|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 1 329-338, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
JC Brown, H Wiedemann and R Timpl
Max-Planck-Institut fur Biochemie, Martinsried, Germany.
Two isoforms of laminin were extracted from human placenta by neutral buffer containing EDTA, copurified through several steps and finally separated by Mono Q anion exchange chromatography. One variant consisted of disulphide-linked 340, 230 and 190 kDa subunits, which were identified by immunoblotting as Am, B1e and B2e chains. In the other variant, the B1e chain was replaced by B1s of 180 kDa. After rotary shadowing, both variants showed a similar cross-shaped structure. The nidogen content of these laminins was substoichiometric and variable (3-70%), indicating loss by endogenous proteolysis. Yet both human isoforms were able to bind mouse nidogen with an affinity (Kd approximately 0.5 nM) comparable to that of AeB1eB2e laminin from a mouse tumour. Since the binding site is known to be contributed by a single EGF-like motif of the B2e chain, this demonstrates that activity of this site is independent of chain assembly. Binding activity of both isoforms to collagen IV and the heparan sulphate proteoglycan perlecan was correlated to the nidogen content and could be enhanced by adding nidogen. Binding to heparin was only partial and heparin did not inhibit perlecan binding. This indicated a crucial role for nidogen in mediating the integration of these laminin isoforms into basement membranes. Variant AmB1sB2e showed calcium-dependent binding to fibulin-1, while only a little activity was found for AmB1eB2e. Both isoforms promoted adhesion and spreading of several cell lines. Adhesion could be completely inhibited by antibodies to the integrin beta 1 subunit but not, or only weakly, by antibodies against beta 3, alpha 2, alpha 3, alpha 5 and alpha 6 subunits. No inhibition was observed with an Arg-Gly-Asp-containing peptide.
This article has been cited by other articles:
|
|
 |
|
  K. Yuasa, S. Fukumoto, Y. Kamasaki, A. Yamada, E. Fukumoto, K. Kanaoka, K. Saito, H. Harada, E. Arikawa-Hirasawa, Y. Miyagoe-Suzuki, et al. Laminin {alpha}2 Is Essential for Odontoblast Differentiation Regulating Dentin Sialoprotein Expression J. Biol. Chem., March 12, 2004; 279(11): 10286 - 10292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. He, K. J. Jones, N. Vignier, G. Morgan, M. Chevallay, A. Barois, B. Estournet-Mathiaud, H. Hori, T. Mizuta, F. M.S. Tome, et al. Congenital muscular dystrophy with primary partial laminin {alpha}2 chain deficiency: Molecular study Neurology, October 9, 2001; 57(7): 1319 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Korhonen and I. Virtanen Immunohistochemical Localization of Laminin and Fibronectin Isoforms in Human Placental Villi J. Histochem. Cytochem., March 1, 2001; 49(3): 313 - 322. [Abstract] [Full Text]
|
|
|
|
 |
|
 U. Siler, M. Seiffert, S. Puch, A. Richards, B. Torok-Storb, C. A. Muller, L. Sorokin, and G. Klein Characterization and functional analysis of laminin isoforms in human bone marrow Blood, December 15, 2000; 96(13): 4194 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Miosge, F. Quondamatteo, C. Klenczar, and R. Herken Nidogen-1: Expression and Ultrastructural Localization During the Onset of Mesoderm Formation in the Early Mouse Embryo J. Histochem. Cytochem., February 1, 2000; 48(2): 229 - 238. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Burkin, J. Kim, M Gu, and S. Kaufman Laminin and alpha7beta1 integrin regulate agrin-induced clustering of acetylcholine receptors J. Cell Sci., January 8, 2000; 113(16): 2877 - 2886. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Colognato, D. A. Winkelmann, and P. D. Yurchenco Laminin Polymerization Induces a Receptor-Cytoskeleton Network J. Cell Biol., May 3, 1999; 145(3): 619 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. White, D. R. Dorscheid, K. F. Rabe, K. R. Wojcik, and K. J. Hamann Role of Very Late Adhesion Integrins in Mediating Repair of Human Airway Epithelial Cell Monolayers after Mechanical Injury Am. J. Respir. Cell Mol. Biol., April 1, 1999; 20(4): 787 - 796. [Abstract] [Full Text]
