|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 10 2825-2837, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
I Lajoie-Mazenc, Y Tollon, C Detraves, M Julian, A Moisand, C Gueth-Hallonet, A Debec, I Salles-Passador, A Puget, H Mazarguil and al. et
Laboratoire de Pharmacologie et de Toxicologie Fondamentales CNRS, Toulouse, France.
It has been claimed repeatedly that gamma-tubulin is exclusively localized at the spindle poles in mitotic animal cells, where it plays a role in microtubule nucleation. In addition to this localization, we have observed a gamma-tubulin-specific staining of the mitotic spindle in several animal cells (human, kangaroo rat, mouse, Chinese hamster, Xenopus and Drosophila) using five polyclonal antibodies raised against unique gamma-tubulin sequences and four different fixation protocols. In HeLa and PtK2 cells, gamma-tubulin was detected in the mitotic spindle from late prometaphase to telophase. In contrast, in other cell types, it was detected in metaphase only. In all cases we failed to detect gamma-tubulin in the short aster microtubules at the spindle poles. Electron microscopic observation revealed that at least part of the gamma-tubulin localized on the surface of spindle microtubules with a preferential distribution along kinetochore microtubules. In HeLa cells, the amount of antigenic gamma-tubulin was fairly constant in the spindle poles during mitosis from prometaphase to telophase. In contrast, gamma-tubulin appeared in the mitotic spindles in prometaphase. The amount of gamma-tubulin decreased in telophase, where it relocalized in the interzone. In metaphase cells about 15-25% of the total fluorescence was localized at the spindle poles, while 75-85% of the fluorescence was distributed over the rest of the spindle. These results suggest that the localization and timing of gamma-tubulin during the cell cycle is highly regulated and that is physiological role could be more complex and diverse than initially assumed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Bouissou, C. Verollet, A. Sousa, P. Sampaio, M. Wright, C. E. Sunkel, A. Merdes, and B. Raynaud-Messina {gamma}-Tubulin ring complexes regulate microtubule plus end dynamics J. Cell Biol., November 2, 2009; 187(3): 327 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Fant, N. Gnadt, L. Haren, and A. Merdes Stability of the small {gamma}-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus J. Cell Sci., April 15, 2009; 122(8): 1134 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Goshima, M. Mayer, N. Zhang, N. Stuurman, and R. D. Vale Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle J. Cell Biol., October 14, 2008; 181(3): 421 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liu and C. Wiese Xenopus NEDD1 is required for microtubule organization in Xenopus egg extracts J. Cell Sci., March 1, 2008; 121(5): 578 - 589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lee, H. M. Scherr, and J. B. Wallingford Shroom family proteins regulate {gamma}-tubulin distribution and microtubule architecture during epithelial cell shape change Development, April 1, 2007; 134(7): 1431 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wiese and Y. Zheng Microtubule nucleation: {gamma}-tubulin and beyond J. Cell Sci., October 15, 2006; 119(20): 4143 - 4153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Haren, M.-H. Remy, I. Bazin, I. Callebaut, M. Wright, and A. Merdes NEDD1-dependent recruitment of the {gamma}-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly J. Cell Biol., February 13, 2006; 172(4): 505 - 515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Verollet, N. Colombie, T. Daubon, H.-M. Bourbon, M. Wright, and B. Raynaud-Messina Drosophila melanogaster {gamma}-TuRC is dispensable for targeting {gamma}-tubulin to the centrosome and microtubule nucleation J. Cell Biol., February 13, 2006; 172(4): 517 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Janson, T. G. Setty, A. Paoletti, and P.T. Tran Efficient formation of bipolar microtubule bundles requires microtubule-bound {gamma}-tubulin complexes J. Cell Biol., April 25, 2005; 169(2): 297 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hertel and E. S. Mocarski Global Analysis of Host Cell Gene Expression Late during Cytomegalovirus Infection Reveals Extensive Dysregulation of Cell Cycle Gene Expression and Induction of Pseudomitosis Independent of US28 Function J. Virol., November 1, 2004; 78(21): 11988 - 12011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Raynaud-Messina, L. Mazzolini, A. Moisand, A.-M. Cirinesi, and M. Wright Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in {gamma}-tubulin-depleted cells J. Cell Sci., November 1, 2004; 117(23): 5497 - 5507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bergoglio, M.-J. Pillaire, M. Lacroix-Triki, B. Raynaud-Messina, Y. Canitrot, A. Bieth, M. Gares, M. Wright, G. Delsol, L. A. Loeb, et al. Deregulated DNA Polymerase {beta} Induces Chromosome Instability and Tumorigenesis Cancer Res., June 1, 2002; 62(12): 3511 - 3514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Li, J.