|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 11 3523-3530, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
EJ Williams, B Mittal, FS Walsh and P Doherty
Department of Experimental Pathology, UMDS, Guy's Hospital, London, UK.
We have cultured cerebellar neurons on monolayers of cortical astrocytes in control medium or medium containing recombinant basic fibroblast growth factor (FGF). FGF was found to inhibit neurite outgrowth, with a significant effect seen at 0.5 ng/ml and a maximal effect at 10 ng/ml. FGF increased the production of arachidonic acid (AA) in cerebellar neurons, and when added directly to cultures or generated endogenously via activation of phospholipase A2 using melittin, this second messenger could mimic the inhibitory effect of FGF. FGF and AA could also specifically inhibit neurite outgrowth stimulated by three cell adhesion molecules (NCAM, N-cadherin and L1) expressed in transfected fibroblasts, or in the case of L1 bound to a tissue culture substratum. These data demonstrate that, in certain cellular contexts, FGF can act as an inhibitory cue for axonal growth and that arachidonic acid is the second messenger responsible for this activity. We discuss the possibility that arachidonic acid inhibits neurite outgrowth by desensitising the second messenger pathway underlying neuronal responsiveness to cell adhesion molecules.
This article has been cited by other articles:
|
|
 |
|
  E.-J. Williams, F. S. Walsh, and P. Doherty The FGF receptor uses the endocannabinoid signaling system to couple to an axonal growth response J. Cell Biol., February 18, 2003; 160(4): 481 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Niethammer, M. Delling, V. Sytnyk, A. Dityatev, K. Fukami, and M. Schachner Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis J. Cell Biol., April 29, 2002; 157(3): 521 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Williams, E.-J. Williams, and P. Doherty Dimeric Versions of Two Short N-cadherin Binding Motifs (HAVDI and INPISG) Function as N-cadherin Agonists J. Biol. Chem., February 1, 2002; 277(6): 4361 - 4367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. F. Bearer, A. R. Swick, M. A. O'Riordan, and G. Cheng Ethanol Inhibits L1-mediated Neurite Outgrowth in Postnatal Rat Cerebellar Granule Cells J. Biol. Chem., May 7, 1999; 274(19): 13264 - 13270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Meiri, J. L. Saffell, F. S. Walsh, and P. Doherty Neurite Outgrowth Stimulated by Neural Cell Adhesion Molecules Requires Growth-Associated Protein-43 (GAP-43) Function and Is Associated with GAP-43 Phosphorylation in Growth Cones J. Neurosci., December 15, 1998; 18(24): 10429 - 10437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. B. Little, K. L. Crossin, L. A. Krushel, G. M. Edelman, and B. A. Cunningham A short segment within the cytoplasmic domain of the neural cell adhesion molecule (N-CAM) is essential for N-CAM-induced NF-kappa B activity in astrocytes PNAS, February 27, 2001; 98(5): 2238 - 2243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Niethammer, M. Delling, V. Sytnyk, A. Dityatev, K. Fukami, and M. Schachner Cosignaling of NCAM via lipid rafts and the FGF receptor is required for neuritogenesis J. Cell Biol., April 29, 2002; 157(3): 521 - 532. [Abstract] [Full Text] [PDF]
|
|
