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Journal of Cell Science, Vol 107, Issue 12 3655-3663, Copyright © 1994 by Company of Biologists


JOURNAL ARTICLES

Assembly of the cadherin-catenin complex in vitro with recombinant proteins

H Aberle, S Butz, J Stappert, H Weissig, R Kemler and H Hoschuetzky
Max-Planck-Institut fur Immunbiologie, Freiburg, Germany.

The cytoplasmic domain of classical cadherins is tightly associated with three proteins termed alpha-, beta- and gamma-catenin. These accessory proteins are of central importance for the adhesive properties of this class of cell adhesion molecules. In order to examine the molecular architecture of the cadherin-catenin complex in more detail we have expressed the catenins and the cytoplasmic domain of E-cadherin as fusion proteins in Escherichia coli, and analyzed the interaction of purified recombinant cadherin and catenins in combinatorial protein-protein interaction experiments. The cytoplasmic domain of E-cadherin cannot directly associate with alpha-catenin but interacts with high affinity with beta-catenin, whereas the binding of gamma-catenin (plakoglobin) to E-cadherin is less efficient. alpha- and beta-catenin assemble into a 1:1 heterodimeric complex. The analysis of various truncated beta-catenins revealed that an alpha-catenin binding site in beta-catenin is localized between amino acid positions 120 and 151. The central role of beta-catenin for the assembly of the heterotrimeric E-cadherin/alpha-catenin/beta-catenin complex in mixing experiments with all components was demonstrated. The reconstitution in vitro of the cadherin-catenin complex should allow the study of the interaction with signalling molecules and with the actin-based cytoskeleton.


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[Abstract] [Full Text] [PDF]


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[Abstract] [Full Text] [PDF]


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J. Cell Biol., August 7, 2000; 150(3): 567 - 580.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
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J. Cell Sci., June 8, 2000; 111(14): 1941 - 1949.
[Abstract] [PDF]


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Home page
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[Abstract] [Full Text] [PDF]


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Home page
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Home page
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Home page
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Home page
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[Abstract] [PDF]


Home page
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Mol. Cell. Biol.Home page
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