-R. Ciu, Y. Ye, J.-M. Min, L.-H. Zhang, K. Wang, M. Gares, J. Cros, M. Wright, and J. Leung-Tack Antitumor activity of Z-ajoene, a natural compound purified from garlic: antimitotic and microtubule-interaction properties Carcinogenesis, April 1, 2002; 23(4): 573 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Buster, K. McNally, and F. J. McNally Katanin inhibition prevents the redistribution of {gamma}-tubulin at mitosis J. Cell Sci., January 3, 2002; 115(5): 1083 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Murphy, A. M. Preble, U. K. Patel, K. L. O'Connell, D. P. Dias, M. Moritz, D. Agard, J. T. Stults, and T. Stearns GCP5 and GCP6: Two New Members of the Human gamma -Tubulin Complex Mol. Biol. Cell, November 1, 2001; 12(11): 3340 - 3352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Jung, N. Prigozhina, C. E. Oakley, E. Nogales, and B. R. Oakley Alanine-scanning Mutagenesis of Aspergillus {gamma}-Tubulin Yields Diverse and Novel Phenotypes Mol. Biol. Cell, July 1, 2001; 12(7): 2119 - 2136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lee, T. Miyano, and R. M. Moor Spindle Formation and Dynamics of {gamma}-Tubulin and Nuclear Mitotic Apparatus Protein Distribution During Meiosis in Pig and Mouse Oocytes Biol Reprod, May 1, 2000; 62(5): 1184 - 1192. [Abstract] [Full Text]
|
|
|
|
|
|
V. Foe, C. Field, and G. Odell Microtubules and mitotic cycle phase modulate spatiotemporal distributions of F-actin and myosin II in Drosophila syncytial blastoderm embryos Development, January 5, 2000; 127(9): 1767 - 1787. [Abstract] [PDF]
|
|
|
|
|
|
F. Fava, B. Raynaud-Messina, J. Leung-Tack, L. Mazzolini, M. Li, J. C. Guillemot, D. Cachot, Y. Tollon, P. Ferrara, and M. Wright Human 76p: A New Member of the {gamma}-Tubulin-Associated Protein Family J. Cell Biol., November 15, 1999; 147(4): 857 - 868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Khodjakov and C. L. Rieder The Sudden Recruitment of {gamma}-Tubulin to the Centrosome at the Onset of Mitosis and Its Dynamic Exchange Throughout the Cell Cycle, Do Not Require Microtubules J. Cell Biol., August 9, 1999; 146(3): 585 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Megraw, K Li, L. Kao, and T. Kaufman The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila Development, January 7, 1999; 126(13): 2829 - 2839. [Abstract] [PDF]
|
|
|
|
|
|
S Llamazares, G Tavosanis, and C Gonzalez Cytological characterisation of the mutant phenotypes produced during early embryogenesis by null and loss-of-function alleles of the gammaTub37C gene in Drosophila J. Cell Sci., January 3, 1999; 112(5): 659 - 667. [Abstract] [PDF]
|
|
|
|
|
|
S. M. Murphy, L. Urbani, and T. Stearns The Mammalian {gamma}-Tubulin Complex Contains Homologues of the Yeast Spindle Pole Body Components Spc97p and Spc98p J. Cell Biol., May 4, 1998; 141(3): 663 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-M. Tassin, C. Celati, M. Moudjou, and M. Bornens Characterization of the Human Homologue of the Yeast Spc98p and Its Association with {gamma}-Tubulin J. Cell Biol., May 4, 1998; 141(3): 689 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U Euteneuer, R Graf, E Kube-Granderath, and M Schliwa Dictyostelium gamma-tubulin: molecular characterization and ultrastructural localization J. Cell Sci., January 2, 1998; 111(3): 405 - 412. [Abstract] [PDF]
|
|
|
|
|
|
R. Heald, R. Tournebize, A. Habermann, E. Karsenti, and A. Hyman Spindle Assembly in Xenopus Egg Extracts: Respective Roles of Centrosomes and Microtubule Self-Organization J. Cell Biol., August 11, 1997; 138(3): 615 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V Scott, T Sherwin, and K Gull gamma-tubulin in trypanosomes: molecular characterisation and localisation to multiple and diverse microtubule organising centres J. Cell Sci., January 1, 1997; 110(2): 157 - 168. [Abstract] [PDF]
|
|
|
|
|
|
I Lajoie-Mazenc, C Detraves, V Rotaru, M Gares, Y Tollon, C Jean, M Julian, M Wright, and B Raynaud-Messina A single gamma-tubulin gene and mRNA, but two gamma-tubulin polypeptides differing by their binding to the spindle pole organizing centres J. Cell Sci., January 10, 1996; 109(10): 2483 - 2492. [Abstract] [PDF]
|
|
|
|
|
|
M Moudjou, N Bordes, M Paintrand, and M Bornens gamma-Tubulin in mammalian cells: the centrosomal and the cytosolic forms J. Cell Sci., January 4, 1996; 109(4): 875 - 887. [Abstract] [PDF]
|
|
|
|
|
|
A Debec, C Detraves, C Montmory, G Geraud, and M Wright Polar organization of gamma-tubulin in acentriolar mitotic spindles of Drosophila melanogaster cells J. Cell Sci., January 7, 1995; 108(7): 2645 - 2653. [Abstract] [PDF]
|
|
|
|
|
|
R. Burns Analysis of the gamma-tubulin sequences: implications for the functional properties of gamma-tubulin J. Cell Sci., January 6, 1995; 108(6): 2123 - 2130. [PDF]
|
|
|
|
 |
|
 D. Chen, W. Xu, P. He, E. E. Medrano, and S. W. Whiteheart Gaf-1, a gamma -SNAP-binding Protein Associated with the Mitochondria J. Biol. Chem., April 13, 2001; 276(16): 13127 - 13135. [Abstract] [Full Text] [PDF]
|
|
