spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Olmedilla, A.
Right arrow Articles by Risueno, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Olmedilla, A.
Right arrow Articles by Risueno, M. C.

Journal of Cell Science, Vol 106, Issue 4 1333-1346, Copyright © 1993 by Company of Biologists

JOURNAL ARTICLES

Ultrastructural rRNA localization in plant cell nucleoli. RNA/RNA in situ hybridization, autoradiography and cytochemistry

A Olmedilla, PS Testillano, O Vicente, M Delseny and MC Risueno
Centro de Investigaciones Biologicas, Madrid, Spain.

The distribution of ribosomal transcripts in the plant nucleolus has been studied by non-isotopic in situ hybridization in ultrathin Lowicryl K4M sections and by high-resolution autoradiography after labelling with tritiated uridine. In parallel, cytochemical techniques were applied to localize RNA on different plant nucleolar components of Allium cepa L. root meristematic cells and Capsicum annuum L. pollen grains. For RNA/RNA in situ hybridization, several biotinylated single-stranded ribosomal RNA probes were used for mapping different fragments of the 18 S and the 25 S rRNA gene transcribed regions. Ribosomal RNAs (from pre-rRNAs to mature 18 and 25 S RNAs) were found in the nucleolus, in the dense fibrillar (DFC) and granular components (GC). Hybridization signal was found at the periphery of some fibrillar centres (FCs) with probes recognizing both 18 and 25 S rRNA sequences. A quantitative study was performed to analyze the significance of this labelling. Incorporation of tritiated uridine into roots was carried out and, later, after a long time-exposure, autoradiography revealed the presence of newly synthesized RNA mainly in the DFC and at the periphery of the FCs. The presence of RNA in these areas was also confirmed by the cytochemical techniques used in this study. Taken together, these data favour the hypothesis that transcription can begin at the periphery of the FCs, although we cannot exclude the possibility that the DFC plays a role in this process.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
G Prestamo, P. Testillano, O Vicente, P Gonzalez-Melendi, M. Coronado, C Wilson, E Heberle-Bors, and M. Risueno
Ultrastructural distribution of a MAP kinase and transcripts in quiescent and cycling plant cells and pollen grains
J. Cell Sci., January 4, 1999; 112(7): 1065 - 1076.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
A Majewska-Sawka and M. Rodriguez-Garcia
rRNA distribution during microspore development in anthers of Beta vulgaris L. quantitative in situ hybridization analysis
J. Cell Sci., January 4, 1996; 109(4): 859 - 866.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
S Besse and F Puvion-Dutilleul
Intranuclear retention of ribosomal RNAs in response to herpes simplex virus type 1 infection
J. Cell Sci., January 1, 1996; 109(1): 119 - 129.
[Abstract] [PDF]


© The Company of Biologists Ltd 1993