|
|
|
|
|
|
M Ferletta and P Ekblom Identification of laminin-10/11 as a strong cell adhesive complex for a normal and a malignant human epithelial cell line J. Cell Sci., January 1, 1999; 112(1): 1 - 10. [Abstract] [PDF]
|
|
|
|
|
|
E. L. McDearmon, A. L. Burwell, A. C. Combs, B. A. Renley, M. T. Sdano, and J. M. Ervasti Differential Heparin Sensitivity of alpha -Dystroglycan Binding to Laminins Expressed in Normal and dy/dy Mouse Skeletal Muscle J. Biol. Chem., September 11, 1998; 273(37): 24139 - 24144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kikkawa, N. Sanzen, and K. Sekiguchi Isolation and Characterization of Laminin-10/11 Secreted by Human Lung Carcinoma Cells. LAMININ-10/11 MEDIATES CELL ADHESION THROUGH INTEGRIN alpha 3beta 1 J. Biol. Chem., June 19, 1998; 273(25): 15854 - 15859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Dogic, P Rousselle, and M Aumailley Cell adhesion to laminin 1 or 5 induces isoform-specific clustering of integrins and other focal adhesion components J. Cell Sci., January 3, 1998; 111(6): 793 - 802. [Abstract] [PDF]
|
|
|
|
|
|
Y.-S. Cheng, M.-F. Champliaud, R. E. Burgeson, M. P. Marinkovich, and P. D. Yurchenco Self-assembly of Laminin Isoforms J. Biol. Chem., December 12, 1997; 272(50): 31525 - 31532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Colognato, M. MacCarrick, J. J. O'Rear, and P. D. Yurchenco The Laminin alpha 2-Chain Short Arm Mediates Cell Adhesion through Both the alpha 1beta 1 and alpha 2beta 1 Integrins J. Biol. Chem., November 14, 1997; 272(46): 29330 - 29336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tran, W. J. VanDusen, and W. S. Argraves The Self-association and Fibronectin-binding Sites of Fibulin-1 Map to Calcium-binding Epidermal Growth Factor-like Domains J. Biol. Chem., September 5, 1997; 272(36): 22600 - 22606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Reinhardt, T. Sasaki, B. J. Dzamba, D. R. Keene, M.-L. Chu, W. Gohring, R. Timpl, and L. Y. Sakai Fibrillin-1 and Fibulin-2 Interact and Are Colocalized in Some Tissues J. Biol. Chem., August 9, 1996; 271(32): 19489 - 19496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Pall, K. M. Bolton, and J. M. Ervasti Differential Heparin Inhibition of Skeletal Muscle alpha-Dystroglycan Binding to Laminins J. Biol. Chem., February 16, 1996; 271(7): 3817 - 3821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Miner, R. M. Lewis, and J. R. Sanes Molecular Cloning of a Novel Laminin Chain, alpha5, and Widespread Expression in Adult Mouse Tissues J. Biol. Chem., December 1, 1995; 270(48): 28523 - 28526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tran, A. Tanaka, S. V. Litvinovich, L. V. Medved, C. C. Haudenschild, and W. S. Argraves The Interaction of Fibulin-1 with Fibrinogen J. Biol. Chem., August 18, 1995; 270(33): 19458 - 19464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rousselle, R. Golbik, M. v. d. Rest, and M. Aumailley Structural Requirement for Cell Adhesion to Kalinin (Laminin-5) J. Biol. Chem., June 9, 1995; 270(23): 13766 - 13770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Schuler and L. Sorokin Expression of laminin isoforms in mouse myogenic cells in vitro and in vivo J. Cell Sci., January 12, 1995; 108(12): 3795 - 3805. [Abstract] [PDF]
|
|
|
|
|
|
R Brandenberger and M Chiquet Distinct heparin-binding and neurite-promoting properties of laminin isoforms isolated from chick heart J. Cell Sci., January 9, 1995; 108(9): 3099 - 3108. [Abstract] [PDF]
|
|
