|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 3 727-736, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
EC Davis
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
In the developing aorta, endothelial cell connecting filaments extend from the abluminal surface of the endothelial cell to the subjacent elastic lamina. The connecting filaments are in alignment with intracellular stress fibers and are oriented parallel to the direction of blood flow. In the present study, the composition of the endothelial cell connecting filaments was investigated by indirect immunogold labeling with antibodies to the microfibril proteins, MP340 (fibrillin) and MAGP, and to fibronectin and heparan sulfate proteoglycan (HSPG). In the subendothelial matrix of both 15-day gestational and 5-day post-natal mouse aortae, the connecting filaments showed moderate immunoreactivity with anti-MP340; however, no significant immunoreaction was seen with anti-MAGP. Anti-fibronectin strongly labeled the connecting filaments and a weak immunoreaction was seen with anti-HSPG. In contrast, the adjacent 'elastin-associated microfibrils' showed a very strong immunoreaction with anti-MP340 and a moderate reaction with anti-MAGP. Little or no reaction was seen with anti-fibronectin or anti-HSPG. The filaments that connect endothelial cells to the subjacent elastic lamina during aortic development are thus microfibrillar in nature and related to elastin-associated microfibrils as evidenced by their positive immunoreaction with anti-MP340. The absence of labeling with anti-MAGP, however, suggests that either these fibrillin-containing filaments do not contain MAGP or that the immunoreactive epitopes are blocked by the proteins that coat the connecting filaments such as fibronectin. These results suggest that microfibrils not in association with elastin may play a role in cell anchorage and, more specifically, in the aorta may be involved in maintaining the structural integrity of the endothelial cell layer during early development of the vessel wall. Furthermore, the absence of immunoreactivity with anti-MAGP on the fibrillin-containing endothelial cell connecting filaments raises the possibility that microfibrils may consist of a family of related filaments rather than a single structural entity.
This article has been cited by other articles:
|
|
 |
|
  L. Carta, L. Pereira, E. Arteaga-Solis, S. Y. Lee-Arteaga, B. Lenart, B. Starcher, C. A. Merkel, M. Sukoyan, A. Kerkis, N. Hazeki, et al. Fibrillins 1 and 2 Perform Partially Overlapping Functions during Aortic Development J. Biol. Chem., March 24, 2006; 281(12): 8016 - 8023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Safar and H. S. Boudier Vascular Development, Pulse Pressure, and the Mechanisms of Hypertension Hypertension, July 1, 2005; 46(1): 205 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tiedemann, T. Sasaki, E. Gustafsson, W. Gohring, B. Batge, H. Notbohm, R. Timpl, T. Wedel, U. Schlotzer-Schrehardt, and D. P. Reinhardt Microfibrils at Basement Membrane Zones Interact with Perlecan via Fibrillin-1 J. Biol. Chem., March 25, 2005; 280(12): 11404 - 11412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Werneck, B. C. Trask, T. J. Broekelmann, T. M. Trask, T. M. Ritty, F. Segade, and R. P. Mecham Identification of a Major Microfibril-associated Glycoprotein-1-binding Domain in Fibrillin-2 J. Biol. Chem., May 28, 2004; 279(22): 23045 - 23051. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W.M. Fedak, M. P.L. de Sa, S. Verma, N. Nili, P. Kazemian, J. Butany, B. H. Strauss, R. D. Weisel, and T. E. David Vascular matrix remodeling in patients with bicuspid aortic valve malformations: implications for aortic dilatation J. Thorac. Cardiovasc. Surg., September 1, 2003; 126(3): 797 - 805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Qian, M. Li, Y. Cai, C. J. Ward, S. Somlo, P. C. Harris, and V. E. Torres Analysis of the Polycystins in Aortic Vascular Smooth Muscle Cells J. Am. Soc. Nephrol., September 1, 2003; 14(9): 2280 - 2287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Segade, B. C. Trask, T. J. Broekelmann, R. A. Pierce, and R. P. Mecham Identification of a Matrix-binding Domain in MAGP1 and MAGP2 and Intracellular Localization of Alternative Splice Forms J. Biol. Chem., March 22, 2002; 277(13): 11050 - 11057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sinha, A. M. Heagerty, C.A. Shuttleworth, and C. M. Kielty Expression of latent TGF-beta binding proteins and association with TGF-beta1 and fibrillin-1 following arterial injury Cardiovasc Res, March 1, 2002; 53(4): 971 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Bunton, N. J. Biery, L. Myers, B. Gayraud, F. Ramirez, and H. C. Dietz Phenotypic Alteration of Vascular Smooth Muscle Cells Precedes Elastolysis in a Mouse Model of Marfan Syndrome Circ. Res., January 19, 2001; 88(1): 37 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. W. Robb, H. Wachi, T. Schaub, R. P. Mecham, and E. C. Davis Characterization of an In Vitro Model of Elastic Fiber Assembly Mol. Biol. Cell, November 1, 1999; 10(11): 3595 - 3605. [Abstract] [Full Text]
|
|
|
|
|
|
M. Hyytiainen, J. Taipale, C.-H. Heldin, and J. Keski-Oja Recombinant Latent Transforming Growth Factor beta -binding Protein 2 Assembles to Fibroblast Extracellular Matrix and Is Susceptible to Proteolytic Processing and Release J. Biol. Chem., August 7, 1998; 273(32): 20669 - 20676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sakamoto, T. Broekelmann, D. A. Cheresh, F. Ramirez, J. Rosenbloom, and R. P. Mecham Cell-type Specific Recognition of RGD- and Non-RGD-containing Cell Binding Domains in Fibrillin-1 J. Biol. Chem., March 1, 1996; 271(9): 4916 - 4922. [Abstract] [Full Text] [PDF]
|
|
