|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 1 333-342, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
CE Turner, KM Pietras, DS Taylor and CJ Molloy
Department of Anatomy and Cell Biology, SUNY Health Science Center, Syracuse 13210, USA.
Angiotensin II is a potent vasoconstrictor that has been also implicated in vascular hyperproliferative diseases, including atherosclerosis and restenosis following angioplasty. Treatment of cultured, serum-starved rat aortic smooth muscle cells with angiotensin II causes rapid protein tyrosine phosphorylation that precedes cell mitogenesis. We have identified two of the phosphoproteins as paxillin (75 kilodaltons) and the tyrosine kinase pp125Fak, both components of actin-associated focal adhesion sites. Angiotensin II stimulated a 5-fold increase in the tyrosine phosphorylation of paxillin and a smaller (1.5-fold) increase in pp125Fak tyrosine phosphorylation. Paxillin tyrosine phosphorylation was evident within 1 minute, and was maximal after 10 minutes. Similar elevated protein tyrosine phosphorylation levels of paxillin were obtained with exposure of the rat aortic smooth muscle cells to peptides endothelin-1 and alpha-thrombin that function, as angiotensin II, through binding to members of the seven transmembrane domain G protein coupled receptors. Angiotensin II treatment also stimulated the production of a well-ordered actin-containing stress fiber network and prominent paxillin-containing focal adhesions. The focal adhesions stained intensely with anti-phosphotyrosine antibody suggesting the tyrosine phosphorylation of paxillin and cytoskeletal reorganization were tightly coupled. Angiotensin II receptor occupancy has been shown previously to lead to protein kinase C activation. However, compared to angiotensin II stimulation, a smaller, delayed increase in paxillin tyrosine phosphorylation was observed following direct protein kinase C activation by the phorbol ester phorbol 12-myristate-13-acetate. Paxillin tyrosine phosphorylation was selective for certain agonists since no increase in tyrosine phosphorylation of this protein was observed following exposure to the potent mitogen PDGF. Thus, actin-based cytoskeletal changes involving sites of cell adhesion to the extracellular matrix may play an important role in normal and pathophysiologic smooth muscle cell growth regulation in response to certain angiotensin II-type vasoactive agonists.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  E. M. de Cavanagh, M. Ferder, F. Inserra, and L. Ferder Angiotensin II, mitochondria, cytoskeletal, and extracellular matrix connections: an integrating viewpoint Am J Physiol Heart Circ Physiol, March 1, 2009; 296(3): H550 - H558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hidaka, Y. Suzuki, M. Yamashita, T. Shibata, Y. Tanaka, S. Horikoshi, and Y. Tomino Amelioration of Crescentic Glomerulonephritis by RhoA Kinase Inhibitor, Fasudil, through Podocyte Protection and Prevention of Leukocyte Migration Am. J. Pathol., March 1, 2008; 172(3): 603 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Rembold, A. D. Tejani, M. L. Ripley, and S. Han Paxillin phosphorylation, actin polymerization, noise temperature, and the sustained phase of swine carotid artery contraction Am J Physiol Cell Physiol, September 1, 2007; 293(3): C993 - C1002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Zaidel-Bar, R. Milo, Z. Kam, and B. Geiger A paxillin tyrosine phosphorylation switch regulates the assembly and form of cell-matrix adhesions J. Cell Sci., January 1, 2007; 120(1): 137 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. McGarrigle, D. Shan, S. Yang, and X.-Y. Huang Role of Tyrosine Kinase Csk in G Protein-coupled Receptor- and Receptor Tyrosine Kinase-induced Fibroblast Cell Migration J. Biol. Chem., April 14, 2006; 281(15): 10583 - 10588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Tumbarello, M. C. Brown, S. E. Hetey, and C. E. Turner Regulation of paxillin family members during epithelial-mesenchymal transformation: a putative role for paxillin {delta} J. Cell Sci., October 15, 2005; 118(20): 4849 - 4863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Brown and C. E. Turner Paxillin: Adapting to Change Physiol Rev, October 1, 2004; 84(4): 1315 - 1339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Natarajan, G. Yin, and B. C. Berk Scaffolds Direct Src-Specific Signaling in Response to Angiotensin II: New Roles for Cas and GIT1 Mol. Pharmacol., April 1, 2004; 65(4): 822 - 825. [Full Text]
