|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 1 145-157, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
A Jahraus, B Storrie, G Griffiths and M Desjardins
European Molecular Biology Laboratory, Cell Biology Programme, Heidelberg, Germany.
We have investigated the interactions occurring between the prelysosomal compartment, PLC/late endosome, and terminal lysosomes using an approach that allowed us to internalize and deliver specific tracers to these compartments, and look for evidence of their meeting. After internalization of sucrose, the lysosomes (sucrosomes), but not the PLC/late endosomes, became significantly swollen and visible in the light microscope. If invertase is then added to the medium it reaches the lysosomes where it cleaves sucrose into fructose and glucose. These sugars, unlike sucrose, can be transported into the cytosol, resulting in the disappearance of the sucrosomes. We previously showed that phagocytosed latex beads are delivered specifically to, and reside in, the PLC/late endosome, a stage earlier than the lysosomes in the endocytic pathway (Rabinowitz et al. (1992) J. Cell Biol. 116, 95-112). In the present study, we demonstrate that invertase conjugated to the latex beads, and thus immobilized in late endosomes, has access to the sucrose present in the more distal lysosomes. Experiments using nocodazole indicate that this retrograde fusion event requires the presence of an intact microtubule network. The simplest interpretation of our results is that the two compartments fuse, allowing for a retrograde transport of sucrose from the lysosomes to the PLC/late endosomes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. Kaufmann and J. P. Krise Niemann-Pick C1 Functions in Regulating Lysosomal Amine Content J. Biol. Chem., September 5, 2008; 283(36): 24584 - 24593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Burlak, A. R. Whitney, D. J. Mead, T. Hackstadt, and F. R. DeLeo Maturation of Human Neutrophil Phagosomes Includes Incorporation of Molecular Chaperones and Endoplasmic Reticulum Quality Control Machinery Mol. Cell. Proteomics, April 1, 2006; 5(4): 620 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ono, S. O. Kim, and J. Han Susceptibility of Lysosomes to Rupture Is a Determinant for Plasma Membrane Disruption in Tumor Necrosis Factor Alpha-Induced Cell Death Mol. Cell. Biol., January 15, 2003; 23(2): 665 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Gotthardt, H. J. Warnatz, O. Henschel, F. Bruckert, M. Schleicher, and T. Soldati High-Resolution Dissection of Phagosome Maturation Reveals Distinct Membrane Trafficking Phases Mol. Biol. Cell, October 1, 2002; 13(10): 3508 - 3520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Clarke, J. Kohler, Q. Arana, T. Liu, J. Heuser, and G. Gerisch Dynamics of the vacuolar H+-ATPase in the contractile vacuole complex and the endosomal pathway of Dictyostelium cells J. Cell Sci., July 15, 2002; 115(14): 2893 - 2905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Astarie-Dequeker, S. Carreno, C. Cougoule, and I. Maridonneau-Parini The protein tyrosine kinase Hck is located on lysosomal vesicles that are physically and functionally distinct from CD63-positive lysosomes in human macrophages J. Cell Sci., January 1, 2002; 115(1): 81 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ono, X. Wang, and J. Han Resistance to Tumor Necrosis Factor-Induced Cell Death Mediated by PMCA4 Deficiency Mol. Cell. Biol., December 15, 2001; 21(24): 8276 - 8288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Plitz and K. Pfeffer Intact Lysosome Transport and Phagosome Function Despite Kinectin Deficiency Mol. Cell. Biol., September 1, 2001; 21(17): 6044 - 6055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. GOTO, K. MIZUNASHI, N. KIMURA, and Y. FURUKAWA Decreased Sensitivity of Distal Nephron and Collecting Duct to Parathyroid Hormone in Pseudohypoparathyroidism Type I J. Am. Soc. Nephrol., September 1, 2001; 12(9): 1965 - 1970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Al-Haddad, M. A. Shonn, B. Redlich, A. Blocker, J. K. Burkhardt, H. Yu, J. A. Hammer III, D. G. Weiss, W. Steffen, G. Griffiths, et al. Myosin Va Bound to Phagosomes Binds to F-Actin and Delays Microtubule-dependent Motility Mol. Biol. Cell, September 1, 2001; 12(9): 2742 - 2755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. L. Clemens, B.-Y. Lee, and M. A. Horwitz Mycobacterium tuberculosis and Legionella pneumophila Phagosomes Exhibit Arrested Maturation despite Acquisition of Rab7 Infect. Immun., September 1, 2000; 68(9): 5154 - 5166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hashim, K. Mukherjee, M. Raje, S. K. Basu, and A. Mukhopadhyay Live Salmonella Modulate Expression of Rab Proteins to Persist in a Specialized Compartment and Escape Transport to Lysosomes J. Biol. Chem., May 19, 2000; 275(21): 16281 - 16288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Clemens, B.-Y. Lee, and M. A. Horwitz Deviant Expression of Rab5 on Phagosomes Containing the Intracellular Pathogens Mycobacterium tuberculosis and Legionella pneumophila Is Associated with Altered Phagosomal Fate Infect. Immun., May 1, 2000; 68(5): 2671 - 2684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bucci, P. Thomsen, P. Nicoziani, J. McCarthy, and B. van Deurs Rab7: A Key to Lysosome Biogenesis Mol. Biol. Cell, February 1, 2000; 11(2): 467 - 480. [Abstract] [Full Text]
|
|
|
|
|
|
S Duclos, R Diez, J Garin, B Papadopoulou, A Descoteaux, H Stenmark, and M Desjardins Rab5 regulates the kiss and run fusion between phagosomes and endosomes and the acquisition of phagosome leishmanicidal properties in RAW 264.7 macrophages J. Cell Sci., January 10, 2000; 113(19): 3531 - 3541. [Abstract] [PDF]
|
|
|
|
|
|
M. GOTO and K. MIZUNASHI Calcitonin Stimulates Lysosomal Enzyme Release and Uptake in LLC-PK1 Cells J. Am. Soc. Nephrol., August 1, 1999; 10(8): 1640 - 1648. [Abstract] [Full Text]
|
|
|
|
|
|
J. A. Schmid, L. Mach, E. Paschke, and J. Glossl Accumulation of Sialic Acid in Endocytic Compartments Interferes with the Formation of Mature Lysosomes. IMPAIRED PROTEOLYTIC PROCESSING OF CATHEPSIN B IN FIBROBLASTS OF PATIENTS WITH LYSOSOMAL SIALIC ACID STORAGE DISEASE J. Biol. Chem., July 2, 1999; 274(27): 19063 - 19071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhen, S. Jiang, L. Feng, N. A. Bright, A. A. Peden, A. B. Seymour, E. K. Novak, R. Elliott, M. B. Gorin, M. S. Robinson, et al. Abnormal Expression and Subcellular Distribution of Subunit Proteins of the AP-3 Adaptor Complex Lead to Platelet Storage Pool Deficiency in the Pearl Mouse Blood, July 1, 1999; 94(1): 146 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Neufeld, M. Wastney, S. Patel, S. Suresh, A. M. Cooney, N. K. Dwyer, C. F. Roff, K. Ohno, J. A. Morris, E. D. Carstea, et al. The Niemann-Pick C1 Protein Resides in a Vesicular Compartment Linked to Retrograde Transport of Multiple Lysosomal Cargo J. Biol. Chem., April 2, 1999; 274(14): 9627 - 9635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Drmota, G. W. Gould, and G. Milligan Real Time Visualization of Agonist-mediated Redistribution and Internalization of a Green Fluorescent Protein-tagged Form of the Thyrotropin-releasing Hormone Receptor J. Biol. Chem., September 11, 1998; 273(37): 24000 - 24008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Bryant, R. C. Piper, L. S. Weisman, and T. H. Stevens