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Journal of Cell Science, Vol 106, Issue 2 545-553, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Cloning of the polyubiquitin cDNA from the marine sponge Geodia cydonium and its preferential expression during reaggregation of cells

K Pfeifer, W Frank, HC Schroder, V Gamulin, B Rinkevich, R Batel, IM Muller and WE Muller
Institut fur Physiologische Chemie, Universitat, Mainz, Germany.

Ubiquitination of proteins is a critical step in the controlled degradation process of many polypeptides. Here we show that sponges, the simplest multicellular group of eukaryotic organisms, are also equipped with the ubiquitin pathway. The polyubiquitin cDNA was isolated and characterized from the marine sponge Geodia cydonium. The open reading frame contains six ubiquitin moieties, which are lined up head to tail without spacers. A comparison of the predicted amino acid sequence of the six sponge ubiquitin-coding units with those from other organisms revealed a high degree of homology (> 93%). The ubiquitin gene is expressed to almost the same extent in the two main compartments of the sponge, the cortex and the medulla. However, only in the cortex are detectable amounts of the ubiquitin protein synthesized. The ubiquitin protein isolated from the sponge organism was found to initiate protein degradation in the heterologous reticulocyte system in the same manner as bovine ubiquitin. In vitro studies with dissociated sponge cells revealed that the homologous aggregation factor causes (i) a strong increase in the steady-state level of mRNA coding for ubiquitin and (ii) a drastic increase in ubiquitin protein synthesis, while the homologous lectin failed to display that effect in isolated cells. These data suggest that ubiquitin may play a role in sponge morphogenesis.
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 J. Biol. Chem.Home page
X. Fernandez-Busquets and M. M. Burger
The Main Protein of the Aggregation Factor Responsible for Species-specific Cell Adhesion in the Marine Sponge Microciona prolifera Is Highly Polymorphic
J. Biol. Chem., October 31, 1997; 272(44): 27839 - 27847.
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© The Company of Biologists Ltd 1993