|
|
|
|
|
|
M. Sajid, R. Zhao, A. Pathak, S. S. Smyth, and G. A. Stouffer {alpha}v{beta}3-Integrin antagonists inhibit thrombin-induced proliferation and focal adhesion formation in smooth muscle cells Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1330 - C1338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-X. Liu, C. F. Yu, C. Nickel, S. Thomas, and L. G. Cantley Hepatocyte Growth Factor Induces ERK-dependent Paxillin Phosphorylation and Regulates Paxillin-Focal Adhesion Kinase Association J. Biol. Chem., March 15, 2002; 277(12): 10452 - 10458. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. T. Gerthoffer and S. J. Gunst Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle J Appl Physiol, August 1, 2001; 91(2): 963 - 972. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sul, C. B. Baron, R. Broome, and R. F. Coburn Smooth muscle length-dependent PI(4,5)P2 synthesis and paxillin tyrosine phosphorylation Am J Physiol Cell Physiol, July 1, 2001; 281(1): C300 - C310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D Hughes AT 1-signalling in vascular smooth muscle Journal of Renin-Angiotensin-Aldosterone System, June 1, 2000; 1(2): 125 - 130. [PDF]
|
|
|
|
 |
|
 V. Petit, B. Boyer, D. Lentz, C. E. Turner, J. P. Thiery, and A. M. Valles Phosphorylation of Tyrosine Residues 31 and 118 on Paxillin Regulates Cell Migration through an Association with Crk in Nbt-II Cells J. Cell Biol., March 6, 2000; 148(5): 957 - 970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Ford, S. Li, and J. G. Pickering Angiotensin II Stimulates Collagen Synthesis in Human Vascular Smooth Muscle Cells : Involvement of the AT1 Receptor, Transforming Growth Factor-{beta}, and Tyrosine Phosphorylation Arterioscler Thromb Vasc Biol, August 1, 1999; 19(8): 1843 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hassid, S. Huang, and J. Yao Role of PTP-1B in aortic smooth muscle cell motility and tyrosine phosphorylation of focal adhesion proteins Am J Physiol Heart Circ Physiol, July 1, 1999; 277(1): H192 - H198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takahashi, T. Taniguchi, H. Konishi, U. Kikkawa, Y. Ishikawa, and M. Yokoyama Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 1999; 276(6): H1927 - H1934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-Y. Zhu, C. S. Jones, S. Amin, K. Matsukuma, M. Haque, V. Vuligonda, R. A. S. Chandraratna, and L. M. De Luca Retinoic Acid Increases Tyrosine Phosphorylation of Focal Adhesion Kinase and Paxillin in MCF-7 Human Breast Cancer Cells Cancer Res., January 1, 1999; 59(1): 85 - 90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fang, R. V. Sharma, and R. C. Bhalla Enhanced Recovery of Injury-Caused Downregulation of Paxillin Protein by eNOS Gene Expression in Rat Carotid Artery : Mechanism of NO Inhibition of Intimal Hyperplasia? Arterioscler Thromb Vasc Biol, January 1, 1999; 19(1): 147 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Park, J. G. Borer, M. R. Freeman, and C. A. Peters Stretch activates heparin-binding EGF-like growth factor expression in bladder smooth muscle cells Am J Physiol Cell Physiol, November 1, 1998; 275(5): C1247 - C1254. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Brown, J. A. Perrotta, and C. E. Turner Serine and Threonine Phosphorylation of the Paxillin LIM Domains Regulates Paxillin Focal Adhesion Localization and Cell Adhesion to Fibronectin Mol. Biol. Cell, July 1, 1998; 9(7): 1803 - 1816. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. E. Aplin, A. Howe, S. K. Alahari, and R. L. Juliano Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins Pharmacol. Rev., June 1, 1998; 50(2): 197 - 264. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Ryan, H. C. Weber, W. Hou, E. Sainz, S. A. Mantey, J. F. Battey, D. H. Coy, and R. T. Jensen Ability of Various Bombesin Receptor Agonists and Antagonists to Alter Intracellular Signaling of the Human Orphan Receptor BRS-3 J. Biol. Chem., May 29, 1998; 273(22): 13613 - 13624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kaur, J. Yao, X. Pan, C. Matthews, and A. Hassid NO decreases phosphorylation of focal adhesion proteins via reduction of Ca in rat aortic smooth muscle cells Am J Physiol Heart Circ Physiol, May 1, 1998; 274(5): H1613 - H1619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takahashi, Y. Kawahara, T. Taniguchi, and