spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kill, I. R.
Right arrow Articles by Shall, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kill, I. R.
Right arrow Articles by Shall, S.

Journal of Cell Science, Vol 107, Issue 2 571-579, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

The expression of proliferation-dependent antigens during the lifespan of normal and progeroid human fibroblasts in culture

IR Kill, RG Faragher, K Lawrence and S Shall
Department of Biological Sciences, The University, Dundee, UK.

Normal human fibroblasts display a limited lifespan in culture, which is due to a steadily decreasing fraction of cells that are able to proliferate. Using antibodies that react with antigens present in proliferating cells only, in an indirect immunofluorescence assay, we have estimated the fraction of proliferating cells in cultures of normal human fibroblasts. Furthermore, we have estimated the rate of decline in the fraction of proliferating cells during the process of cellular ageing by application of the assay to normal human fibroblasts throughout their lifespan in culture. Werner's Syndrome is an autosomal recessive disease in which individuals display symptoms of ageing prematurely. Werner's Syndrome fibroblasts display a reduced lifespan in culture compared with normal human fibroblasts. Like normal human fibroblasts, the growth of Werner's Syndrome fibroblasts is characterised by a decreasing fraction of cells reacting with the proliferation-associated antibodies throughout their lifespan in culture. However, the rate of loss of proliferating cells in Werner's Syndrome fibroblasts during the process of cellular ageing is accelerated 5- to 6-fold compared with the rate determined for normal human fibroblasts.


This article has been cited by other articles:


Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
A. B. Maier, I. L. Maier, D. van Heemst, and R. G. J. Westendorp
Colony Formation and Colony Size Do Not Reflect the Onset of Replicative Senescence in Human Fibroblasts
J. Gerontol. A Biol. Sci. Med. Sci., July 1, 2008; 63(7): 655 - 659.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. F. Passos, G. Saretzki, and T. von Zglinicki
DNA damage in telomeres and mitochondria during cellular senescence: is there a connection?
Nucleic Acids Res., December 3, 2007; 35(22): 7505 - 7513.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B. C. Capell, F. S. Collins, and E. G. Nabel
Mechanisms of Cardiovascular Disease in Accelerated Aging Syndromes
Circ. Res., July 6, 2007; 101(1): 13 - 26.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
T. Davis, D. M. Baird, M. F. Haughton, C. J. Jones, and D. Kipling
Prevention of Accelerated Cell Aging in Werner Syndrome Using a p38 Mitogen-Activated Protein Kinase Inhibitor
J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2005; 60(11): 1386 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. Kipling, T. Davis, E. L. Ostler, and R. G. A. Faragher
What Can Progeroid Syndromes Tell Us About Human Aging?
Science, September 3, 2004; 305(5689): 1426 - 1431.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Kikuchi, D. L. Harris, Y. Obara, T. Senoo, and N. C. Joyce
p27kip1 Antisense-Induced Proliferative Activity of Rat Corneal Endothelial Cells
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1763 - 1770.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Martin-Ruiz, G. Saretzki, J. Petrie, J. Ladhoff, J. Jeyapalan, W. Wei, J. Sedivy, and T. von Zglinicki
Stochastic Variation in Telomere Shortening Rate Causes Heterogeneity of Human Fibroblast Replicative Life Span
J. Biol. Chem., April 23, 2004; 279(17): 17826 - 17833.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Senoo, Y. Obara, and N. C. Joyce
EDTA: A Promoter of Proliferation in Human Corneal Endothelium
Invest. Ophthalmol. Vis. Sci., September 1, 2000; 41(10): 2930 - 2935.
[Abstract] [Full Text]

Home page
 IOVSHome page
T. Senoo and N. C. Joyce
Cell Cycle Kinetics in Corneal Endothelium from Old and Young Donors
Invest. Ophthalmol. Vis. Sci., March 1, 2000; 41(3): 660 - 667.
[Abstract] [Full Text]

Home page
 GeneticsHome page
L. Wang, C. E. Ogburn, C. B. Ware, W. C. Ladiges, H. Youssoufian, G. M. Martin, and J. Oshima
Cellular Werner Phenotypes in Mice Expressing a Putative Dominant-Negative Human WRN Gene
Genetics, January 1, 2000; 154(1): 357 - 362.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
I. Kill
Localisation of the Ki-67 antigen within the nucleolus. Evidence for a fibrillarin-deficient region of the dense fibrillar component
J. Cell Sci., January 6, 1996; 109(6): 1253 - 1263.
[Abstract] [PDF]


© The Company of Biologists Ltd 1994