|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 10 2813-2824, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
KA Morano and DJ Klionsky
Section of Microbiology, University of California, Davis 95616.
Lysosomal/vacuolar protein targeting is dependent on compartment acidification. In yeast, sorting of soluble vacuolar proteins such as carboxypeptidase Y is sensitive to acute changes in vacuolar pH. In contrast, the vacuolar membrane protein alkaline phosphatase is missorted only under conditions of chronic deacidification. We have undertaken a temporal analysis to define further the relationship between compartment acidification and sorting of soluble and membrane vacuolar proteins. Depletion of either the Vma3p or Vma4p subunits of the yeast vacuolar ATPase over time resulted in loss of vacuolar ATPase activity and vacuolar acidification. A kinetic delay in processing of carboxypeptidase Y occurred concomitant with these physiological changes while transport of alkaline phosphatase remained unaffected. Carboxypeptidase S, another vacuolar hydrolase that transits through the secretory pathway as an integral membrane protein, displayed a pH sensitivity similar to that of soluble vacuolar proteins. These results indicate that compartment acidification is tightly coupled to efficient targeting of proteins to the vacuole and that there may be multiple distinct mechanisms for targeting of vacuolar membrane proteins.
This article has been cited by other articles:
|
|
 |
|
  V. Makrantoni, P. Dennison, M. J. R. Stark, and P. J. Coote A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance Microbiology, December 1, 2007; 153(12): 4016 - 4026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hayashi, T. Fukuzawa, H. Sorimachi, and T. Maeda Constitutive Activation of the pH-Responsive Rim101 Pathway in Yeast Mutants Defective in Late Steps of the MVB/ESCRT Pathway Mol. Cell. Biol., November 1, 2005; 25(21): 9478 - 9490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Lu, W. Qin, J. Li, N. Tan, D. Pan, H. Zhang, L. Xie, G. Yao, H. Shu, M. Yao, et al. The Growth and Metastasis of Human Hepatocellular Carcinoma Xenografts Are Inhibited by Small Interfering RNA Targeting to the Subunit ATP6L of Proton Pump Cancer Res., August 1, 2005; 65(15): 6843 - 6849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kawasaki-Nishi, K. Bowers, T. Nishi, M. Forgac, and T. H. Stevens The Amino-terminal Domain of the Vacuolar Proton-translocating ATPase a Subunit Controls Targeting and in Vivo Dissociation, and the Carboxyl-terminal Domain Affects Coupling of Proton Transport and ATP Hydrolysis J. Biol. Chem., December 7, 2001; 276(50): 47411 - 47420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bowers, B. P. Levi, F. I. Patel, and T. H. Stevens The Sodium/Proton Exchanger Nhx1p Is Required for Endosomal Protein Trafficking in the Yeast Saccharomyces cerevisiae Mol. Biol. Cell, December 1, 2000; 11(12): 4277 - 4294. [Abstract] [Full Text]
|
|
|
|
|
|
W.-P. Huang, S. V. Scott, J. Kim, and D. J. Klionsky The Itinerary of a Vesicle Component, Aut7p/Cvt5p, Terminates in the Yeast Vacuole via the Autophagy/Cvt Pathways J. Biol. Chem., February 25, 2000; 275(8): 5845 - 5851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Pearce, C. J. Carr, B. Das, and F. Sherman Phenotypic reversal of the btn1 defects in yeast by chloroquine: A yeast model for Batten disease PNAS, September 28, 1999; 96(20): 11341 - 11345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Sze, X. Li, and M. G. Palmgren Energization of Plant Cell Membranes by H+-Pumping ATPases: Regulation and Biosynthesis PLANT CELL, April 1, 1999; 11(4): 677 - 690. [Full Text]
|
|
|
|
 |
|
 J. D. Gary, A. E. Wurmser, C. J. Bonangelino, L. S. Weisman, and S. D. Emr Fab1p Is Essential for PtdIns(3)P 5-Kinase Activity and the Maintenance of Vacuolar Size and Membrane Homeostasis J. Cell Biol., October 5, 1998; 143(1): 65 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Zhang, K. J. Parra, J. Liu, and P. M. Kane Characterization of a Temperature-sensitive Yeast Vacuolar ATPase Mutant with Defects in Actin Distribution and Bud Morphology J. Biol. Chem., July 17, 1998; 273(29): 18470 - 18480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Tomashek, B. S. Garrison, and D. J. Klionsky Reconstitution in Vitro of the V1 Complex from the Yeast Vacuolar Proton-translocating ATPase. ASSEMBLY RECAPITULATES MECHANISM J. Biol. Chem., June 27, 1997; 272(26): 16618 - 16623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Tomashek, J. L. Sonnenburg, J. M. Artimovich, and D. J. Klionsky Resolution of Subunit Interactions and Cytoplasmic Subcomplexes of the Yeast Vacuolar Proton-translocating ATPase J. Biol. Chem., April 26, 1996; 271(17): 10397 - 10404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Perzov, H. Nelson, and N. Nelson Altered Distribution of the Yeast Plasma Membrane H+-ATPase as a Feature of Vacuolar H+-ATPase Null Mutants J. Biol. Chem., December 15, 2000; 275(51): 40088 - 40095. [Abstract] [Full Text] [PDF]
|
|