M. Yokoyama Tyrosine phosphorylation and association of p130Cas and c-Crk II by ANG II in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, April 1, 1998; 274(4): H1059 - H1065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Brinson, T. Harding, P. A. Diliberto, Y. He, X. Li, D. Hunter, B. Herman, H. S. Earp, and L. M. Graves Regulation of a Calcium-dependent Tyrosine Kinase in Vascular Smooth Muscle Cells by Angiotensin II and Platelet-derived Growth Factor. DEPENDENCE ON CALCIUM AND THE ACTIN CYTOSKELETON J. Biol. Chem., January 16, 1998; 273(3): 1711 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Lutz, A. Piiper, H. Y. Gaisano, D. Stryjek-Kaminska, S. Zeuzem, and G. Adler Protein tyrosine phosphorylation in pancreatic acini: differential effects of VIP and CCK Am J Physiol Gastrointest Liver Physiol, December 1, 1997; 273(6): G1226 - G1232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Giasson, M. J. Servant, and S. Meloche Cyclic AMP-mediated Inhibition of Angiotensin II-induced Protein Synthesis Is Associated with Suppression of Tyrosine Phosphorylation Signaling in Vascular Smooth Muscle Cells J. Biol. Chem., October 24, 1997; 272(43): 26879 - 26886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Touyz and E. L. Schiffrin Angiotensin II Regulates Vascular Smooth Muscle Cell pH, Contraction, and Growth Via Tyrosine Kinase–Dependent Signaling Pathways Hypertension, August 1, 1997; 30(2): 222 - 229. [Abstract] [Full Text]
|
|
|
|
|
|
H. Abedi and I. Zachary Vascular Endothelial Growth Factor Stimulates Tyrosine Phosphorylation and Recruitment to New Focal Adhesions of Focal Adhesion Kinase and Paxillin in Endothelial Cells J. Biol. Chem., June 13, 1997; 272(24): 15442 - 15451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and H. S. Earp Paxillin Is Tyrosine-phosphorylated by and Preferentially Associates with the Calcium-dependent Tyrosine Kinase in Rat Liver Epithelial Cells J. Biol. Chem., May 30, 1997; 272(22): 14341 - 14348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Berk and M. A. Corson Angiotensin II Signal Transduction in Vascular Smooth Muscle : Role of Tyrosine Kinases Circ. Res., May 19, 1997; 80(5): 607 - 616. [Abstract] [Full Text]
|
|
|
|
|
|
K. K. Griendling, M. Ushio-Fukai, B. Lassegue, and R. W. Alexander Angiotensin II Signaling in Vascular Smooth Muscle: New Concepts Hypertension, January 1, 1997; 29(1): 366 - 370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Servant, E. Giasson, and S. Meloche Inhibition of Growth Factor-induced Protein Synthesis by a Selective MEK Inhibitor in Aortic Smooth Muscle Cells J. Biol. Chem., July 5, 1996; 271(27): 16047 - 16052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. O. De Nichilo and K. M. Yamada Integrin alpha(v)beta(5)-dependent Serine Phosphorylation of Paxillin in Cultured Human Macrophages Adherent to Vitronectin J. Biol. Chem., May 3, 1996; 271(18): 11016 - 11022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Shaw, C. E. Turner, and A. M. Mercurio The alpha6Abeta1 and alpha6Bbeta1 Integrin Variants Signal Differences in the Tyrosine Phosphorylation of Paxillin and Other Proteins J. Biol. Chem., October 6, 1995; 270(40): 23648 - 23652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Bellis, J. T. Miller, and C. E. Turner Characterization of Tyrosine Phosphorylation of Paxillin in Vitro by Focal Adhesion Kinase J. Biol. Chem., July 21, 1995; 270(29): 17437 - 17441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Guillemot, A. Levy, Z. J. Zhao, G. Bereziat, and B. Rothhut The Protein-tyrosine Phosphatase SHP-2 Is Required during Angiotensin II-mediated Activation of Cyclin D1 Promoter in CHO-AT1A Cells J. Biol. Chem., August 18, 2000; 275(34): 26349 - 26358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, R. Baron, and W. C. Horne Integrin Engagement, the Actin Cytoskeleton, and c-Src Are Required for the Calcitonin-induced Tyrosine Phosphorylation of Paxillin and HEF1, but Not for Calcitonin-induced Erk1/2 Phosphorylation J. Biol. Chem., November 17, 2000; 275(47): 37219 - 37223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Bellas, E. O. Harrington, K. L. Sheahan, J. Newton, C. Marcus, and S. Rounds FAK blunts adenosine-homocysteine-induced endothelial cell apoptosis: requirement for PI 3-kinase Am J Physiol Lung Cell Mol Physiol, May 1, 2002; 282(5): L1135 - L1142. [Abstract] [Full Text] [PDF]
|
|
