|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 10 2729-2738, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
G Rimmington, B Dalby and DM Glover
Department of Anatomy & Physiology, University of Dundee, Scotland, UK.
We have introduced an N-terminally truncated form of cyclin B into the Drosophila germ-line downstream of the yeast upstream activator that responds to GAL4. When such lines of flies are crossed to lines in which GAL4 is expressed in imaginal discs and larval brain, the majority of the resulting progeny die at the late pupal stage of development. Very rarely (< 0.1% of progeny) adults emerge that have a mutant phenotype typical of flies with mutations in genes required for the cell cycle; they have rough eyes, deformed wings, abnormal bristles, and die within hours of emergence. The brains of third instar larval progeny show an abnormally high proportion of mitotic cells containing overcondensed chromatids that have undergone anaphase separation, together with cells that cannot be assigned to a particular mitotic stage. Immunostaining indicates that these anaphase cells contain moderate levels of cyclin B, suggesting that persistent p34cdc2 kinase activity can prevent progression from anaphase into telophase.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  R. M. Douglas, R. Farahani, P. Morcillo, A. Kanaan, T. Xu, and G. G. Haddad Hypoxia induces major effects on cell cycle kinetics and protein expression in Drosophila melanogaster embryos Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R511 - R521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Weingartner, M.-C. Criqui, T. Meszaros, P. Binarova, A.-C. Schmit, A. Helfer, A. Derevier, M. Erhardt, L. Bogre, and P. Genschik Expression of a Nondegradable Cyclin B1 Affects Plant Development and Leads to Endomitosis by Inhibiting the Formation of a Phragmoplast PLANT CELL, March 1, 2004; 16(3): 643 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Deak, M. Donaldson, and D. M. Glover Mutations in makos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A J. Cell Sci., October 15, 2003; 116(20): 4147 - 4158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Chang, N. Xu, and K. Q. Luo Degradation of Cyclin B Is Required for the Onset of Anaphase in Mammalian Cells J. Biol. Chem., September 26, 2003; 278(39): 37865 - 37873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Bentley, B. C. Williams, M. L. Goldberg, and A. J. Andres Phenotypic characterization of Drosophila ida mutants: defining the role of APC5 in cell cycle progression J. Cell Sci., January 3, 2002; 115(5): 949 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Donaldson, A. A.M. Tavares, H. Ohkura, P. Deak, and D. M. Glover Metaphase Arrest with Centromere Separation in polo Mutants of Drosophila J. Cell Biol., May 14, 2001; 153(4): 663 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Geley, E. Kramer, C. Gieffers, J. Gannon, J.-M. Peters, and T. Hunt Anaphase-Promoting Complex/Cyclosome-Dependent Proteolysis of Human Cyclin a Starts at the Beginning of Mitosis and Is Not Subject to the Spindle Assembly Checkpoint J. Cell Biol., April 2, 2001; 153(1): 137 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Leismann, A. Herzig, S. Heidmann, and C. F. Lehner Degradation of Drosophila PIM regulates sister chromatid separation during mitosis Genes & Dev., September 1, 2000; 14(17): 2192 - 2205. [Abstract] [Full Text]
|
|
|
|
|
|
P. Genschik, M. C. Criqui, Y. Parmentier, A. Derevier, and J. Fleck Cell Cycle –Dependent Proteolysis in Plants: Identification of the Destruction Box Pathway and Metaphase Arrest Produced by the Proteasome Inhibitor MG132 PLANT CELL, December 1, 1998; 10(12): 2063 - 2076. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. G. Kolonin and R. L. Finley Jr. Targeting cyclin-dependent kinases in Drosophila with peptide aptamers PNAS, November 24, 1998; 95(24): 14266 - 14271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Jaspersen, J. F. Charles, R. L. Tinker-Kulberg, and D. O. Morgan A Late Mitotic Regulatory Network Controlling Cyclin Destruction in Saccharomyces cerevisiae Mol. Biol. Cell, October 1, 1998; 9(10): 2803 - 2817. [Abstract] [Full Text]
|
|
|
|
|
|
P. Jin, S. Hardy, and D. O. Morgan Nuclear Localization of Cyclin B1 Controls Mitotic Entry After DNA Damage J. Cell Biol., May 18, 1998; 141(4): 875 - 885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B Bello, D Resendez-Perez, and W. Gehring Spatial and temporal targeting of gene expression in Drosophila by means of a tetracycline-dependent transactivator system Development, January 6, 1998; 125(12): 2193 - 2202. [Abstract] [PDF]
|
|
|
|
|
|
S. P. Wheatley, E. H. Hinchcliffe, M. Glotzer, A. A. Hyman, G. Sluder, and Y.-l. Wang CDK1 Inactivation Regulates Anaphase Spindle Dynamics and Cytokinesis In Vivo J. Cell Biol., July 28, 1997; 138(2): 385 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Page and T. Orr-Weaver The Drosophila genes grauzone and cortex are necessary for proper female meiosis J. Cell Sci., January 7, 1996; 109(7): 1707 - 1715. [Abstract] [PDF]
|
|
|
|
|
|
J. Paulson, J. Patzlaff, and A. Vallis Evidence that the endogenous histone H1 phosphatase in HeLa mitotic chromosomes is protein phosphatase 1, not protein phosphatase 2A J. Cell Sci., January 6, 1996; 109(6): 1437 - 1447. [Abstract] [PDF]
|
|
|
|
|
|
F Girard, A Fernandez, and N Lamb Delayed cyclin A and B1 degradation in non-transformed mammalian cells J. Cell Sci., January 7, 1995; 108(7): 2599 - 2608. [Abstract] [PDF]
|
|
