|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 106, Issue 3 909-918, Copyright © 1993 by Company of Biologists
JOURNAL ARTICLES |
R Chen, CA Perrone, LA Amos and RW Linck
University of Minnesota, Department of Cell Biology and Neuroanatomy, Minneapolis 55455.
Tektins are a class of proteins that form filamentous polymers in the walls of ciliary and flagellar microtubules, and they may also be present in centrioles, centrosomes and mitotic spindles. We report here the cloning and sequencing of a cDNA for ciliary tektin B1. Comparison of the predicted amino acid sequence of tektin B1 with the previously published sequence for tektin A1 reveals several features that better define this class of proteins. Like tektin A1, the central region of the tektin B1 polypeptide chain is predicted to form a coiled-coil rod, consisting of four major alpha-helical regions that are separated by non-helical linkers. Between the central rod domains of tektins A and B there is a 34%/20% amino acid sequence identity/similarity, including equivalent 50-residue segments containing 36 identities, and a high probability of long-range structural homology. The tektin polypeptide chains are divided into two major segments that have significant sequence homology to each other, both within a given tektin chain and between tektins A and B, indicative of gene duplication events. The tektins have a secondary structure and molecular design similar to, but a low primary sequence homology with, intermediate filament proteins. Unlike tektin A1, tektin B1 lacks any part of the C-terminal IFP consensus sequence.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J.-A. Park and D. J. Tschumperlin Chronic Intermittent Mechanical Stress Increases MUC5AC Protein Expression Am. J. Respir. Cell Mol. Biol., October 1, 2009; 41(4): 459 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-a. Yanagisawa and R. Kamiya A Tektin Homologue Is Decreased in Chlamydomonas Mutants Lacking an Axonemal Inner-Arm Dynein Mol. Biol. Cell, May 1, 2004; 15(5): 2105 - 2115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ikeda, J. A. Brown, T. Yagi, J. M. Norrander, M. Hirono, E. Eccleston, R. Kamiya, and R. W. Linck Rib72, a Conserved Protein Associated with the Ribbon Compartment of Flagellar A-microtubules and Potentially Involved in the Linkage between Outer Doublet Microtubules J. Biol. Chem., February 21, 2003; 278(9): 7725 - 7734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Iguchi, H. Tanaka, Y. Nakamura, M. Nozaki, T. Fujiwara, and Y. Nishimune Cloning and characterization of the human tektin-t gene Mol. Hum. Reprod., June 1, 2002; 8(6): 525 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Ostrowski, K. Blackburn, K. M. Radde, M. B. Moyer, D. M. Schlatzer, A. Moseley, and R. C. Boucher A Proteomic Analysis of Human Cilia: Identification of Novel Components Mol. Cell. Proteomics, June 1, 2002; 1(6): 451 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Wolkowicz, S. Naaby-Hansen, A. R. Gamble, P. P. Reddi, C. J. Flickinger, and J. C. Herr Tektin B1 Demonstrates Flagellar Localization in Human Sperm Biol Reprod, January 1, 2002; 66(1): 241 - 250. [Abstract] [Full Text]
|
|
|
|
|
|
J. M. Norrander, A. M. deCathelineau, J. A. Brown, M. E. Porter, and R. W. Linck The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas Mol. Biol. Cell, January 1, 2000; 11(1): 201 - 215. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Norrander, M. Larsson, S. Stahl, C. Hoog, and R. Linck Expression of Ciliary Tektins in Brain and Sensory Development J. Neurosci., November 1, 1998; 18(21): 8912 - 8918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hinchcliffe and R. Linck Two proteins isolated from sea urchin sperm flagella: structural components common to the stable microtubules of axonemes and centrioles J. Cell Sci., January 3, 1998; 111(5): 585 - 595. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Norrander, R. Linck, and R. Stephens Transcriptional control of tektin A mRNA correlates with cilia development and length determination during sea urchin embryogenesis Development, January 6, 1995; 121(6): 1615 - 1623. [Abstract] [PDF]
|
|
|
|
|
|
W Steffen, E. Fajer, and R. Linck Centrosomal components immunologically related to tektins from ciliary and flagellar microtubules J. Cell Sci., January 8, 1994; 107(8): 2095 - 2105. [Abstract] [PDF]
|
|
