|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 12 3449-3459, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
CR Cowles, SD Emr and BF Horazdovsky
Division of Cellular and Molecular Medicine, University of California, San Diego, School of Medicine, La Jolla 92093-0668.
Genetic analyses of vacuolar protein sorting in Saccharomyces cerevisiae have uncovered a large number of mutants (vps) that missort and secrete vacuolar hydrolases. A small subset of vps mutants exhibit a temperature-conditional growth phenotype and show a severe defect in the localization of soluble vacuolar proteins, yet maintain a near-normal vacuole structure. Here, we report on the cloning and characterization of the gene affected in one of these mutants, VPS45, which has been found to encode a member of a protein family that includes the yeast proteins Sec1p, Sly1p and Vps33p, as well as n-Sec1, UNC18 and Rop from other eukaryotic organisms. These proteins are thought to participate in vesicle-mediated protein transport events. Polyclonal antiserum raised against a TrpE-Vps45 fusion protein specifically detects a stable 67 kDa protein in labeled yeast cell extracts. Subcellular fractionation studies demonstrate that the majority of Vps45p is associated with a high-speed membrane pellet fraction that includes Golgi, transport vesicles and, potentially, endosomal membranes. Significantly, this fraction lacks ER, vacuole and plasma membranes. Overexpression of Vps45p saturates the sites with which Vps45p associates. A vps45 null mutant accumulates vesicles, many of which were found to be present in large clusters. This accumulation of potential transport vesicles indicates that Vps45p may facilitate the targeting and/or fusion of these vesicles in the vacuolar protein sorting pathway.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Ma, M. Takeuchi, R. Sugiura, S. O. Sio, and T. Kuno Deletion mutants of AP-1 adaptin subunits display distinct phenotypes in fission yeast Genes Cells, August 1, 2009; 14(8): 1015 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Struthers, S. G. Shanks, C. MacDonald, L. N. Carpp, A. M. Drozdowska, D. Kioumourtzoglou, M. L. M. Furgason, M. Munson, and N. J. Bryant Functional homology of mammalian syntaxin 16 and yeast Tlg2p reveals a conserved regulatory mechanism J. Cell Sci., July 1, 2009; 122(13): 2292 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Akbar, S. Ray, and H. Kramer The SM Protein Car/Vps33A Regulates SNARE-mediated Trafficking to Lysosomes and Lysosome-related Organelles Mol. Biol. Cell, March 15, 2009; 20(6): 1705 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. L. Rodkey, S. Liu, M. Barry, and J. A. McNew Munc18a Scaffolds SNARE Assembly to Promote Membrane Fusion Mol. Biol. Cell, December 1, 2008; 19(12): 5422 - 5434. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Morrison, H. Dionne, T. E. Rusten, A. Brech, W. W. Fisher, B. D. Pfeiffer, S. E. Celniker, H. Stenmark, and D. Bilder Regulation of Early Endosomal Entry by the Drosophila Tumor Suppressors Rabenosyn and Vps45 Mol. Biol. Cell, October 1, 2008; 19(10): 4167 - 4176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Peng, C. Togawa, K. Zhang, and S. K. Kurdistani Regulators of Cellular Levels of Histone Acetylation in Saccharomyces cerevisiae Genetics, May 1, 2008; 179(1): 277 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyatake, T. Kuno, A. Kita, K. Katsura, K. Takegawa, S. Uno, T. Nabata, and R. Sugiura Valproic Acid Affects Membrane Trafficking and Cell-Wall Integrity in Fission Yeast Genetics, April 1, 2007; 175(4): 1695 - 1705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Knop, K. J. Miller, M. Mazza, D. Feng, M. Weber, S. Keranen, and