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Journal of Cell Science, Vol 107, Issue 8 2279-2284, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
LM Coluccio
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322.
We have previously purified and characterized two myosin-1 isoforms from rat liver (molecular masses 130 kDa and 110 kDa; L. M. Coluccio and C. Conaty (1993) Cell Motil. Cytoskel. 24, 189-199). Here, we describe the purification and characterization from liver of a third myosin-1 (molecular mass 105 kDa) and determine the number of calmodulin molecules associated with each of these three myosin-1 isoforms. The 105 kDa polypeptide, solubilized from liver homogenates with the addition of ATP, co-sediments with F-actin, co-purifies with calmodulin, and binds calmodulin in the presence of EGTA. Antibodies directed against chicken intestinal brush border myosin-1 cross-react with the 105 kDa polypeptide on immunoblots. Partial peptide sequence analysis indicates that the polypeptide corresponds with an MM1 gamma gene product that represents a myosin-1 isoform cloned from mouse brain (Sherr et al. (1993) J. Cell Biol. 120, 1405-1416). A comparison of calmodulin binding to the now three isolated forms of myosin-1 in liver shows that in solution the 105 kDa and 110 kDa polypeptides bind two molecules of calmodulin each whereas the 130 kDa binds six molecules of calmodulin.
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  W. F. Stafford, M. L. Walker, J. A. Trinick, and L. M. Coluccio Mammalian Class I Myosin, Myo1b, Is Monomeric and Cross-Links Actin Filaments as Determined by Hydrodynamic Studies and Electron Microscopy Biophys. J., January 1, 2005; 88(1): 384 - 391. [Abstract] [Full Text] [PDF]
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 O. Sato, H. D. White, A. Inoue, B. Belknap, R. Ikebe, and M. Ikebe Human Deafness Mutation of Myosin VI (C442Y) Accelerates the ADP Dissociation Rate J. Biol. Chem., July 9, 2004; 279(28): 28844 - 28854. [Abstract] [Full Text] [PDF]
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P. G. Gillespie, S. K. H. Gillespie, J. A. Mercer, K. Shah, and K. M. Shokat Engineering of the Myosin-Ibeta Nucleotide-binding Pocket to Create Selective Sensitivity to N6-modified ADP Analogs J. Biol. Chem., October 29, 1999; 274(44): 31373 - 31381. [Abstract] [Full Text] [PDF]
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L. M. Coluccio and M. A. Geeves Transient Kinetic Analysis of the 130-kDa Myosin I (MYR-1 Gene Product) from Rat Liver. A MYOSIN I DESIGNED FOR MAINTENANCE OF TENSION? J. Biol. Chem., July 30, 1999; 274(31): 21575 - 21580. [Abstract] [Full Text] [PDF]
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T. Zhu, K. Beckingham, and M. Ikebe High Affinity Ca2+ Binding Sites of Calmodulin Are Critical for the Regulation of Myosin Ibeta Motor Function J. Biol. Chem., August 7, 1998; 273(32): 20481 - 20486. [Abstract] [Full Text] [PDF]
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 C Ruppert, J Godel, R. Muller, R Kroschewski, J Reinhard, and M Bahler Localization of the rat myosin I molecules myr 1 and myr 2 and in vivo targeting of their tail domains J. Cell Sci., January 12, 1995; 108(12): 3775 - 3786. [Abstract] [PDF]
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M. A. Geeves, C. Perreault-Micale, and L. M. Coluccio Kinetic Analyses of a Truncated Mammalian Myosin I Suggest a Novel Isomerization Event Preceding Nucleotide Binding J. Biol. Chem., July 7, 2000; 275(28): 21624 - 21630. [Abstract] [Full Text] [PDF]
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C. Perreault-Micale, A. D. Shushan, and L. M. Coluccio Truncation of a Mammalian Myosin I Results in Loss of Ca2+-sensitive Motility J. Biol. Chem., July 7, 2000; 275(28): 21618 - 21623. [Abstract] [Full Text] [PDF]
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