|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 5 1321-1331, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
M Mizuno and SJ Singer
Department of Biology, University of California at San Diego, La Jolla 92037.
The intracellular transport of secretory proteins involves at an early stage the formation of vesicles from transitional elements of the endoplasmic reticulum (ER) containing these proteins and the transfer of these vesicles to the cis-face of the Golgi apparatus. We propose that the latter transfer process does not occur by random diffusion, but is instead mediated by tracking along stable microtubules. To test this proposal, we have carried out double immunoelectron microscopic labeling experiments on frozen sections of HepG2 hepatoma cells secreting the protein human serum albumin (HSA). By a cycloheximide treatment protocol, the stage during which the transfer of newly synthesized HSA from the ER to the Golgi apparatus occurs in vivo was determined. Sections of the cells were then double immunolabeled using primary antibodies to HSA and to glu-tubulin, the latter specifically detecting stable microtubules. We observed a significantly high frequency of HSA-containing structures between the ER and the Golgi apparatus with which stable microtubules were closely associated. These results support the proposal that stable microtubules may play a critical role in directing the transfer process from the ER to the Golgi apparatus.
This article has been cited by other articles:
|
|
 |
|
  R. Sannerud, M. Marie, C. Nizak, H. A. Dale, K. Pernet-Gallay, F. Perez, B. Goud, and J. Saraste Rab1 Defines a Novel Pathway Connecting the Pre-Golgi Intermediate Compartment with the Cell Periphery Mol. Biol. Cell, April 1, 2006; 17(4): 1514 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Phung-Koskas, A. Pilon, C. Pous, C. Betzina, M. Sturm, M.-L. Bourguet-Kondracki, G. Durand, and A. Drechou STAT5B-mediated Growth Hormone Signaling Is Organized by Highly Dynamic Microtubules in Hepatic Cells J. Biol. Chem., January 14, 2005; 280(2): 1123 - 1131. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Stephens and R. Pepperkok Differential effects of a GTP-restricted mutant of Sar1p on segregation of cargo during export from the endoplasmic reticulum J. Cell Sci., July 15, 2004; 117(16): 3635 - 3644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Chabin-Brion, J. Marceiller, F. Perez, C. Settegrana, A. Drechou, G. Durand, and C. Pous The Golgi Complex Is a Microtubule-organizing Organelle Mol. Biol. Cell, July 1, 2001; 12(7): 2047 - 2060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pagano, F. Letourneur, D. Garcia-Estefania, J.-L. Carpentier, L. Orci, and J.-P. Paccaud Sec24 Proteins and Sorting at the Endoplasmic Reticulum J. Biol. Chem., March 19, 1999; 274(12): 7833 - 7840. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D Toomre, P Keller, J White, J. Olivo, and K Simons Dual-color visualization of trans-Golgi network to plasma membrane traffic along microtubules in living cells J. Cell Sci., January 1, 1999; 112(1): 21 - 33. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Pous, K. Chabin, A. Drechou, L. Barbot, T. Phung-Koskas, C. Settegrana, M.L. Bourguet-Kondracki, M. Maurice, D. Cassio, M. Guyot, et al. Functional Specialization of Stable and Dynamic Microtubules in Protein Traffic in WIF-B Cells J. Cell Biol., July 13, 1998; 142(1): 153 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Minin Dispersal of Golgi apparatus in nocodazole-treated fibroblasts is a kinesin-driven process J. Cell Sci., January 10, 1997; 110(19): 2495 - 2505. [Abstract] [PDF]
|
|
|
|
|
|
A. Blocker, F. F. Severin, A. Habermann, A. A. Hyman, G. Griffiths, and J. K. Burkhardt Microtubule-associated Protein-dependent Binding of Phagosomes to Microtubules J. Biol. Chem., February 16, 1996; 271(7): 3803 - 3811. [Abstract] [Full Text] [PDF]
|
|
