|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 7 1783-1796, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
MC Tiveron, M Nosten-Bertrand, H Jani, D Garnett, EM Hirst, F Grosveld and RJ Morris
Laboratory of Neurobiology (Norman and Sadie Lee Centre), National Institute for Medical Research, Mill Hill, London, UK.
The surface glycoprotein, Thy-1, when expressed by transfection in NG115/401L neural cells, inhibits their neurite outgrowth over astrocytes. We have investigated the role of the glycosylphosphatidylinositol anchor of Thy-1 in this inhibition. Hybrid molecules, in which the lipid anchor was replaced by polypeptide transmembrane domains, were expressed by transfection. Lines expressing Thy-1 with the transmembrane and full cytoplasmic domains of NCAM-140, or with the transmembrane and truncated cytoplasmic domain of CD8, were not inhibited in their ability to extend neurites over astrocytes. Truncation of the cytoplasmic domain of NCAM-140 to just two amino acids, however, produced a transmembrane form of Thy-1 that, when expressed at high levels, inhibited neurite outgrowth. All forms of Thy-1 were concentrated in clusters that occurred primarily on fine filopodia. In double transfectants expressing normal Thy-1 and Thy-1 with the full NCAM cytoplasmic tail, the clusters of each form were separate, with no instances of the transmembrane form being found within the clusters of lipid-anchored Thy-1. Thy-1 with the two-amino-acid cytoplasmic domain of NCAM also occurred in clusters separate from those occupied by lipid-anchored Thy-1, but substantial 'invasion' of the clusters of normal Thy-1 by this transmembrane construct occurred. We suggest that the ability of this hybrid protein to enter the lipid-anchored clusters enables it to activate the signalling pathways that normal Thy-1 uses. Thus the membrane anchor, in targetting Thy-1 to different microdomains on the cell surface, determines its ability to inhibit neurite outgrowth on astrocytes.
This article has been cited by other articles:
|
|
 |
|
  T. A. Rege and J. S. Hagood Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis FASEB J, June 1, 2006; 20(8): 1045 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Sarnataro, V. Campana, S. Paladino, M. Stornaiuolo, L. Nitsch, and C. Zurzolo PrPC Association with Lipid Rafts in the Early Secretory Pathway Stabilizes Its Cellular Conformation Mol. Biol. Cell, September 1, 2004; 15(9): 4031 - 4042. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Barker, M. A. Pallero, M. W. MacEwen, S. G. Tilden, A. Woods, J. E. Murphy-Ullrich, and J. S. Hagood Thrombospondin-1-induced Focal Adhesion Disassembly in Fibroblasts Requires Thy-1 Surface Expression, Lipid Raft Integrity, and Src Activation J. Biol. Chem., May 28, 2004; 279(22): 23510 - 23516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Hagood, P. J. Miller, J. A. Lasky, A. Tousson, B. Guo, G. M. Fuller, and J. C. McIntosh Differential expression of platelet-derived growth factor-alpha receptor by Thy-1- and Thy-1+ lung fibroblasts Am J Physiol Lung Cell Mol Physiol, July 1, 1999; 277(1): L218 - L224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-J. Jeng, S. A. McCarroll, T. F. J. Martin, E. Floor, J. Adams, D. Krantz, S. Butz, R. Edwards, and E. S. Schweitzer Thy-1 Is a Component Common to Multiple Populations of Synaptic Vesicles J. Cell Biol., February 9, 1998; 140(3): 685 - 698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Barboni, B. Rivero, A. George, S. Martin, D. Renoup, E. Hounsell, P. Barber, and R. Morris The glycophosphatidylinositol anchor affects the conformation of Thy-1 protein J. Cell Sci., January 2, 1995; 108(2): 487 - 497. [Abstract] [PDF]
|
|
