virtanen Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3317 - 3325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Leyh, M. Wilhelmi, P. Rebe, S. Fischer, T. Kofidis, A. Haverich, and H. Mertsching In vivo repopulation of xenogeneic and allogeneic acellular valve matrix conduits in the pulmonary circulation Ann. Thorac. Surg., May 1, 2003; 75(5): 1457 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zagzag, B. Shiff, G. I. Jallo, M. A. Greco, C. Blanco, H. Cohen, J. Hukin, J. C. Allen, and D. R. Friedlander Tenascin-C Promotes Microvascular Cell Migration and Phosphorylation of Focal Adhesion Kinase Cancer Res., May 1, 2002; 62(9): 2660 - 2668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Ljubimov, M. Saghizadeh, R. Pytela, D. Sheppard, and M. C. Kenney Increased Expression of Tenascin-C-binding Epithelial Integrins in Human Bullous Keratopathy Corneas J. Histochem. Cytochem., November 1, 2001; 49(11): 1341 - 1350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Wenk, K. S. Midwood, and J. E. Schwarzbauer Tenascin-C Suppresses Rho Activation J. Cell Biol., August 21, 2000; 150(4): 913 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schenk, R. Chiquet-Ehrismann, and E. J. Battegay The Fibrinogen Globe of Tenascin-C Promotes Basic Fibroblast Growth Factor-induced Endothelial Cell Elongation Mol. Biol. Cell, September 1, 1999; 10(9): 2933 - 2943. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Gibson, D. I. Leavesley, and L. K. Ashman Microfibril-associated Glycoprotein-2 Specifically Interacts with a Range of Bovine and Human Cell Types via alpha Vbeta 3 Integrin J. Biol. Chem., May 7, 1999; 274(19): 13060 - 13065. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhao, S. L. Young, and J. C. McIntosh Induction of tenascin in rat lungs undergoing bleomycin-induced pulmonary fibrosis Am J Physiol Lung Cell Mol Physiol, June 1, 1998; 274(6): L1049 - L1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yokosaki, N. Matsuura, S. Higashiyama, I. Murakami, M. Obara, M. Yamakido, N. Shigeto, J. Chen, and D. Sheppard Identification of the Ligand Binding Site for the Integrin alpha 9beta 1 in the Third Fibronectin Type III Repeat of Tenascin-C J. Biol. Chem., May 8, 1998; 273(19): 11423 - 11428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Phillips, L. Krushel, and K. Crossin Domains of tenascin involved in glioma migration J. Cell Sci., January 4, 1998; 111(8): 1095 - 1104. [Abstract] [PDF]
|
|
|
|
|
|
D. W. LaFleur, J. Chiang, J. A. Fagin, S. M. Schwartz, P. K. Shah, K. Wallner, J. S. Forrester, and B. G. Sharifi Aortic Smooth Muscle Cells Interact with Tenascin-C through Its Fibrinogen-like Domain J. Biol. Chem., December 26, 1997; 272(52): 32798 - 32803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Fischer, R. P. Tucker, R. Chiquet-Ehrismann, and J. C. Adams Cell-Adhesive Responses to Tenascin-C Splice Variants Involve Formation of Fascin Microspikes Mol. Biol. Cell, October 1, 1997; 8(10): 2055 - 2075. [Abstract] [Full Text]
|
|
|
|
|
|
