|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 12 3535-3543, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
MB Heintzelman, T Hasson and MS Mooseker
Department of Biology, Yale University, New Haven, CT 06511.
Representatives of class V and class VI unconventional myosins are identified as components of the intestinal brush border cytoskeleton. With brush border myosin-I and myosin-II, this brings to four the number of myosin classes associated with this one subcellular domain and represents the first characterization of four classes of myosins expressed in a single metazoan cell type. The distribution and cytoskeletal association of each myosin is distinct as assessed by both biochemical fractionation and immunofluorescence localization. Myosin-VI exists in both the microvillus and terminal web although the terminal web is the predominant site of concentration. Myosin-V is present in the terminal web and, most notably, at the distal ends of the microvilli, thus becoming the first actin-binding protein to be localized to this domain as assessed by both immunohistochemical and biochemical methods. In the undifferentiated enterocytes of the intestinal crypts, myosin-VI is expressed but not yet localized to the brush border, in contrast to myosin-V, which does demonstrate an apical distribution in these cells. An assessment of myosin abundance indicates that while myosin-II is the most abundant in the cell and in the brush border, brush border myosin-I is only slightly less abundant in contrast to myosins-V and -VI, both of which are two orders of magnitude less abundant than the others. Extraction studies indicate that of these four myosins, myosin-V is the most tightly associated with the brush border membrane, as detergent, in addition to ATP, is required for efficient solubilization.
This article has been cited by other articles:
|
|
 |
|
  R. E. McConnell and M. J. Tyska Myosin-1a powers the sliding of apical membrane along microvillar actin bundles J. Cell Biol., May 21, 2007; 177(4): 671 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Secor Evolutionary and Cellular Mechanisms Regulating Intestinal Performance of Amphibians and Reptiles Integr. Comp. Biol., April 1, 2005; 45(2): 282 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Waharte, C. M. Brown, S. Coscoy, E. Coudrier, and F. Amblard A Two-Photon FRAP Analysis of the Cytoskeleton Dynamics in the Microvilli of Intestinal Cells Biophys. J., February 1, 2005; 88(2): 1467 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Osterweil, D. G. Wells, and M. S. Mooseker A role for myosin VI in postsynaptic structure and glutamate receptor endocytosis J. Cell Biol., January 17, 2005; 168(2): 329 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Sato, H. D. White, A. Inoue, B. Belknap, R. Ikebe, and M. Ikebe Human Deafness Mutation of Myosin VI (C442Y) Accelerates the ADP Dissociation Rate J. Biol. Chem., July 9, 2004; 279(28): 28844 - 28854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hasson Myosin VI: two distinct roles in endocytosis J. Cell Sci., September 1, 2003; 116(17): 3453 - 3461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. L. TUMA and A. L. HUBBARD Transcytosis: Crossing Cellular Barriers Physiol Rev, July 1, 2003; 83(3): 871 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Aschenbrenner, T. Lee, and T. Hasson Myo6 Facilitates the Translocation of Endocytic Vesicles from Cell Peripheries Mol. Biol. Cell, July 1, 2003; 14(7): 2728 - 2743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Cramer Myosin VI: Roles for a Minus End-directed Actin Motor in Cells J. Cell Biol., September 18, 2000; 150(6): F121 - F126. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V Tsakraklides, K Krogh, L Wang, J. Bizario, R. Larson, E. Espreafico, and J. Wolenski Subcellular localization of GFP-myosin-V in live mouse melanocytes J. Cell Sci., January 9, 1999; 112(17): 2853 - 2865. [Abstract] [PDF]
|
|
|
|
|
|
F. Buss, J. Kendrick-Jones, C. Lionne, A. E. Knight, G. P. Cote, and J. Paul Luzio The Localization of Myosin VI at the Golgi Complex and Leading Edge of Fibroblasts and Its Phosphorylation and Recruitment into Membrane Ruffles of A431 Cells after Growth Factor Stimulation J. Cell Biol., December 14, 1998; 143(6): 1535 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hasson, P. G. Gillespie, J. A. Garcia, R. B. MacDonald, Y.-d. Zhao, A. G. Yee, M. S. Mooseker, and D. P. Corey Unconventional Myosins in Inner-Ear Sensory Epithelia J. Cell Biol., June 16, 1997; 137(6): 1287 - 1307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hasson and M. S. Mooseker Vertebrate Unconventional Myosins J. Biol. Chem., July 12, 1996; 271(28): 16431 - 16434. [Full Text] [PDF]
|
|
|
|
 |
|
 P.G. Gillespie, T. Hasson, J.A. Garcia, and D.P. Corey Multiple Myosin Isozymes and Hair-cell Function Cold Spring Harb Symp Quant Biol, January 1, 1996; 61(0): 309 - 318. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Biemesderfer, S. A. Mentone, M. Mooseker, and T. Hasson Expression of myosin VI within the early endocytic pathway in adult and developing proximal tubules Am J Physiol Renal Physiol, May 1, 2002; 282(5): F785 - F794. [Abstract] [Full Text] [PDF]
|
|
