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Journal of Cell Science, Vol 108, Issue 10 3331-3337, Copyright © 1995 by Company of Biologists


JOURNAL ARTICLES

Identification of major proteins associated with Dictyostelium discoideum endocytic vesicles

C Adessi, A Chapel, M Vincon, T Rabilloud, G Klein, M Satre and J Garin
Laboratoire de Chimie des Proteines, CEA, Centre d'Etudes Nucleaires, Grenoble, France.

Magnetic isolation of endocytic vesicles from Dictyostelium discoideum was accomplished after feeding the amoebae with iron oxide particles. Proteins associated with the endocytic vesicles were resolved by SDS-PAGE and digested 'in-gel' with endoproteinase Lys-C or Asp-N to generate peptides for amino acid sequencing. This strategy allowed the identification of the major protein constituents of the vesicles: namely, the A, B, D, E and 110 kDa subunits of a vacuolar type H(+)-ATPase, actin, a Rab 7-like GTPase, a p34 protein corresponding to a new cysteine proteinase and the 25 kDa product of a recently sequenced D. discoideum open reading frame.


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