J. Jantti Molecular Interactions Position Mso1p, a Novel PTB Domain Homologue, in the Interface of the Exocyst Complex and the Exocytic SNARE Machinery in Yeast Mol. Biol. Cell, October 1, 2005; 16(10): 4543 - 4556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Scott, J. S. Van Komen, H. Irshad, S. Liu, K. A. Wilson, and J. A. McNew Sec1p directly stimulates SNARE-mediated membrane fusion in vitro J. Cell Biol., October 11, 2004; 167(1): 75 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Gladycheva, C. S. Ho, Y. Y. F. Lee, and E. L. Stuenkel Regulation of syntaxin1A-munc18 complex for SNARE pairing in HEK293 cells J. Physiol., August 1, 2004; 558(3): 857 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Subramanian, C. A. Woolford, and E. W. Jones The Sec1/Munc18 Protein, Vps33p, Functions at the Endosome and the Vacuole of Saccharomyces cerevisiae Mol. Biol. Cell, June 1, 2004; 15(6): 2593 - 2605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pizzirusso and A. Chang Ubiquitin-mediated Targeting of a Mutant Plasma Membrane ATPase, Pma1-7, to the Endosomal/Vacuolar System in Yeast Mol. Biol. Cell, May 1, 2004; 15(5): 2401 - 2409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Bryant and D. E. James The Sec1p/Munc18 (SM) protein, Vps45p, cycles on and off membranes during vesicle transport J. Cell Biol., May 26, 2003; 161(4): 691 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Munir and V. Kapur Regulation of Host Cell Transcriptional Physiology by the Avian Pneumovirus Provides Key Insights into Host-Pathogen Interactions J. Virol., April 15, 2003; 77(8): 4899 - 4910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. D. Burgoyne and A. Morgan Secretory Granule Exocytosis Physiol Rev, April 1, 2003; 83(2): 581 - 632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Brickner, J. M. Blanchette, G. Sipos, and R. S. Fuller The Tlg SNARE complex is required for TGN homotypic fusion J. Cell Biol., December 10, 2001; 155(6): 969 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Helliwell, S. Losko, and C. A. Kaiser Components of a Ubiquitin Ligase Complex Specify Polyubiquitination and Intracellular Trafficking of the General Amino Acid Permease J. Cell Biol., May 14, 2001; 153(4): 649 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Abeliovich, W. A. Dunn Jr., J. Kim, and D. J. Klionsky Dissection of Autophagosome Biogenesis into Distinct Nucleation and Expansion Steps J. Cell Biol., November 27, 2000; 151(5): 1025 - 1034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Amerik, J. Nowak, S. Swaminathan, and M. Hochstrasser The Doa4 Deubiquitinating Enzyme Is Functionally Linked to the Vacuolar Protein-sorting and Endocytic Pathways Mol. Biol. Cell, October 1, 2000; 11(10): 3365 - 3380. [Abstract] [Full Text]
|
|
|
|
|
|
A. Srivastava, C. A. Woolford, and E. W. Jones Pep3p/Pep5p Complex: A Putative Docking Factor at Multiple Steps of Vesicular Transport to the Vacuole of Saccharomyces cerevisiae Genetics, September 1, 2000; 156(1): 105 - 122. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Bassham, A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel AtVPS45 Complex Formation at the trans-Golgi Network Mol. Biol. Cell, July 1, 2000; 11(7): 2251 - 2265. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Conboy and M. S. Cyert Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology. Mol. Biol. Cell, July 1, 2000; 11(7): 2429 - 2443. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Gerrard, N. J. Bryant, and T. H. Stevens VPS21 Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment Mol. Biol. Cell, February 1, 2000; 11(2): 613 - 626. [Abstract] [Full Text]
|
|
|
|
|
|
