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Journal of Cell Science, Vol 107, Issue 2 543-552, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
CM Niessen, O Cremona, H Daams, S Ferraresi, A Sonnenberg and PC Marchisio
The Netherlands Cancer Institute, Division of Cell Biology, Amsterdam.
Integrin alpha 6 beta 4 is expressed in human peripheral nerves, but not in the central nervous system. This integrin heterodimer has previously been found in perineural fibroblast-like cells and in Schwann cells (SCs), which both assemble a basement membrane but do not form hemidesmosomes. We show here that in SCs, which had formed a myelin sheath, alpha 6 beta 4 was enriched in the proximity of the nucleus, at Ranvier paranodal areas and at Schmitt-Lanterman clefts; alpha 6 beta 4 was also found at the grooved interface between small axons and non-myelinating SCs. Immunoprecipitation of human peripheral nerves, in combination with Western blotting showed that beta 4 is associated with the alpha 6A subunit. Northern blot analysis of human peripheral nerves showed a single beta 4 transcript of 6 kb. Using the reverse transcriptase polymerase chain reaction, we detected two mRNA species, one for the most common (-70, -53) form of beta 4 and the other encoding the (+53) variant of beta 4. Cultured SCs were devoid of alpha 6 beta 4 but expressed alpha 6 beta 1, indicating that SCs lose beta 4 expression when contact with neurons is lost. Thus, resting SCs in contact with axons express alpha 6A in combination with beta 4, irrespective of myelin formation. We suggest that alpha 6 beta 4 expressed in SCs plays a role in peripheral neurogenesis.
This article has been cited by other articles:
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  C. E. E. M. Van der Zee, M. Kreft, G. Beckers, A. Kuipers, and A. Sonnenberg Conditional Deletion of the Itgb4 Integrin Gene in Schwann Cells Leads to Delayed Peripheral Nerve Regeneration J. Neurosci., October 29, 2008; 28(44): 11292 - 11303. [Abstract] [Full Text] [PDF]
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A. Nodari, S. C. Previtali, G. Dati, S. Occhi, F. A. Court, C. Colombelli, D. Zambroni, G. Dina, U. Del Carro, K. P. Campbell, et al. {alpha}6{beta}4 Integrin and Dystroglycan Cooperate to Stabilize the Myelin Sheath J. Neurosci., June 25, 2008; 28(26): 6714 - 6719. [Abstract] [Full Text] [PDF]
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 T. S. Hiran, J. E. Mazurkiewicz, P. Kreienberg, F. L. Rice, and S. E. LaFlamme Endothelial expression of the {alpha}6{beta}4 integrin is negatively regulated during angiogenesis J. Cell Sci., September 15, 2003; 116(18): 3771 - 3781. [Abstract] [Full Text] [PDF]
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L.-M. Chen, D. Bailey, and C. Fernandez-Valle Association of beta 1 Integrin with Focal Adhesion Kinase and Paxillin in Differentiating Schwann Cells J. Neurosci., May 15, 2000; 20(10): 3776 - 3784. [Abstract] [Full Text] [PDF]
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M. Nievers, I Kuikman, D Geerts, I. Leigh, and A Sonnenberg Formation of hemidesmosome-like structures in the absence of ligand binding by the (alpha)6(beta)4 integrin requires binding of HD1/plectin to the cytoplasmic domain of the (beta)4 integrin subunit J. Cell Sci., January 3, 2000; 113(6): 963 - 973. [Abstract] [PDF]
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 D. Geerts, L. Fontao, M. G. Nievers, R. Q.J. Schaapveld, P. E. Purkis, G. N. Wheeler, E. B. Lane, I. M. Leigh, and A. Sonnenberg Binding of Integrin {alpha}6{beta}4 to Plectin Prevents Plectin Association with F-Actin but Does Not Interfere with Intermediate Filament Binding J. Cell Biol., October 18, 1999; 147(2): 417 - 434. [Abstract] [Full Text] [PDF]
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R van der Neut, A. Cachaco, S Thorsteinsdottir, H Janssen, D Prins, J Bulthuis, M van der Valk, J Calafat, and A Sonnenberg Partial rescue of epithelial phenotype in integrin beta4 null mice by a keratin-5 promoter driven human integrin beta4 transgene J. Cell Sci., January 11, 1999; 112(22): 3911 - 3922. [Abstract] [PDF]
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M. Nievers, R. Schaapveld, L. Oomen, L Fontao, D Geerts, and A Sonnenberg Ligand-independent role of the beta 4 integrin subunit in the formation of hemidesmosomes J. Cell Sci., January 6, 1998; 111(12): 1659 - 1672. [Abstract] [PDF]
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 F. Saito, H. Yamada, Y. Sunada, H. Hori, T. Shimizu, and K. Matsumura Characterization of a 30-kDa Peripheral Nerve Glycoprotein That Binds Laminin and Heparin J. Biol. Chem., October 17, 1997; 272(42): 26708 - 26713. [Abstract] [Full Text] [PDF]
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L. Borradori, P. J. Koch, C. M. Niessen, S. Erkeland, M. R. v. Leusden, and A. Sonnenberg The Localization of Bullous Pemphigoid Antigen 180 (BP180) in Hemidesmosomes Is Mediated by Its Cytoplasmic Domain and Seems to be Regulated by the beta 4 Integrin Subunit J. Cell Biol., March 24, 1997; 136(6): 1333 - 1347. [Abstract] [Full Text] [PDF]
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C. Niessen, E. Hulsman, L. Oomen, I Kuikman, and A Sonnenberg A minimal region on the integrin beta4 subunit that is critical to its localization in hemidesmosomes regulates the distribution of HD1/plectin in COS-7 cells J. Cell Sci., January 8, 1997; 110(15): 1705 - 1716. [Abstract] [PDF]
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C. Fernandez-Valle, D. Gorman, A. M. Gomez, and M. B. Bunge Actin Plays a Role in Both Changes in Cell Shape and Gene- Expression Associated with Schwann Cell Myelination J. Neurosci., January 1, 1997; 17(1): 241 - 250. [Abstract] [Full Text] [PDF]
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 R Milner and C Ffrench-Constant A developmental analysis of oligodendroglial integrins in primary cells: changes in alpha v-associated beta subunits during differentiation Development, January 12, 1994; 120(12): 3497 - 3506. [Abstract] [PDF]
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M. Feltri, S. Scherer, R Nemni, J Kamholz, H Vogelbacker, M. Scott, N Canal, V Quaranta, and L Wrabetz Beta 4 integrin expression in myelinating Schwann cells is polarized, developmentally regulated and axonally dependent Development, January 5, 1994; 120(5): 1287 - 1301. [Abstract] [PDF]
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