spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laffafian, I.
Right arrow Articles by Hallett, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laffafian, I.
Right arrow Articles by Hallett, M. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 108, Issue 10 3199-3205, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Does cytosolic free Ca2+ signal neutrophil chemotaxis in response to formylated chemotactic peptide?

I Laffafian and MB Hallett
University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff, UK.

Cytosolic free Ca2+ concentration was measured and imaged in human neutrophils moving towards a source of formylated peptide in a micropipette held close to the cells. Under these conditions, neutrophils changed shape and displayed chemotaxis without significant or persistent global or localised elevations in cytosolic free Ca2+. A rear-to-front persistent Ca2+ gradient of less than 0.5 nM/micron was present in the migrating neutrophils, until they reached the zone of higher peptide concentration, when an abrupt rise in cytosolic free Ca2+ concentration was triggered and chemotaxis stopped. Small localised rises in cytosolic free Ca2+, which were occasionally observed during neutrophil manoeuvring, were attributed to the effect of local deformation of the neutrophil membrane, since deformation of the membrane with a blunt micropipette caused similar Ca2+ changes. These data suggest that neutrophil chemotaxis towards a source of formylated peptide occurs without significant changes in Ca2+ signalling.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
S. Dewitt, R. L. Darley, and M. B. Hallett
Translocation or just location? Pseudopodia affect fluorescent signals
J. Cell Biol., January 26, 2009; 184(2): 197 - 203.
[Abstract] [Full Text] [PDF]

Home page
 Phil Trans R Soc BHome page
B. K Rada, M. Geiszt, C. Hably, and E. Ligeti
Consequences of the electrogenic function of the phagocytic NADPH oxidase
Phil Trans R Soc B, December 29, 2005; 360(1464): 2293 - 2300.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Dewitt and M. B. Hallett
Cytosolic free Ca2+ changes and calpain activation are required for {beta} integrin-accelerated phagocytosis by human neutrophils
J. Cell Biol., October 14, 2002; 159(1): 181 - 189.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. A. Preobrazhensky, S. Dragan, T. Kawano, M. A. Gavrilin, I. V. Gulina, L. Chakravarty, and P. E. Kolattukudy
Monocyte Chemotactic Protein-1 Receptor CCR2B Is a Glycoprotein That Has Tyrosine Sulfation in a Conserved Extracellular N-Terminal Region
J. Immunol., November 1, 2000; 165(9): 5295 - 5303.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Pettit and M. Hallett
Release of 'caged' cytosolic Ca2+ triggers rapid spreading of human neutrophils adherent via integrin engagement
J. Cell Sci., January 8, 1998; 111(15): 2209 - 2215.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
A. Alteraifi and D. Zhelev
Transient increase of free cytosolic calcium during neutrophil motility responses
J. Cell Sci., January 8, 1997; 110(16): 1967 - 1977.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
S Yumura, K Furuya, and I Takeuchi
Intracellular free calcium responses during chemotaxis of Dictyostelium cells
J. Cell Sci., January 11, 1996; 109(11): 2673 - 2678.
[Abstract] [PDF]


© The Company of Biologists Ltd 1995