|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 4 1031-1040, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
R Chammas, D Taverna, N Cella, C Santos and NE Hynes
Ludwig Institute for Cancer Research, Sao Paulo, Brazil.
HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show that competency for differentiation is due at least in part to the modulation of extracellular matrix components. In particular we have studied laminin and tenascin. EGF alters endogenous laminin assembly. In addition, promotion of competency can be partially mimicked by plating HC11 cells on the E8 laminin fragment, which is able to induce lactogenic responsiveness in cells grown in the absence of EGF or bFGF. The production and assembly of tenascin is also dependent upon the growth conditions of the HC11 cells. EGF- or bFGF-grown competent cells produce tenascin but do not assemble it at the extracellular matrix as efficiently as Ins- or PDGF-grown, non-competent cells. This alteration apparently leads to a change in the cellular microenvironment that supports beta-casein production. In addition, when competent cells are plated on dishes coated with tenascin, lactogenic hormone induction of beta-casein is inhibited. The data suggest that tenascin assembly and beta-casein production are opposing features of a coordinated differentiation program of HC11 cells.
This article has been cited by other articles:
|
|
 |
|
  J. Beattie, K. Phillips, J. H Shand, M. Szymanowska, D. J Flint, and G. J Allan Molecular recognition characteristics in the insulin-like growth factor (IGF)-insulin-like growth factor binding protein -3/5 (IGFBP-3/5) heparin axis J. Mol. Endocrinol., February 1, 2005; 34(1): 163 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L Hebbard, A Steffen, V Zawadzki, C Fieber, N Howells, J Moll, H Ponta, M Hofmann, and J Sleeman CD44 expression and regulation during mammary gland development and function J. Cell Sci., January 7, 2000; 113(14): 2619 - 2630. [Abstract] [PDF]
|
|
|
|
|
|
P Schedin, R Strange, T Mitrenga, P Wolfe, and M Kaeck Fibronectin fragments induce MMP activity in mouse mammary epithelial cells: evidence for a role in mammary tissue remodeling J. Cell Sci., January 3, 2000; 113(5): 795 - 806. [Abstract] [PDF]
|
|
|
|
|
|
P Pujuguet, M Simian, J Liaw, R Timpl, Z Werb, and M. Bissell Nidogen-1 regulates laminin-1-dependent mammary-specific gene expression J. Cell Sci., January 3, 2000; 113(5): 849 - 858. [Abstract] [PDF]
|
|
|
|
 |
|
 M. E. Benton, K.-S. Chen, J. D. Haag, C. A. Sattler, and M. N. Gould Precocious Differentiation of the Virgin Wistar-Kyoto Rat Mammary Gland Endocrinology, June 1, 1999; 140(6): 2659 - 2671. [Abstract] [Full Text]
|
|
|
|
|
|
J. Talts, G Wirl, M Dictor, W. Muller, and R Fassler Tenascin-C modulates tumor stroma and monocyte/macrophage recruitment but not tumor growth or metastasis in a mouse strain with spontaneous mammary cancer J. Cell Sci., January 6, 1999; 112(12): 1855 - 1864. [Abstract] [PDF]
|
|
|
|
|
|
M. Deugnier, M. Faraldo, P Rousselle, J. Thiery, and M. Glukhova Cell-extracellular matrix interactions and EGF are important regulators of the basal mammary epithelial cell phenotype J. Cell Sci., January 4, 1999; 112(7): 1035 - 1044. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Neve, C. Chang, G. K. Scott, A. Wong, R. R. Friis, N. E. Hynes, and C. C. Benz The epithelium-specific Ets transcription factor ESX is associated with mammary gland development and involution FASEB J, November 1, 1998; 12(14): 1541 - 1550. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Thomasset, A. Lochter, C. J. Sympson, L. R. Lund, D. R. Williams, O. Behrendtsen, Z. Werb, and M. J. Bissell Expression of Autoactivated Stromelysin-1 in Mammary Glands of Transgenic Mice Leads to a Reactive Stroma During Early Development Am. J. Pathol., August 1, 1998; 153(2): 457 - 467. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Cella, B. Groner, and N. E. Hynes Characterization of Stat5a and Stat5b Homodimers and Heterodimers and Their Association with the Glucocortiocoid Receptor in Mammary Cells Mol. Cell. Biol., April 1, 1998; 18(4): 1783 - 1792. [Abstract] [Full Text]
|
|
|
|
|
|
D Alford, D Baeckstrom, M Geyp, P Pitha, and J Taylor-Papadimitriou Integrin-matrix interactions affect the form of the structures developing from human mammary epithelial cells in collagen or fibrin gels J. Cell Sci., January 2, 1998; 111(4): 521 - 532. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Wartmann, N. Cella, P. Hofer, B. Groner, X. Liu, L. Hennighausen, and N. E. Hynes Lactogenic Hormone Activation of Stat5 and Transcription of the beta -Casein Gene in Mammary Epithelial Cells Is Independent of p42 ERK2 Mitogen-activated Protein Kinase Activity J. Biol. Chem., December 13, 1996; 271(50): 31863 - 31868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Wirl, M Hermann, P Ekblom, and R Fassler Mammary epithelial cell differentiation in vitro is regulated by an interplay of EGF action and tenascin-C downregulation J. Cell Sci., January 6, 1995; 108(6): 2445 - 2456. [Abstract] [PDF]
|
|
|
|
|
|
P. Jones, N Boudreau, C. Myers, H. Erickson, and M. Bissell Tenascin-C inhibits extracellular matrix-dependent gene expression in mammary epithelial cells. Localization of active regions using recombinant tenascin fragments J. Cell Sci., January 2, 1995; 108(2): 519 - 527. [Abstract] [PDF]
|
|
