spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fenyves, A. M.
Right arrow Articles by Spanel-Borowski, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fenyves, A. M.
Right arrow Articles by Spanel-Borowski, K.

Journal of Cell Science, Vol 106, Issue 3 879-890, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Cultured microvascular endothelial cells (MVEC) differ in cytoskeleton, expression of cadherins and fibronectin matrix. A study under the influence of interferon-gamma

AM Fenyves, J Behrens and K Spanel-Borowski
Anatomisches Institut, Universitat Basel, Switzerland.

Endothelial cells are known to undergo transitions in cell shape during long-term culture. Thus, the assumption that the separate phenotypes of microvascular endothelial cells (MVEC) recently isolated from bovine corpus luteum represent constitutively different cell strains cannot automatically be made. For this reason, particular morphological qualities from four of five reported MVEC types were studied. Confluent cultures of MVEC types 1, 3, 4 and 5 were either left untreated or exposed to recombinant bovine interferon-gamma (IFN-gamma; 200 units/0.5 ml culture medium) for 3 days. Paraformaldehyde-fixed monolayers were permeabilized with Triton X-100 prior to the detection of filamentous actin, using phalloidin-FITC. Vimentin filaments, cytokeratin filaments, microtubules, E- and N-cadherins as molecules of cell adhesion plaques, and fibronectin filaments were localized by the application of specific antibodies in combination with epifluorescence microscopy. Cells from untreated single cultures uniformly and reproducibly showed an actin cytoskeleton that distinguished the particular MVEC type. MVEC type 1 presented a circular band of fine actin filaments. MVEC type 3 preferentially had developed a starburst-like actin pattern. MVEC type 4 mainly exhibited a polygonal network. MVEC type 5 showed a prominent circular band of thick microfilament bundles from which short filaments radiated. Cytokeratin filaments were noted in MVEC type 1 only. Vimentin filaments occurred as a dense network constricted to the central area in MVEC type 1, while they were spread out in MVEC types 3 and 4. A wavy path comparable to the course of microtubules was apparent in MVEC type 5. Fibronectin assembled into two differently shaped layers at the basal cell side of each MVEC type. Under IFN-gamma treatment, cytoskeletal diversities were maintained between the MVEC types, yet each MVEC type showed specific modulations to its cytoskeleton and to its fibronectin matrix. Upregulation of anti-E-cadherin labelling was detected in MVEC type 1, showing a fluorescent cell border of linear contour. The upregulation of E-cadherin by IFN-gamma treatment could also be demonstrated by western blotting, which revealed a 135 kDa full-sized molecule and a 95 kDa tryptic fragment characteristic of cadherins. Anti-N-cadherin labelling was evident for MVEC type 5, giving rise to a fluorescent punctate cell margin. Our investigations support the existence of truly separate MVEC types.


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M. Utech, A. I. Ivanov, S. N. Samarin, M. Bruewer, J. R. Turner, R. J. Mrsny, C. A. Parkos, and A. Nusrat
Mechanism of IFN-{gamma}-induced Endocytosis of Tight Junction Proteins: Myosin II-dependent Vacuolarization of the Apical Plasma Membrane
Mol. Biol. Cell, October 1, 2005; 16(10): 5040 - 5052.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Kisliouk, H. Podlovni, K. Spanel-Borowski, O. Ovadia, Q.-Y. Zhou, and R. Meidan
Prokineticins (Endocrine Gland-Derived Vascular Endothelial Growth Factor and BV8) in the Bovine Ovary: Expression and Role as Mitogens and Survival Factors for Corpus Luteum-Derived Endothelial Cells
Endocrinology, September 1, 2005; 146(9): 3950 - 3958.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
V. A. Cavicchio, J. K. Pru, B. S. Davis, J. S. Davis, B. R. Rueda, and D. H. Townson
Secretion of Monocyte Chemoattractant Protein-1 by Endothelial Cells of the Bovine Corpus Luteum: Regulation by Cytokines But Not Prostaglandin F2{alpha}
Endocrinology, September 1, 2002; 143(9): 3582 - 3589.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Okuda, R. Sakumoto, Y. Uenoyama, B. Berisha, A. Miyamoto, and D. Schams
Tumor Necrosis Factor {alpha} Receptors in Microvascular Endothelial Cells from Bovine Corpus Luteum
Biol Reprod, October 1, 1999; 61(4): 1017 - 1022.
[Abstract] [Full Text]

Home page
 Hum ReprodHome page
K.E. Ratcliffe, F.W. Anthony, M.C. Richardson, and R.W. Stones
Morphology and functional characteristics of human ovarian microvascular endothelium
Hum. Reprod., June 1, 1999; 14(6): 1549 - 1554.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1993