|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 8 2285-2289, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
TL Tuan, LC Keller, D Sun, ME Nimni and D Cheung
Department of Surgery, Childrens Hospital Los Angeles, California.
The effects of dermal fibroblasts on keratinocyte outgrowth on collagen substrata was studied using an in vitro keratinocyte-collagen gel composite model. Skin fibroblasts were seeded inside collagen gels, which remained attached to the cell culture plastic substratum. Fibroblasts incorporated in collagen gels were either kept viable throughout the study, or were lysed hypotonically with water at different time intervals (2 hours and 5 days). Results show that very little keratinocyte outgrowth occurred on either plain collagen gels or gels that had previously contained viable fibroblasts for 2 hours. A 3- to 4-fold increase in keratinocyte outgrowth occurred on collagen gels that had previously contained viable fibroblasts for 5 days. A striking increase (20-fold) in keratinocyte outgrowth was observed on collagen gels that contain viable fibroblasts. The effect of fibroblast diffusible factors on keratinocyte outgrowth was further studied with a co-culture system using Millicell inserts. It was found that the co-culture of fibroblasts with the composite enhanced keratinocyte outgrowth on collagen gels that had previously contained viable fibroblasts for 5 days. Among all, however, the keratinocyte outgrowth was far better on gels containing viable fibroblasts. Addition of keratinocyte growth factor or its neutralizing antibody did not affect keratinocyte outgrowth. These results suggest that dermal fibroblasts can activate keratinocyte outgrowth on collagen matrices through some diffusible factors other than keratinocyte growth factor, and epithelial-mesenchymal interactions exert some special effects on keratinocyte outgrowth on collagen gels.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Florin, N. Maas-Szabowski, S. Werner, A. Szabowski, and P. Angel Increased keratinocyte proliferation by JUN-dependent expression of PTN and SDF-1 in fibroblasts J. Cell Sci., May 1, 2005; 118(9): 1981 - 1989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-L. Tuan, H. Wu, E. Y. Huang, S. S. N. Chong, W. Laug, D. Messadi, P. Kelly, and A. Le Increased Plasminogen Activator Inhibitor-1 in Keloid Fibroblasts May Account for their Elevated Collagen Accumulation in Fibrin Gel Cultures Am. J. Pathol., May 1, 2003; 162(5): 1579 - 1589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-P. Han, Y.-D. Nien, and W. L. Garner Tumor Necrosis Factor-alpha -induced Proteolytic Activation of Pro-matrix Metalloproteinase-9 by Human Skin Is Controlled by Down-regulating Tissue Inhibitor of Metalloproteinase-1 and Mediated by Tissue-associated Chymotrypsin-like Proteinase J. Biol. Chem., July 19, 2002; 277(30): 27319 - 27327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Han, T. Tuan, H Wu, M Hughes, and W. Garner TNF-alpha stimulates activation of pro-MMP2 in human skin through NF-(kappa)B mediated induction of MT1-MMP J. Cell Sci., January 1, 2001; 114(1): 131 - 139. [Abstract] [PDF]
|
|
|
|
 |
|
 K. Izumi, H. Terashi, C.L. Marcelo, and S.E. Feinberg Development and Characterization of a Tissue-engineered Human Oral Mucosa Equivalent Produced in a Serum-free Culture System Journal of Dental Research, March 1, 2000; 79(3): 798 - 805. [Abstract] [PDF]
|
|
