|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 107, Issue 8 2323-2333, Copyright © 1994 by Company of Biologists
JOURNAL ARTICLES |
P Pesheva, R Probstmeier, AP Skubitz, JB McCarthy, LT Furcht and M Schachner
Department of Neurobiology, Swiss Federal Institute of Technology, Zurich.
Cell adhesion and neurite outgrowth on fibronectin is a multistep process modulated by different extra- and intracellular signals. Fibronectin-mediated cell attachment and spreading can be affected in a negative way by tenascin-C, an extracellular matrix glycoprotein expressed in a temporally and spacially restricted manner during early morphogenesis. Tenascin-R (J1-160/180), consisting of two major isoforms of 160 kDa (tenascin-R 160) and 180 kDa (tenascin-R 180) in mammals, is an extracellular matrix glycoprotein of the central nervous system that shares high structural homologies with tenascin-C. Here we show that in relation to fibronectin-mediated adhesion, the two extracellular matrix molecules are also functionally closely related. When offered as mixed substrata with other extracellular matrix molecules, the two tenascin-R isoforms and tenascin-C derived from mouse brain selectively inhibit fibronectin-dependent cell adhesion and neurite outgrowth, and affect cell morphology of different mesenchymal and neural cells. This effect is partially due to interactions at the substrate level that result in a steric hindrance and/or conformational change of the cell binding sites of the fibronectin molecule. In addition, tenascin-R 180 and tenascin-C interact with cells by an RGD- and beta 1 integrin-independent mechanism, leading to cell rounding and detachment from such substrata. The expression of tenascin-R and tenascin-C in the nervous system at times and locations where fibronectin-mediated cellular processes take place may be related to the role of inhibitory signals in the extracellular matrix in the regulation of cell migration and differentiation in general.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Liao, W. Huang, M. Schachner, Y. Guan, J. Guo, J. Yan, J. Qin, X. Bai, and L. Zhang {beta} 1 Integrin-mediated Effects of Tenascin-R Domains EGFL and FN6-8 on Neural Stem/Progenitor Cell Proliferation and Differentiation in Vitro J. Biol. Chem., October 10, 2008; 283(41): 27927 - 27936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Woodworth, P. Pesheva, D. Fiete, and J. U. Baenziger Neuronal-specific Synthesis and Glycosylation of Tenascin-R J. Biol. Chem., March 12, 2004; 279(11): 10413 - 10421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Huang, R. Chiquet-Ehrismann, J. V. Moyano, A. Garcia-Pardo, and G. Orend Interference of Tenascin-C with Syndecan-4 Binding to Fibronectin Blocks Cell Adhesion and Stimulates Tumor Cell Proliferation Cancer Res., December 1, 2001; 61(23): 8586 - 8594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Garcion, A. Faissner, and C. ffrench-Constant Knockout mice reveal a contribution of the extracellular matrix molecule tenascin-C to neural precursor proliferation and migration Development, July 1, 2001; 128(13): 2485 - 2496. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Becker, T. Becker, R. L. Meyer, and M. Schachner Tenascin-R Inhibits the Growth of Optic Fibers In Vitro But Is Rapidly Eliminated during Nerve Regeneration in the Salamander Pleurodeles waltl J. Neurosci., January 15, 1999; 19(2): 813 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. David, J. Orpiszewski, X.-C. Zhu, K. J. Reissner, and D. W. Aswad Isoaspartate in Chrondroitin Sulfate Proteoglycans of Mammalian Brain J. Biol. Chem., November 27, 1998; 273(48): 32063 - 32070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Angelov, M. Walther, M. Streppel, O. Guntinas-Lichius, W. F. Neiss, R. Probstmeier, and P. Pesheva Tenascin-R Is Antiadhesive for Activated Microglia that Induce Downregulation of the Protein after Peripheral Nerve Injury: a New Role in Neuronal Protection J. Neurosci., August 15, 1998; 18(16): 6218 - 6229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pierre, G. Rougon, M. Allard, R. Bonhomme, G. Gennarini, D. A. Poulain, and Dionysia. T. Theodosis Regulated Expression of the Cell Adhesion Glycoprotein F3 in Adult Hypothalamic Magnocellular Neurons J. Neurosci., July 15, 1998; 18(14): 5333 - 5343. [Abstract] [Full Text] [PDF]
|
|
