|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 11 3389-3396, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
CA Sparks, EG Fey, CA Vidair and SJ Doxsey
Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01605, USA.
NuMA, the nuclear mitotic apparatus protein, is a component of the nuclear matrix at interphase that redistributes to the spindle poles at mitosis. While the function of NuMA is not known, it has been implicated in spindle organization during mitosis and nuclear reformation. Phosphorylation is thought to play a regulatory role in NuMA function. In this study, NuMA phosphorylation was examined through the cell cycle using highly synchronized cells. In intact cells labeled with 32P-orthophosphate, NuMA appeared as a 250 kDa phosphoprotein in interphase that shifted to a higher apparent molecular mass in mitosis. The shift was due to phosphorylation as shown by reduction of the shifted band to interphase mobility by phosphatase treatment. This phosphorylation event occurred roughly at the G2/M transition at the time of NuMA's release from the nucleus and its redistribution to the mitotic spindle. However, mitotic phosphorylation did not require spindle formation since the phosphorylated species was detected in nocodazole-treated cells lacking microtubule spindles. Dephosphorylation of NuMA occurred in two distinct steps, after lamin B assembled into the nuclear lamina, in early G1 and at the end of G1. Based on the timing of the phosphorylation and dephosphorylation observed in this study, we propose that they may play a role in nuclear events such as nuclear organization, transcription, or initiation of DNA replication at G1/S.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. L. Nguyen and K. Munger Human Papillomavirus E7 Protein Deregulates Mitosis via an Association with Nuclear Mitotic Apparatus Protein 1 J. Virol., February 15, 2009; 83(4): 1700 - 1707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Si, S. C. Verma, M. A. Lampson, Q. Cai, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus-Encoded LANA Can Interact with the Nuclear Mitotic Apparatus Protein To Regulate Genome Maintenance and Segregation J. Virol., July 1, 2008; 82(13): 6734 - 6746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Kisurina-Evgenieva, G. Mack, Q. Du, I. Macara, A. Khodjakov, and D. A. Compton Multiple mechanisms regulate NuMA dynamics at spindle poles J. Cell Sci., December 15, 2004; 117(26): 6391 - 6400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Haren and A. Merdes Direct binding of NuMA to tubulin is mediated by a novel sequence motif in the tail domain that bundles and stabilizes microtubules J. Cell Sci., January 5, 2002; 115(9): 1815 - 1824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Young, J. B. Dictenberg, A. Purohit, R. Tuft, and S. J. Doxsey Cytoplasmic Dynein-mediated Assembly of Pericentrin and gamma Tubulin onto Centrosomes Mol. Biol. Cell, June 1, 2000; 11(6): 2047 - 2056. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Merdes, R. Heald, K. Samejima, W. C. Earnshaw, and D. W. Cleveland Formation of Spindle Poles by Dynein/Dynactin-dependent Transport of NuMA J. Cell Biol., May 15, 2000; 149(4): 851 - 862. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Melnick and J. D. Licht Deconstructing a Disease: RAR{alpha}, Its Fusion Partners, and Their Roles in the Pathogenesis of Acute Promyelocytic Leukemia Blood, May 15, 1999; 93(10): 3167 - 3215. [Full Text] [PDF]
|
|
|
|
|
|
M. Zecevic, A. D. Catling, S. T. Eblen, L. Renzi, J. C. Hittle, T. J. Yen, G. J. Gorbsky, and M. J. Weber Active MAP Kinase in Mitosis: Localization at Kinetochores and Association with the Motor Protein CENP-E J. Cell Biol., September 21, 1998; 142(6): 1547 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Dictenberg, W. Zimmerman, C. A. Sparks, A. Young, C. Vidair, Y. Zheng, W. Carrington, F. S. Fay, and S. J. Doxsey Pericentrin and gamma -Tubulin Form a Protein Complex and Are Organized into a Novel Lattice at the Centrosome J. Cell Biol., April 6, 1998; 141(1): 163 - 174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Saredi, L Howard, and D. Compton Phosphorylation regulates the assembly of NuMA in a mammalian mitotic extract J. Cell Sci., January 6, 1997; 110(11): 1287 - 1297. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Harborth, K. Weber, and M. Osborn GAS41, a Highly Conserved Protein in Eukaryotic Nuclei, Binds to NuMA J. Biol. Chem., October 6, 2000; 275(41): 31979 - 31985. [Abstract] [Full Text] [PDF]
|
|
