|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 106, Issue 2 591-595, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
G Liu, H Kuwayama, S Ishida and PC Newell
Department of Biochemistry, University of Oxford, UK.
Evidence has previously been reported that, during chemotaxis of the cellular slime mould Dictyostelium discoideum, cyclic GMP regulates the association of myosin II with the cytoskeleton and that this regulation is effected by inhibiting myosin II heavy chain phosphorylation (Liu and Newell, J. Cell Sci., 90, 123-129, 1988; 98, 483-490, 1991). Here we provide further evidence in support of this hypothesis using a mutant (KI-10) that is defective in chemotaxis and lacks the normal cyclic AMP-induced cyclic GMP response. We found that the cyclic AMP-induced cytoskeletal actin response was similar to that of the parental strain in this mutant (although showing a slight displacement in the dose-response curve) but the cytoskeletal myosin II heavy chain response was abolished. Moreover, the mutant showed no phosphorylation of myosin II heavy chain in response to cyclic AMP. Compared to the parental strain XP55, the mutant cells contained approximately 40% more protein and their doubling time was 30% longer. These differences could be due to differences in the efficiency of cell division, a process in which the proper regulation of myosin function is essential and in which cyclic GMP may therefore play a role.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Sato, H. Kuwayama, W. N. van Egmond, A. L. K. Takayama, H. Takagi, P. J. M. van Haastert, T. Yanagida, and M. Ueda Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling PNAS, April 21, 2009; 106(16): 6667 - 6672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Veltman and P. J.M. Van Haastert Guanylyl Cyclase Protein and cGMP Product Independently Control Front and Back of Chemotaxing Dictyostelium Cells Mol. Biol. Cell, September 1, 2006; 17(9): 3921 - 3929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Parent and P. N. Devreotes A Cell's Sense of Direction Science, April 30, 1999; 284(5415): 765 - 770. [Abstract] [Full Text]
|
|
|
|
|
|
L. A. Silveira, J. L. Smith, J. L. Tan, and J. A. Spudich MLCK-A, an unconventional myosin light chain kinase from Dictyostelium, is activated by a cGMP-dependent pathway PNAS, October 27, 1998; 95(22): 13000 - 13005. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kuwayama and P. J.M. VanHaastert Regulation of Guanylyl Cyclase by a cGMP-binding Protein during Chemotaxis in Dictyostelium discoideum J. Biol. Chem., September 27, 1996; 271(39): 23718 - 23724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Oyama and M. Oyama cGMP Accumulation Induced by Hypertonic Stress in Dictyostelium discoideum J. Biol. Chem., March 8, 1996; 271(10): 5574 - 5579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Yumura, K Furuya, and I Takeuchi Intracellular free calcium responses during chemotaxis of Dictyostelium cells J. Cell Sci., January 11, 1996; 109(11): 2673 - 2678. [Abstract] [PDF]
|
|
|
|
 |
|
 R A Firtel Integration of signaling information in controlling cell-fate decisions in Dictyostelium. Genes & Dev., June 15, 1995; 9(12): 1427 - 1444. [PDF]
|
|
|
|
|
|
L. M. Futey, Q. G. Medley, G. P. Côté, and T. T. Egelhoff Structural Analysis of Myosin Heavy Chain Kinase A from Dictyostelium J. Biol. Chem., January 13, 1995; 270(2): 523 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liu and P. C. Newell Regulation of myosin regulatory light chain phosphorylation via cyclic GMP during chemotaxis of Dictyostelium J. Cell Sci., July 1, 1994; 107(7): 1737 - 1743. [Abstract] [PDF]
|
|
