spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cunningham, A. C.
Right arrow Articles by Kirby, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cunningham, A. C.
Right arrow Articles by Kirby, J. A.

Journal of Cell Science, Vol 107, Issue 2 443-449, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

Constitutive expression of MHC and adhesion molecules by alveolar epithelial cells (type II pneumocytes) isolated from human lung and comparison with immunocytochemical findings

AC Cunningham, DS Milne, J Wilkes, JH Dark, TD Tetley and JA Kirby
Department of Surgery, University of Newcastle upon Tyne, UK.

Highly purified populations of alveolar epithelial cells (type II pneumocytes) were isolated from human lung specimens. These cells were characterised histochemically, by demonstrating the presence of intracellular alkaline phosphatase, and morphologically, by electron microscopic demonstration of lamellar bodies and microvilli. Expression of the epithelial glycoprotein HEA-125, of MHC class I and class II (HLA-DR, -DP and -DQ) antigens and of the intercellular adhesion molecules ICAM-1, VCAM-1, LFA-3 and B7 was quantified by flow cytometry. Comparison was made between the expression of these molecules by isolated type II cells and by alveolar epithelium in normal human lung tissue after immunocytochemical staining of frozen sections of donor lung. Isolated type II pneumocytes expressed HEA-125 and class I MHC molecules and the class II MHC molecules HLA-DR and -DP; HLA-DQ was not detected. The intercellular adhesion molecule ICAM-1 was expressed constitutively at low levels but there was minimal expression of VCAM-1, LFA-3 and B7. It was not possible to differentiate type II cells from the predominant type I pneumocytes on frozen sections. Alveolar epithelium expressed HEA-125, class I MHC antigens, the class II molecules HLA-DR, and -DP and the intercellular adhesion molecule LFA-3. Expression of the adhesion molecules ICAM-1, VCAM-1 and B7 was variable. As with the isolates, HLA-DQ was not observed on alveolar epithelium. In conclusion, a reproducible method for the isolation of pure populations of human type II pneumocytes has been developed. These cells were not damaged by the isolation procedure. It is not known whether alveolar epithelium can present antigens to T lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Vet PatholHome page
A. J. Buendia, J. Sanchez, C. M. Martinez, and J. A. Navarro
Immunohistochemical Characterization of a Pulmonary Large-Cell Carcinoma in a Dog
Vet. Pathol., July 1, 2008; 45(4): 484 - 488.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Lo, S. Hansen, K. Evans, J. K. Heath, and J. R. Wright
Alveolar Epithelial Type II Cells Induce T Cell Tolerance to Specific Antigen
J. Immunol., January 15, 2008; 180(2): 881 - 888.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Q. Tong, L. Zheng, L. Lin, B. Li, D. Wang, and D. Li
Hypoxia-Induced Mitogenic Factor Promotes Vascular Adhesion Molecule-1 Expression via the PI-3K/Akt-NF-{kappa}B Signaling Pathway
Am. J. Respir. Cell Mol. Biol., October 1, 2006; 35(4): 444 - 456.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. N. McNeilly, J. K. Brown, and G. Harkiss
Differential Expression of Cell Surface Markers by Ovine Respiratory Tract Dendritic Cells
J. Histochem. Cytochem., September 1, 2006; 54(9): 1021 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Fang, Y. Song, J. Hirsch, L. J. V. Galietta, N. Pedemonte, R. L. Zemans, G. Dolganov, A. S. Verkman, and M. A. Matthay
Contribution of CFTR to apical-basolateral fluid transport in cultured human alveolar epithelial type II cells
Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L242 - L249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
H. Debbabi, S. Ghosh, A. B. Kamath, J. Alt, D. E. deMello, S. Dunsmore, and S. M. Behar
Primary type II alveolar epithelial cells present microbial antigens to antigen-specific CD4+ T cells
Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L274 - L279.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. Ishizaka, T. Matsuda, K. H. Albertine, H. Koh, S. Tasaka, N. Hasegawa, N. Kohno, T. Kotani, H. Morisaki, J. Takeda, et al.
Elevation of KL-6, a lung epithelial cell marker, in plasma and epithelial lining fluid in acute respiratory distress syndrome
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1088 - L1094.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
B. Salaun, B. de Saint-Vis, N. Pacheco, Y. Pacheco, A. Riesler, S. Isaac, C. Leroux, V. Clair-Moninot, J.-J. Pin, J. Griffith, et al.
CD208/Dendritic Cell-Lysosomal Associated Membrane Protein Is a Marker of Normal and Transformed Type II Pneumocytes
Am. J. Pathol., March 1, 2004; 164(3): 861 - 871.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
B. Beck-Schimmer, C. Madjdpour, S. Kneller, U. Ziegler, T. Pasch, R.P. Wuthrich, P.A. Ward, and R.C. Schimmer
Role of alveolar epithelial ICAM-1 in lipopolysaccharide-induced lung inflammation
Eur. Respir. J., June 1, 2002; 19(6): 1142 - 1150.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. Beck-Schimmer, R. C. Schimmer, C. Madjdpour, J. M. Bonvini, T. Pasch, and P. A. Ward
Hypoxia Mediates Increased Neutrophil and Macrophage Adhesiveness to Alveolar Epithelial Cells
Am. J. Respir. Cell Mol. Biol., December 1, 2001; 25(6): 780 - 787.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Freek van Iwaarden, E. Claassen, S. H. M. Jeurissen, H. P. Haagsman, and G. Kraal
Alveolar Macrophages, Surfactant Lipids, and Surfactant Protein B Regulate the Induction of Immune Responses via the Airways
Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 452 - 458.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
C. Lawson, A. M. McCormack, D. Moyes, S. Yun, J. W. Fabre, M. Yacoub, and M. L. Rose
An Epithelial Cell Line That Can Stimulate Alloproliferation of Resting CD4+ T Cells, But Not After IFN-{gamma} Stimulation
J. Immunol., July 15, 2000; 165(2): 734 - 742.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. Madjdpour, B. Oertli, U. Ziegler, J. M. Bonvini, T. Pasch, and B. Beck-Schimmer
Lipopolysaccharide induces functional ICAM-1 expression in rat alveolar epithelial cells in vitro
Am J Physiol Lung Cell Mol Physiol, March 1, 2000; 278(3): L572 - L579.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Rosseau, J. Selhorst, K. Wiechmann, K. Leissner, U. Maus, K. Mayer, F. Grimminger, W. Seeger, and J. Lohmeyer
Monocyte Migration Through the Alveolar Epithelial Barrier: Adhesion Molecule Mechanisms and Impact of Chemokines
J. Immunol., January 1, 2000; 164(1): 427 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Hum Exp ToxicolHome page
C. D Lindsay and J. L Hambrook
Protection of A549 cells against the toxic effects of sulphur mustard by hexamethylenetetramine
Human and Experimental Toxicology, February 1, 1997; 16(2): 106 - 114.
[Abstract] [PDF]


© The Company of Biologists Ltd 1994