spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dover, R.
Right arrow Articles by Chinery, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dover, R.
Right arrow Articles by Chinery, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 107, Issue 5 1181-1184, Copyright © 1994 by Company of Biologists

JOURNAL ARTICLES

p53 expression in cultured cells following radioisotope labelling

R Dover, Y Jayaram, K Patel and R Chinery
Department of Anatomy, Queen Mary and Westfield College, University of London, UK.

p53 inhibits division following cellular damage. Cultured cells were found to express p53 protein following pulse labelling with radioisotopes, even at low doses normally used for growth and metabolic labelling studies. Some stem cells are exquisitely sensitive to radiation and thus p53 may have evolved as a major regulator of stem cell function. Therefore any genetic damage may be able to induce p53 expression, which in turn will affect the biochemical outcome of many experiments by both cell cycle arrest and other mechanisms. In some cases the use of radioisotopes may directly change the results of the experiment. This will require a careful re-evaluation of the current literature and experimental protocols utilising radioisotopes.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 FASEB J.Home page
N. F. MARKO, P. B. DIEFFENBACH, G. YAN, S. CERYAK, R. W. HOWELL, T. A. MCCAFFREY, and V. W. HU
Does metabolic radiolabeling stimulate the stress response? Gene expression profiling reveals differential cellular responses to internal beta vs. external gamma radiation
FASEB J, August 1, 2003; 17(11): 1470 - 1486.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
V. W. HU, D. S. HEIKKA, P. B. DIEFFENBACH, and L. HA
Metabolic radiolabeling: experimental tool or Trojan horse? 35S-Methionine induces DNA fragmentation and p53-dependent ROS production
FASEB J, July 1, 2001; 15(9): 1562 - 1568.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
V. W. HU and D. S. HEIKKA
Radiolabeling revisited: metabolic labeling with 35S-methionine inhibits cell cycle progression, proliferation, and survival
FASEB J, March 1, 2000; 14(3): 448 - 454.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H.-H. Wu and J. Momand
Pyrrolidine Dithiocarbamate Prevents p53 Activation and Promotes p53 Cysteine Residue Oxidation
J. Biol. Chem., July 24, 1998; 273(30): 18898 - 18905.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
R C FITZGERALD and T R HUPP
Stress protein response in human oesophageal epithelium may be influenced by the ex vivo culture technique • Reply
Gut, February 1, 1998; 42(2): 307 - 308.
[Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. M. Milne, L. E. Campbell, D. G. Campbell, and D. W. Meek
p53 Is Phosphorylated in Vitro and in Vivo by an Ultraviolet Radiation-induced Protein Kinase Characteristic of the c-Jun Kinase, JNK1
J. Biol. Chem., March 10, 1995; 270(10): 5511 - 5518.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Higashimoto, S.'i. Saito, X.-H. Tong, A. Hong, K. Sakaguchi, E. Appella, and C. W. Anderson
Human p53 Is Phosphorylated on Serines 6 and 9 in Response to DNA Damage-inducing Agents
J. Biol. Chem., July 21, 2000; 275(30): 23199 - 23203.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Isaacs, S.'i. Saito, and L. M. Neckers
Requirement for HDM2 Activity in the Rapid Degradation of p53 in Neuroblastoma
J. Biol. Chem., May 18, 2001; 276(21): 18497 - 18506.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1994