spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohara, R.
Right arrow Articles by Toyoshima, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohara, R.
Right arrow Articles by Toyoshima, K.

Journal of Cell Science, Vol 108, Issue 1 97-103, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Identification of the cells expressing cot proto-oncogene mRNA

R Ohara, S Hirota, H Onoue, S Nomura, Y Kitamura and K Toyoshima
Department of Oncogene Research, Osaka University, Suita, Japan.

The cell types expressing cot proto-oncogene mRNA were identified by in situ hybridization (ISH) histochemistry. Among a variety of adult mouse tissues examined, four types of glandular cells expressing cot gene were identified: (1) granular duct cells in the submandibular and sublingual glands; (2) serous cells in the parotid gland; (3) peptic (chief) cells in gastric glands; and (4) goblet cells in colonic glands. Investigation of the developmentally regulated expression of cot mRNA using tissues of 14-day and 18-day embryos, newborn and weanling mice showed that cot gene is expressed only in morphologically differentiated and functionally activated cells of these four types. No other types of cells showing ISH signals were observed. Based on these results, cot gene expressions in cultured cells of colonic adenocarcinomas and gastric adenocarcinomas were examined. SW 480 and WiDr cells showed high expression of this gene and so should be useful for functional analysis of Cot kinase. The expression patterns of cot gene in tumor tissues of the parotid gland, and gastric and colonic glands were investigated. Two of the tissues overexpressed this gene markedly, suggesting that overproduction of Cot kinase may be one cause of their transformation.


This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
A. Munshi, J. F. Kurland, T. Nishikawa, P. J. Chiao, M. Andreeff, and R. E. Meyn
Inhibition of constitutively activated nuclear factor-{kappa}B radiosensitizes human melanoma cells
Mol. Cancer Ther., August 1, 2004; 3(8): 985 - 992.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Kurland, D. W. Voehringer, and R. E. Meyn
The MEK/ERK Pathway Acts Upstream of NF{kappa}B1 (p50) Homodimer Activity and Bcl-2 Expression in a Murine B-Cell Lymphoma Cell Line: MEK INHIBITION RESTORES RADIATION-INDUCED APOPTOSIS
J. Biol. Chem., August 22, 2003; 278(34): 32465 - 32470.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. G. Eliopoulos, C. Davies, S. S. M. Blake, P. Murray, S. Najafipour, P. N. Tsichlis, and L. S. Young
The Oncogenic Protein Kinase Tpl-2/Cot Contributes to Epstein-Barr Virus-Encoded Latent Infection Membrane Protein 1-Induced NF-{kappa}B Signaling Downstream of TRAF2
J. Virol., March 27, 2002; 76(9): 4567 - 4579.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1995