|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 3 529-537, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
PA Johnston, A Stieber and NK Gonatas
Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
We have reported that MG160, an intrinsic membrane sialoglycoprotein of the Golgi apparatus (GA), resides in the medial cisternae of the organelle (Gonatas et al. (1989) J. Biol. Chem. 264, 646-653). In order to resolve the question whether MG160 acquires sialic acid residues in the trans cisternae or trans-Golgi network (TGN) prior to its retrograde transport, we have examined the effects of brefeldin A (BFA) on the post-translational processing of MG160, and the distribution of internalized wheat germ agglutinin covalently linked with HRP (WGA-HRP), which labels the TGN (Gonatas et al. (1977) J. Cell Biol. 73, 1-13). In BFA-treated PC12 cells, MG160 acquires resistance to endo H, but fails to be sialylated. This effect occurs in parallel with the redistribution of MG160 into an ER compartment dispersed throughout the cytoplasm including the nuclear envelope, and the collapse of the WGA-HRP-labelled TGN into vesicles and tubules surrounding the centriole. These results suggest that MG160 is not sialylated in BFA-treated cells because it is sequestered from the sialyltransferase enzyme(s), presumably located in the TGN, and provide evidence supporting the hypothesis for a retrograde transport pathway that recycles resident GA proteins, including MG160, between the Golgi cisternae and the TGN. To examine further the above hypothesis we studied cells treated with BFA and then allowed to recover from the effect of the drug for various lengths of time. After 15 minutes of recovery, cisternae of the Golgi apparatus, typically found in the pericentriolar region, are labeled by both MG160 and WGA-HRP.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
 |
|
  S. N. Zolov and V. V. Lupashin Cog3p depletion blocks vesicle-mediated Golgi retrograde trafficking in HeLa cells J. Cell Biol., February 28, 2005; 168(5): 747 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yoshino, B. M. Bieler, D. C. Harper, D. A. Cowan, S. Sutterwala, D. M. Gay, N. B. Cole, J. M. McCaffery, and M. S. Marks A role for GRIP domain proteins and/or their ligands in structure and function of the trans Golgi network J. Cell Sci., November 1, 2003; 116(21): 4441 - 4454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Lavoie, T. Meerloo, P. Lin, and M. G. Farquhar Calnuc, an EF-Hand Ca2+-Binding Protein, Is Stored and Processed in the Golgi and Secreted by the Constitutive-Like Pathway in AtT20 Cells Mol. Endocrinol., November 1, 2002; 16(11): 2462 - 2474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bachert, T. H. Lee, and A. D. Linstedt Lumenal Endosomal and Golgi-Retrieval Determinants Involved in pH-sensitive Targeting of an Early Golgi Protein Mol. Biol. Cell, October 1, 2001; 12(10): 3152 - 3160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Happe and P. Weidman Cell-free Transport to Distinct Golgi Cisternae Is Compartment Specific and ARF Independent J. Cell Biol., February 9, 1998; 140(3): 511 - 523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. Cole, J. Ellenberg, J. Song, D. DiEuliis, and J. Lippincott-Schwartz Retrograde Transport of Golgi-localized Proteins to the ER J. Cell Biol., January 12, 1998; 140(1): 1 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gonatas, Y. Chen, A Stieber, Z Mourelatos, and N. Gonatas Truncations of the C-terminal cytoplasmic domain of MG160, a medial Golgi sialoglycoprotein, result in its partial transport to the plasma membrane and filopodia J. Cell Sci., January 1, 1998; 111(2): 249 - 260. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Johannes, D. Tenza, C. Antony, and B. Goud Retrograde Transport of KDEL-bearing B-fragment of Shiga Toxin J. Biol. Chem., August 1, 1997; 272(31): 19554 - 19561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Martinez, C. Antony, G. Pehau-Arnaudet, E. G. Berger, J. Salamero, and B. Goud GTP-bound forms of rab6 induce the redistribution of Golgi proteins into the endoplasmic reticulum PNAS, March 4, 1997; 94(5): 1828 - 1833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Hoe, P. Slusarewicz, T. Misteli, R. Watson, and G. Warren Evidence for Recycling of the Resident medial/trans Golgi Enzyme, N-Acetylglucosaminyltransferase I, in ldlD Cells J. Biol. Chem., October 20, 1995; 270(42): 25057 - 25063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Rabouille, N Hui, F Hunte, R Kieckbusch, E. Berger, G Warren, and T Nilsson Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides J. Cell Sci., January 4, 1995; 108(4): 1617 - 1627. [Abstract] [PDF]
|
|