F. Elefteriou, J.-Y. Exposito, R. Garrone, and C. Lethias Characterization of the Bovine Tenascin-X J. Biol. Chem., September 5, 1997; 272(36): 22866 - 22874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Maseruka, R E Bonshek, and A B Tullo Tenascin-C expression in normal, inflamed, and scarred human corneas Br J Ophthalmol, August 1, 1997; 81(8): 677 - 682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Milev, D. Fischer, M. Haring, T. Schulthess, R. K. Margolis, R. Chiquet-Ehrismann, and R. U. Margolis The Fibrinogen-like Globe of Tenascin-C Mediates Its Interactions with Neurocan and Phosphacan/Protein-tyrosine Phosphatase-zeta /beta J. Biol. Chem., June 13, 1997; 272(24): 15501 - 15509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Clark, H. P. Erickson, and T. A. Springer Tenascin Supports Lymphocyte Rolling J. Cell Biol., May 5, 1997; 137(3): 755 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Hashimoto-Uoshima, Y. Yan, G Schneider, and I Aukhil The alternatively spliced domains EIIIB and EIIIA of human fibronectin affect cell adhesion and spreading J. Cell Sci., January 9, 1997; 110(18): 2271 - 2280. [Abstract] [PDF]
|
|
|
|
|
|
D Fischer, M Brown-Ludi, T Schulthess, and R Chiquet-Ehrismann Concerted action of tenascin-C domains in cell adhesion, anti-adhesion and promotion of neurite outgrowth J. Cell Sci., January 7, 1997; 110(13): 1513 - 1522. [Abstract] [PDF]
|
|
|
|
|
|
C. Chung and H. Erickson Glycosaminoglycans modulate fibronectin matrix assembly and are essential for matrix incorporation of tenascin-C J. Cell Sci., January 6, 1997; 110(12): 1413 - 1419. [Abstract] [PDF]
|
|
|
|
|
|
E. I. Deryugina and M. A. Bourdon Tenascin mediates human glioma cell migration and modulates cell migration on fibronectin J. Cell Sci., March 1, 1996; 109(3): 643 - 652. [Abstract] [PDF]
|
|
|
|
|
|
J.-P. Girard and T. A. Springer Modulation of Endothelial Cell Adhesion by Hevin, an Acidic Protein Associated with High Endothelial Venules J. Biol. Chem., February 23, 1996; 271(8): 4511 - 4517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Giese, M. Loo, S. Norman, S Treasurywala, and M. Berens Contrasting migratory response of astrocytoma cells to tenascin mediated by different integrins J. Cell Sci., January 8, 1996; 109(8): 2161 - 2168. [Abstract] [PDF]
|
|
|
|
|
|
E. Mackie and S Ramsey Modulation of osteoblast behaviour by tenascin J. Cell Sci., January 6, 1996; 109(6): 1597 - 1604. [Abstract] [PDF]
|
|
|
|
|
|
D. Fischer, R. Chiquet-Ehrismann, C. Bernasconi, and M. Chiquet A Single Heparin Binding Region within the Fibrinogen-like Domain Is Functional in Chick Tenascin-C J. Biol. Chem., February 17, 1995; 270(7): 3378 - 3384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Redick and J. Schwarzbauer Rapid intracellular assembly of tenascin hexabrachions suggests a novel cotranslational process J. Cell Sci., January 4, 1995; 108(4): 1761 - 1769. [Abstract] [PDF]
|
|
|
|
|
|
P. Jones, N Boudreau, C. Myers, H. Erickson, and M. Bissell Tenascin-C inhibits extracellular matrix-dependent gene expression in mammary epithelial cells. Localization of active regions using recombinant tenascin fragments J. Cell Sci., January 2, 1995; 108(2): 519 - 527. [Abstract] [PDF]
|
|
|
|
|
|
K. Yokoyama, H. P. Erickson, Y. Ikeda, and Y. Takada Identification of Amino Acid Sequences in Fibrinogen gamma -Chain and Tenascin C C-terminal Domains Critical for Binding to Integrin alpha vbeta 3 J. Biol. Chem., May 26, 2000; 275(22): 16891 - 16898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lethias, F. Elefteriou, G. Parsiegla, J.-Y. Exposito, and R. Garrone Identification and Characterization of a Conformational Heparin-binding Site Involving Two Fibronectin Type III Modules of Bovine Tenascin-X J. Biol. Chem., May 4, 2001; 276(19): 16432 - 16438. [Abstract] [Full Text] [PDF]
|
|
