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Journal of Cell Science, Vol 107, Issue 10 2851-2859, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

TRiC-P5, a novel TCP1-related protein, is localized in the cytoplasm and in the nuclear matrix

EC Joly, E Tremblay, RM Tanguay, Y Wu and V Bibor-Hardy
Institut du Cancer de Montreal, Centre de Recherche Louis-Charles Simard, Quebec, Canada.

We have recently reported the cloning of a novel protein, TRiC-P5, with significant homology with protein 1 of the t-complex (TCP1). In the present study, the cellular localization of TRiC-P5 in Raji cells has been determined using an antiserum raised against a 18.5 kDa fusion protein. Results from cell fractionation and immunoblot studies indicate that TRiC-P5 is mainly localized in the cytoplasm. In addition, a significant part of TRiC-P5 is also found in the nucleus where it is attached to the nuclear matrix, a complex filament network involved in essential cellular functions such as DNA replication, and RNA transcription and maturation. Immunofluorescence experiments using the anti-TRiC-P5 antibodies confirm these results. We also provide evidence that, in the cytoplasm, TRiC-P5 is part of a large protein complex, most probably the TCP1-ring complex (TRiC), a hetero-oligomeric ring complex that plays a role of molecular chaperone in the folding of actin and tubulin.
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[Abstract] [Full Text]


© The Company of Biologists Ltd 1994