|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 106, Issue 2 503-511, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
A Poliard, D Lamblin, PJ Marie, MH Buc-Caron and O Kellermann
Laboratoire de Differenciation Cellulaire de l'Institut Pasteur, Unite Associee du Centre National de la Recherche Scientifique 1148, Paris, France.
The mesodermal clone C1 was derived from the multipotent embryonal carcinoma 1003 cell line transformed with the plasmid pK4 carrying SV40 oncogenes under the control of the adenovirus E1A promoter. We have shown that the C1 clone becomes committed to the osteogenic pathway when cultured in aggregates in the presence of mediators of the osteogenic differentiation. To further validate C1 as a model with which to study osteogenesis in vitro the kinetics of its differentiation was studied, focusing on the histology of the aggregates and on the expression of a set of genes corresponding to representative bone matrix proteins. The presence of ascorbic acid and beta- glycerophosphate specifically leads to mineralization in almost 100% of the aggregates. Transcription of the above genes, silent in exponentially growing cells, specifically occurred with the establishment of cell-cell contacts independently of the presence of ascorbic acid and inorganic phosphate. The latter, however, were absolutely required for matrix deposition and mineralization. In their presence, one observed an overall decline in type I collagen and alkaline phosphatase transcripts while osteocalcin and osteopontin transcripts preferentially accumulated in cells lining the mineralizing foci. Concomitantly, type I collagen and osteocalcin became extracellularly deposited. The osteogenic differentiation of C1 occurred while cells were still proliferating. The C1 clone thus behaves as a mesodermal stem cell, becoming committed to the osteogenic pathway upon: firstly, establishment of cellular contacts; and secondly, addition of ascorbate and beta-glycerophosphate. It therefore appears to be a promising in vitro system for deciphering the molecular basis of osteoblast ontogeny.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  J.-M. Launay, B. Schneider, S. Loric, M. Da Prada, and O. Kellermann Serotonin transport and serotonin transporter-mediated antidepressant recognition are controlled by 5-HT2B receptor signaling in serotonergic neuronal cells FASEB J, September 1, 2006; 20(11): 1843 - 1854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nifuji, O. Kellermann, and M. Noda Noggin Inhibits Chondrogenic But Not Osteogenic Differentiation in Mesodermal Stem Cell Line C1 and Skeletal Cells Endocrinology, July 1, 2004; 145(7): 3434 - 3442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. W. Sun, H. Kobayashi, M. Suzuki, N. Kanayama, and T. Terao Follicle-Stimulating Hormone and Insulin-Like Growth Factor I Synergistically Induce Up-Regulation of Cartilage Link Protein (Crtl1) via Activation of Phosphatidylinositol-Dependent Kinase/Akt in Rat Granulosa Cells Endocrinology, March 1, 2003; 144(3): 793 - 801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Schinke and G. Karsenty Characterization of Osf1, an Osteoblast-specific Transcription Factor Binding to a Critical cis-acting Element in the Mouse Osteocalcin Promoters J. Biol. Chem., October 15, 1999; 274(42): 30182 - 30189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Laoide, Y Courty, I Gastinne, C Thibaut, O Kellermann, and F Rougeon Immortalised mouse submandibular epithelial cell lines retain polarised structural and functional properties J. Cell Sci., January 12, 1996; 109(12): 2789 - 2800. [Abstract] [PDF]
|
|
