spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trevor, K. T.
Right arrow Articles by Leonova, E. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trevor, K. T.
Right arrow Articles by Leonova, E. V.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 108, Issue 1 343-356, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Association of vimentin intermediate filaments with the centrosome

KT Trevor, JG McGuire and EV Leonova
Center for Molecular Biology, Wayne State University, Detroit, Michigan, USA.

SW-13 cells that lack cytoplasmic intermediate filaments (IFs) were stably transfected with a human vimentin cDNA expression vector. Isolated subclones displayed two prevalent patterns of vimentin distribution as observed by indirect immuno-localization: (1) cytoplasmic filaments characteristic of a vimentin IF network; and (2) a distinct, juxtanuclear focus with limited filamentous extensions. Comparative analysis of two subclones that uniquely segregated these patterns of vimentin organization indicated that vimentin accumulated as a perinuclear focus in cells that expressed a 4-fold lower level of the protein. The observed variation in cellular organization was not due to detectable differences in vimentin protein modification, as determined by two-dimensional gel analysis. Increasing the amount of vimentin in a low expressing clone by a secondary transfection with human or mouse vimentin cDNA resulted in well-dispersed, cytoplasmic filaments, suggesting that the distinct juxtanuclear organization of vimentin arose due to lower cellular vimentin levels. Employing anti-gamma-tubulin and anti-vimentin antibodies, dual immunofluorescence together with confocal microscopy revealed that the juxtanuclear focus of vimentin was located in the centrosomal region. Electron microscopy showed a spheroidal, filamentous structure with at least some filaments closely associated with the pericentriolar material (PCM). Because vimentin IF organization is at least partially dependent on microtubules, the effects of nocodazole and taxol on perinuclear vimentin foci were examined. Neither drug affected the juxtanuclear localization of foci, although taxol (10 microM, 5 hours) caused a release of pericentriolar gamma-tubulin from the nuclear region in 50-60% of the cells. These studies indicate that lower, in vivo, levels of vimentin fail to form extended IFs but rather are organized as a perinuclear aggregate. Moreover, the PCM of the centrosome appears to possess attachment sites for vimentin IFs.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
A. S. Oriolo, F. A. Wald, G. Canessa, and P. J.I. Salas
GCP6 Binds to Intermediate Filaments: A Novel Function of Keratins in the Organization of Microtubules in Epithelial Cells
Mol. Biol. Cell, March 1, 2007; 18(3): 781 - 794.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
X. Yang, J. Wang, C. Liu, W. E. Grizzle, S. Yu, S. Zhang, S. Barnes, W. J. Koopman, J. D. Mountz, R. P. Kimberly, et al.
Cleavage of p53-Vimentin Complex Enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Mediated Apoptosis of Rheumatoid Arthritis Synovial Fibroblasts
Am. J. Pathol., September 1, 2005; 167(3): 705 - 719.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Sandal, L. Aumo, L. Hedin, B. T. Gjertsen, and S. O. Doskeland
Irod/Ian5: An Inhibitor of {gamma}-Radiation- and Okadaic Acid-induced Apoptosis
Mol. Biol. Cell, August 1, 2003; 14(8): 3292 - 3304.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C.-F. Hung, W.-F. Cheng, L. He, M. Ling, J. Juang, C.-T. Lin, and T-C. Wu
Enhancing Major Histocompatibility Complex Class I Antigen Presentation by Targeting Antigen to Centrosomes
Cancer Res., May 15, 2003; 63(10): 2393 - 2398.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Ameen, Y Figueroa, and P. Salas
Anomalous apical plasma membrane phenotype in CK8-deficient mice indicates a novel role for intermediate filaments in the polarization of simple epithelia
J. Cell Sci., January 2, 2001; 114(3): 563 - 575.
[Abstract] [PDF]

Home page
 JCBHome page
P. J.I. Salas
Insoluble {gamma}-Tubulin–containing Structures Are Anchored to the Apical Network of Intermediate Filaments in Polarized CACO-2 Epithelial Cells
J. Cell Biol., August 9, 1999; 146(3): 645 - 658.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1995