B. Yang, M. Steegmaier, L. C. Gonzalez Jr., and R. H. Scheller Nsec1 Binds a Closed Conformation of Syntaxin1a J. Cell Biol., January 24, 2000; 148(2): 247 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Beck, A. Schmidt, and M. N. Hall Starvation Induces Vacuolar Targeting and Degradation of the Tryptophan Permease in Yeast J. Cell Biol., September 20, 1999; 146(6): 1227 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. S. Coe, A. C. B. Lim, J. Xu, and W. Hong A Role for Tlg1p in the Transport of Proteins within the Golgi Apparatus of Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 1999; 10(7): 2407 - 2423. [Abstract] [Full Text]
|
|
|
|
|
|
G. G. Tall, H. Hama, D. B. DeWald, and B. F. Horazdovsky The Phosphatidylinositol 3-Phosphate Binding Protein Vac1p Interacts with a Rab GTPase and a Sec1p Homologue to Facilitate Vesicle-mediated Vacuolar Protein Sorting Mol. Biol. Cell, June 1, 1999; 10(6): 1873 - 1889. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Hama, G. G. Tall, and B. F. Horazdovsky Vps9p Is a Guanine Nucleotide Exchange Factor Involved in Vesicle-mediated Vacuolar Protein Transport J. Biol. Chem., May 21, 1999; 274(21): 15284 - 15291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Wurmser, J. D. Gary, and S. D. Emr Phosphoinositide 3-Kinases and Their FYVE Domain-containing Effectors as Regulators of Vacuolar/Lysosomal Membrane Trafficking Pathways J. Biol. Chem., April 2, 1999; 274(14): 9129 - 9132. [Full Text] [PDF]
|
|
|
|
|
|
A. I. Fletcher, R. Shuang, D. R. Giovannucci, L. Zhang, M. A. Bittner, and E. L. Stuenkel Regulation of Exocytosis by Cyclin-dependent Kinase 5 via Phosphorylation of Munc18 J. Biol. Chem., February 12, 1999; 274(7): 4027 - 4035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Gary, A. E. Wurmser, C. J. Bonangelino, L. S. Weisman, and S. D. Emr Fab1p Is Essential for PtdIns(3)P 5-Kinase Activity and the Maintenance of Vacuolar Size and Membrane Homeostasis J. Cell Biol., October 5, 1998; 143(1): 65 - 79. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Séron, V. Tieaho, C. Prescianotto-Baschong, T. Aust, M.-O. Blondel, P. Guillaud, G. Devilliers, O. W. Rossanese, B. S. Glick, H. Riezman, et al. A Yeast t-SNARE Involved in Endocytosis Mol. Biol. Cell, October 1, 1998; 9(10): 2873 - 2889. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. K. Sato, T. Darsow, and S. D. Emr Vam7p, a SNAP-25-Like Molecule, and Vam3p, a Syntaxin Homolog, Function Together in Yeast Vacuolar Protein Trafficking Mol. Cell. Biol., September 1, 1998; 18(9): 5308 - 5319. [Abstract] [Full Text]
|
|
|
|
|
|
T. Darsow, C. G. Burd, and S. D. Emr Acidic Di-leucine Motif Essential for AP-3-dependent Sorting and Restriction of the Functional Specificity of the Vam3p Vacuolar t-SNARE J. Cell Biol., August 24, 1998; 142(4): 913 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Bryant, R. C. Piper, L. S. Weisman, and T. H. Stevens Retrograde Traffic Out of the Yeast Vacuole to the TGN Occurs via the Prevacuolar/Endosomal Compartment J. Cell Biol., August 10, 1998; 142(3): 651 - 663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tamori, M. Kawanishi, T. Niki, H. Shinoda, S. Araki, H. Okazawa, and M. Kasuga Inhibition of Insulin-induced GLUT4 Translocation by Munc18c through Interaction with Syntaxin4 in 3T3-L1 Adipocytes J. Biol. Chem., July 31, 1998; 273(31): 19740 - 19746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Gerhardt, T. J. Kordas, C. M. Thompson, P. Patel, and T. Vida The Vesicle Transport Protein Vps33p Is an ATP-binding Protein That Localizes to the Cytosol in an Energy-dependent Manner J. Biol. Chem., June 19, 1998; 273(25): 15818 - 15829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Bassham and N. V. Raikhel An Arabidopsis VPS45p Homolog Implicated in Protein Transport to the Vacuole Plant Physiology, June 1, 1998; 117(2): 407 - 415. [Abstract] [Full Text]