Retrograde Traffic Out of the Yeast Vacuole to the TGN Occurs via the Prevacuolar/Endosomal Compartment J. Cell Biol., August 10, 1998; 142(3): 651 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Claus, A. Jahraus, T. Tjelle, T. Berg, H. Kirschke, H. Faulstich, and G. Griffiths Lysosomal Enzyme Trafficking between Phagosomes, Endosomes, and Lysosomes in J774 Macrophages. ENRICHMENT OF CATHEPSIN H IN EARLY ENDOSOMES J. Biol. Chem., April 17, 1998; 273(16): 9842 - 9851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhang, P. A. Hartz, E. Philip, L. C. Racusen, and P. W. Majerus Cell Lines from Kidney Proximal Tubules of a Patient with Lowe Syndrome Lack OCRL Inositol Polyphosphate 5-Phosphatase and Accumulate Phosphatidylinositol 4,5-Bisphosphate J. Biol. Chem., January 16, 1998; 273(3): 1574 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Blocker, G Griffiths, J. Olivo, A. Hyman, and F. Severin A role for microtubule dynamics in phagosome movement J. Cell Sci., January 2, 1998; 111(3): 303 - 312. [Abstract] [PDF]
|
|
|
|
|
|
A. Blocker, F. F. Severin, J. K. Burkhardt, J. B. Bingham, H. Yu, J.-C. Olivo, T. A. Schroer, A. A. Hyman, and G. Griffiths Molecular Requirements for Bi-directional Movement of Phagosomes Along Microtubules J. Cell Biol., April 7, 1997; 137(1): 113 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Gruenheid, E. Pinner, M. Desjardins, and P. Gros Natural Resistance to Infection with Intracellular Pathogens: The Nramp1 Protein Is Recruited to the Membrane of the Phagosome J. Exp. Med., February 17, 1997; 185(4): 717 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bright, B. Reaves, B. Mullock, and J. Luzio Dense core lysosomes can fuse with late endosomes and are re-formed from the resultant hybrid organelles J. Cell Sci., January 9, 1997; 110(17): 2027 - 2040. [Abstract] [PDF]
|
|
|
|
|
|
A. Bakker, P Webster, W. Jacob, and N. Andrews Homotypic fusion between aggregated lysosomes triggered by elevated [Ca2+]i in fibroblasts J. Cell Sci., January 9, 1997; 110(18): 2227 - 2238. [Abstract] [PDF]
|
|
|
|
|
|
M Desjardins, N. Nzala, R Corsini, and C Rondeau Maturation of phagosomes is accompanied by changes in their fusion properties and size-selective acquisition of solute materials from endosomes J. Cell Sci., January 9, 1997; 110(18): 2303 - 2314. [Abstract] [PDF]
|
|
|
|
|
|
H. Collins, U. Schaible, J. Ernst, and D. Russell Transfer of phagocytosed particles to the parasitophorous vacuole of Leishmania mexicana is a transient phenomenon preceding the acquisition of annexin I by the phagosome J. Cell Sci., January 1, 1997; 110(2): 191 - 200. [Abstract] [PDF]
|
|
|
|
|
|
A. Blocker, F. F. Severin, A. Habermann, A. A. Hyman, G. Griffiths, and J. K. Burkhardt Microtubule-associated Protein-dependent Binding of Phagosomes to Microtubules J. Biol. Chem., February 16, 1996; 271(7): 3803 - 3811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tjelle, A Brech, L. Juvet, G Griffiths, and T Berg Isolation and characterization of early endosomes, late endosomes and terminal lysosomes: their role in protein degradation J. Cell Sci., January 12, 1996; 109(12): 2905 - 2914. [Abstract] [PDF]
|
|
|
|
|
|
B. Reaves, N. Bright, B. Mullock, and J. Luzio The effect of wortmannin on the localisation of lysosomal type I integral membrane glycoproteins suggests a role for phosphoinositide 3-kinase activity in regulating membrane traffic late in the endocytic pathway J. Cell Sci., January 4, 1996; 109(4): 749 - 762. [Abstract] [PDF]
|
|
|
|
|
|
S Meresse, J. Gorvel, and P Chavrier The rab7 GTPase resides on a vesicular compartment connected to lysosomes J. Cell Sci., January 11, 1995; 108(11): 3349 - 3358. [Abstract] [PDF]
|
|