|
|
|
|
|
|
M. Paidhungat and S. Garrett Cdc1 and the Vacuole Coordinately Regulate Mn2+ Homeostasis in the Yeast Saccharomyces cerevisiae Genetics, April 1, 1998; 148(4): 1787 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Bryant and T. H. Stevens Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole Microbiol. Mol. Biol. Rev., March 1, 1998; 62(1): 230 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shuang, L. Zhang, A. Fletcher, G. E. Groblewski, J. Pevsner, and E. L. Stuenkel Regulation of Munc-18/Syntaxin 1A Interaction by Cyclin-dependent Kinase 5 in Nerve Endings J. Biol. Chem., February 27, 1998; 273(9): 4957 - 4966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Riento, T Galli, S Jansson, C Ehnholm, E Lehtonen, and V. Olkkonen Interaction of Munc-18-2 with syntaxin 3 controls the association of apical SNAREs in epithelial cells J. Cell Sci., January 9, 1998; 111(17): 2681 - 2688. [Abstract] [PDF]
|
|
|
|
|
|
Pth1/Vam3p Is the Syntaxin Homolog at the Vacuolar Membrane of Saccharomyces cerevisae Required for the Delivery of Vacuolar Hydrolases Genetics, January 1, 1998; 148(1): 85 - 100.
|
|
|
|
|
|
J. D. Stepp, K. Huang, and S. K. Lemmon The Yeast Adaptor Protein Complex, AP-3, Is Essential for the Efficient Delivery of Alkaline Phosphatase by the Alternate Pathway to the Vacuole J. Cell Biol., December 29, 1997; 139(7): 1761 - 1774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Piper, N. J. Bryant, and T. H. Stevens The Membrane Protein Alkaline Phosphatase Is Delivered to the Vacuole by a Route That Is Distinct from the VPS-dependent Pathway J. Cell Biol., August 11, 1997; 138(3): 531 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Darsow, S. E. Rieder, and S. D. Emr A Multispecificity Syntaxin Homologue, Vam3p, Essential for Autophagic and Biosynthetic Protein Transport to the Vacuole J. Cell Biol., August 11, 1997; 138(3): 517 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Matsuo, S. Ogawa, T. Takagi, A. Wanaka, T. Mori, T. Matsuyama, D. J. Pinsky, D. M. Stern, and M. Tohyama Cloning of a Putative Vesicle Transport-related Protein, RA410, from Cultured Rat Astrocytes and Its Expression in Ischemic Rat Brain J. Biol. Chem., June 27, 1997; 272(26): 16438 - 16444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Radisky, W. B. Snyder, S. D. Emr, and J. Kaplan Characterization of VPS41, a gene required for vacuolar trafficking and high-affinity iron transport in yeast PNAS, May 27, 1997; 94(11): 5662 - 5666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Tellam, D. E. James, T. H. Stevens, and R. C. Piper Identification of a Mammalian Golgi Sec1p-like Protein, mVps45 J. Biol. Chem., March 7, 1997; 272(10): 6187 - 6193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. F. Horazdovsky, C. R. Cowles, P. Mustol, M. Holmes, and S. D. Emr A Novel RING Finger Protein, Vps8p, Functionally Interacts with the Small GTPase, Vps21p, to Facilitate Soluble Vacuolar Protein Localization J. Biol. Chem., December 27, 1996; 271(52): 33607 - 33615. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.H. Stack, P.K. Herman, D.B. DeWald, E.G. Marcusson, J. Lin Cereghino, B.F. Horazdovsky, and S.D. Emr Novel Protein Kinase/Phosphatidylinositol 3-Kinase Complex Essential for Receptor-mediated Protein Sorting to the Vacuole in Yeast Cold Spring Harb Symp Quant Biol, January 1, 1995; 60(0): 157 - 170. [Abstract] [PDF]
|
|
|
|
|
|
E. Harsay and R. Schekman A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway J. Cell Biol., January 21, 2002; 156(2): 271 - 286. [Abstract] [Full Text] [PDF]
|
|
